Addgene

We narrowed to 293 results for: IRES

Showing: 241 - 260 of 293 results

Results per page:

Overcoming the AAV Size Limitation for CRISPR Delivery

Type

Blog Post

...drawbacks limit the utility of these systems: St1Cas9 requires a very specific PAM sequence that limits the number...
Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

Type

Blog Post

...different reporter proteins. Protein translation requires the start codon of AUG immediately downstream ...
Analyzing CRISPR Editing Results with ICE from Synthego

Type

Blog Post

...generating reliable results from CRISPR edit data requires the help of robust software tools. As a consequence...
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

Type

Blog Post

...passages. Furthermore, working with Sendai virus requires stringent biosafety measures, thereby increasing...
How to Write a Scientific Review Article

Type

Blog Post

...convert the citation style to whatever the journal requires. Using the (Last name, Year) format also has the...
Plasmids 101: Gateway Cloning

Type

Blog Post

...and destination vector creates two products: the desires expression clone and a byproduct containing the...
Antibiotic Resistance: An Old Solution but a New Problem

Type

Blog Post

...Kirby-Bauer susceptibility test (Figure 2), and requires at least 18-24 hours to obtain results. While ...
Rett Syndrome: A History of Research and Therapeutic Outlooks

Type

Blog Post

...Samaco RC, Zoghbi HY (2011) Adult Neural Function Requires MeCP2. Science 333:186–186 . https://doi.org/10.1126...
Plasmids 101: Broad Host Range Plasmids

Type

Blog Post

...pneumophila, Methylophilus methylotrophus, Myxococcus virescens, M. xanthus, Proteus rettgeri, P. mirabilis, Providencia...
CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

Type

Blog Post

...initiation. In mammalian cells, robust repression requires targeting dCas9 along with transcriptional repressors...
The Fluorescent Vegetables in Aptamer Soup

Type

Blog Post

...instability at 37°C (Ouellet et al., 2016) and requires a tRNA scaffold to improve its stability in vivo...
SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

Type

Blog Post

...sequence preferences at the target site, while Cas12a requires a PAM (Protospacer Adjacent Motif) for target ...
Replacing Paper: Tips for Choosing an Electronic lab Notebook

Type

Blog Post

...switching from paper to an electronic lab notebook requires time and knowledge. In a recent article, we translated...
March for Science

Type

Blog Post

...science rallies scientists to political action, and inspires politicians and citizens to include science in...
Deep Mutational Scanning with One Pot Saturation Mutagenesis

Type

Blog Post

...phage-derived uracil labeled template, this method requires further DNA shuffling to combine pairs of mutations...
Antibodies 101: Introduction to Immunofluorescence

Type

Blog Post

...visualize their target. In contrast, indirect IF requires two antibodies: a primary and secondary. For indirect...
Antibodies 101: Single Chain Fragment Variables (scFvs)

Type

Blog Post

...for the scFv (Gaciarz and Ruddock., 2017), but requires an oxidizing environment to form. When expressed...
Tips for Getting a Faculty Position

Type

Blog Post

...really are selling yourself. A sales pitch that requires reading two pages or even a paragraph to get to...
Hot Biosensors 2022: Year-End Roundup

Type

Blog Post

...variations and heat transfer at the subcellular level requires a probe with fast kinetics and high signal/noise...
Antibodies 101: Beyond Surface Labeling

Type

Blog Post

... hence, intracellular labeling with antibodies requires chemically pre-treating the cells to allow for...

Showing: 241 - 260 of 293 results

Results per page:

Overcoming the AAV Size Limitation for CRISPR Delivery

Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

Analyzing CRISPR Editing Results with ICE from Synthego

pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

How to Write a Scientific Review Article

Plasmids 101: Gateway Cloning

Antibiotic Resistance: An Old Solution but a New Problem

Rett Syndrome: A History of Research and Therapeutic Outlooks

Plasmids 101: Broad Host Range Plasmids

CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

The Fluorescent Vegetables in Aptamer Soup

SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

Replacing Paper: Tips for Choosing an Electronic lab Notebook

March for Science

Deep Mutational Scanning with One Pot Saturation Mutagenesis

Antibodies 101: Introduction to Immunofluorescence

Antibodies 101: Single Chain Fragment Variables (scFvs)

Tips for Getting a Faculty Position

Hot Biosensors 2022: Year-End Roundup

Antibodies 101: Beyond Surface Labeling

Help Center

Deposit Plasmids

General Reagents

Viral Service

DNA Service

Plasmid Collections