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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 601 results for: arl

Showing: 241 - 260 of 601 results

  1. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...inspired you to perform this analysis. Having a clearly stated, specific goal for each analysis will help...student at University of Pennsylvania. She is particularly interested in science communication and epigenetics...

  4. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...Cas12a cleavage results in 5 bp overhangs and is particularly convenient for targeting experiments requiring...it targets RNA instead of DNA. This makes it particularly useful in generating transient edits of signaling...

  6. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ... also be used to ensure specificity; this is particularly helpful when wanting to target specific neuron...Dystrophy. InTech. doi: 10.5772/53023 Veldwijk, Marlon R., et al. "Development and optimization of a real-time...

  7. Addgene: The First Twenty Years

    Type
    Blog Post
    ...School. We want to give a special thanks to the early-adopter scientists, who trusted Addgene before we...— 10,000 plasmids deposited and available — and nearly seven before we had 1,000 depositing scientists...

  8. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...hairpin that can be designed to bind and detect nearly any RNA sequence. Downstream of the hairpin is ...26615910. PubMed Central PMCID: PMC4674677. Ronda, Carlotta, et al. "Metagenomic engineering of the mammalian...

  9. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...better serve the community. We are delighted at the early outcomes and the ecstatic reception by scientists...worry about getting support from depositors. We regularly hear how much they see the value in depositing...

  13. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ... direct ELISA, aside from the detection step. Similarly, the protein antigen is coated onto the wells ...calculate the protein concentration. Image from Jagarlamudi et al., 2015.   The maximum OD that most ...

  15. A Look at Addgene's QC Process

    Type
    Blog Post
    ...differences are considered a QC issue is reviewed yearly at a best practices meeting, where the QC team ...pipeline. There are regions of plasmids that are particularly difficult to sequence and assemble using the...

  16. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...Zibitt et al. 2021). Mutating splice sites is particularly effective as it can result in the retention ...used to target lncRNAs with high specificity, particularly in applications requiring smaller delivery systems...

  17. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...Why study neural connectivity? One of the early lessons many of us learned in biology is that the body...Deshpande et al., 2013). The study found that at early time points, most of the projections to these new...
Showing: 241 - 260 of 601 results