...experiments.       References and resources References: Bonner WA, Hulett HR, Sweet RG, Herzenberg LA (1972) Fluorescence...

...recently spent two months in the lab of Antonello Bonci, Director of the National Institutes of Drug Abuse...

...for nearly any piece of DNA to be placed into a backbone vector of choice with minimal limitations. Background...MCS)), but do not cut elsewhere on the plasmid. Bonus: It is helpful to choose restriction enzymes that...have of uncut or self-ligating recipient plasmid backbone. Transformation Proceed with the transformation...

... pMAZ-SK contains an aTet-inducible gRNA and a backbone-targeting gRNA cassette for plasmid curing through...

...iPSC-derived osteoblasts and osteoclasts together promote bone regeneration in 3D biomaterials. Sci Rep, 2016. ...

...pyruvate, Corning 10-014-CV (optional) 7.5% sodium bicarbonate, Corning 25-035-CI (optional) 1 M HEPES, HyClone... 7.5 mL 100X glutaGRO, 7.5 mL of 7.5% Sodium Bicarbonate, 7.5 mL 1 M HEPES to 750 mL DMEM + 1 g/L glucose...other components, such as sorbitol and sodium bicarbonate, sorbitol has been found to increase viral titers...

...Cas-OFFinder will suffice. Cas-OFFinder provides a bare-bones data dump of ALL strings in a genome using any IUPAC...

...Fluorescent Protein Plasmids by Category Empty Backbones Understand the localization and/or function of...fluorescent proteins, as well as over 100 empty backbones. Fluorescent Protein Kits cpFRET Kit: FRET based...

...Imaging More Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Optogenetics Background...near-infrared , and photoactivatable fluorescent empty backbone plasmids are ideal for single or multicolor in...

...Collection Bacterial Expression Systems Empty Backbones Genetic Code Expansion Immunology Mammalian RNAi... are a set of plasmids all built with the same backbone and only differing in a small region. Click here...

...reaction which ligates a promoter into an empty backbone can be repeated to ligate your gene of interest...omitted from the coding sequence of the part and backbone. For some standards, there are additional sites...

...worm research. This includes CRISPR, RNAi, empty backbones, fluorescent proteins, and Cre expression plasmids... include plasmids expressing Cas9, empty gRNA backbones, and constructs for endogenous tagging. Some examples...

... pMAZ-SK contains an aTet-inducible gRNA and a backbone-targeting gRNA cassette for plasmid curing through...

...primers. These primers typically anneal to the backbone and can help you verify the ends of the insert...primers that are often found within the plasmid backbone (See Addgene's Sequencing Primers for reference...

...Cloning You may also like... Cloning Methods Empty Backbone Guide Molecular Biology Protocols Overview Ligation...design for LIC is often as simple as using the backbone manufacturer's suggested leader sequence fused...

...Nematode: Cas9 and gRNA use Cas9 (pIK86) ; gRNA empty backbone (pDR274) PDF 87.2 KB Liu Fly: gRNA cloning pAc-sgRNA-Cas9...sequencing Cas9: pMLM3613 dsDNA donor plasmid backbones pSL1180-HR-PUbECFP & pSL1180polyUBdsRED PDF 583.3...

...off-target editing is a major concern, Cas9-gRNA ribonucleoprotein (RNP) complexes are advantageous due to the... to generate transgenic embryos Cas9-gRNA ribonucleoprotein (RNP) complexes Purified Cas9 protein and ...

...packaging plasmid, expressing Rep2 and B1 capsid (backbone may yield higher titers than pAR-B1) Sena-Esteves..., and more. ID Plasmid Gene/Insert Vector Type Backbone Mutations Tags PI Do you have suggestions for ...

... 37 °C for 5 min. Note: Ribonuclease A (RNase A) is a pancreatic ribonuclease that digests single-stranded...

...Library 169942 Knockout Human Ebert 3rd 4 2,852 Bonifacino Lab Human ubiquitination-related proteins CRISPR...CRISPR KO library 174592 Knockout Human Bonifacino 3rd 6-25 11,108 Borodina Yeast Transporter Libraries...