...You've worked hard designing your plasmid – you carefully selected the optimal promoter for your gene of...recognize your little prokaryotic minions that carried out the labor-intensive process of replicating ...is appropriate for propogating your plasmid or carrying out your experiment. History of E. coli strains...metabolism pathway Inability to utilize arabinose as a carbon source leuB β-isopropyl malate dehydrogenase ...

...and we both won accolades that would launch our careers in science. Fast forward nearly 20 years and ... single author? I’ve seen so few in my 20+ year career that I can’t help but feel a bit dubious when I...Resources on Addgene.org Check out Career's at Addgene ... career...

...laboratory. The risk of generating RCL is low but extra care has to be taken especially if you are working with...of interest.   Advantages Disadvantages Can carry large transgenes (>8 kb) Potential for generation...feature for your experiment; it must be chosen carefully. Often researchers want a simple set-up where ...oncogenes for instance should be manipulated with care. See the NIH’s biosafety guidelines for more information...

...2007): 1740-1743. PubMed PMID: 17882145. 2. Zincarelli, Carmela, et al. "Analysis of AAV serotypes 1–9 mediated...make AAVs useful for a wide variety of experiments, care still needs to be taken when selecting which particular...22418061. PubMed Central PMCID: PMC3381869. 7. McCarty, D. M., Paul E. Monahan, and Richard J. Samulski...

... you can request any of over 3,000 eligible AAV cargo plasmids in the Addgene repository to be packaged...That way, you know you’re getting the serotype and cargo you requested at the quality you expect. Similar...serotype, place your order when ready, and we’ll take care of the rest.    We think Packaged on Request AAVs...

...prepared Tom for an academic career. Career advice for anyone pursuing a career in academia. Learning how...

...first to be infected in the United States while caring for a patient, despite wearing protective gear,...gear, and now comes news of a second Texas healthcare worker with symptoms. The World Health Organization... in EBOV’s past, present, and future, don’t miss Carl Zimmer’s coverage on the subject in the New York...

...chemogenetics, plasmids, fluorescent proteins, careers, and more. These resources can now be found in ...Voltage Indicators, and Photoswitchable Proteins by Cary Valley, Shreya Vedantam, Michelle Cronin, and Angela...fruit fly, yeast, zebrafish, and worm. 4. Early Career Researcher Toolbox: Free Tools for Making Scientific...

...Resources Science Careers: Improving your work-life balance Balancing the Scale: NSF's Career Life-Balance ...anyone leaving the lab receives send-off via a gift, card, and trip to the pub. Dr. Ellis adds “we probably...

...such as the NIH BEST award) and availability of career tools (such as the National Postdoc Association...Association Core Competencies and the Science Careers’ myIDP). Dr. Galit Lahav, Associate Professor of Systems ... to take advantage of all the possible science careers. She advocated for increasing the value and priority...

... to remove obstacles.” – Scott Adams, Dilbert cartoonist What makes a good manager? If that is all it ...is not the same as not being a bad manager (see cartoon above – what type of bad manager have you had?)...best managers have learned to get results with the carrot, and not the stick.     This is the first in a...

...ability to tell stories a similarly crucial skill for career advancement. Scientists do and should tell stories...can be learned, and will serve you well in your career. Story Telling at work Don’t believe me? Here’s...communication Find interviews scientists in SciComm careers Check out our series on transferable skills ...

...by Pediatrician Duangrurdee Wattanasirichaigoon carried different mutations in a gene called SOX10. To ...technology to create cell lines and/or mouse models carrying different SOX10 mutations. More specifically, ...kids in Thailand will receive much better health care in the future based on our studies.” Thank you to...

...confidence to launch new and diverse careers by taking part in career panels, and volunteering for scientific... “The Salmon Leap for PhDs: Swimming upstream a career from academia to industry”. To date, aside from...

...our excellent service and accuracy involves caring and careful Addgenies, but also a lot of good tracking... Bryan Roth’s lab from the University of North Carolina, Connie Cepko’s lab from Harvard Medical School...

...Cas9-expressing lentiviruses will no longer be carried over to the gRNA lentiviruses. This means that ...from Bryan Roth’s lab at the University of North Carolina, Chapel Hill. These can be used to investigate...pAAV-hSyn-DIO-hM4D(Gi)-mCherry contains a double floxed human muscarinic receptor 4 (Gαi-coupled) DREADD fused to mCherry...

... it, virtually every milestone in a scientist's career is mediated by some kind of communication to another...communication will be a positive asset throughout your career? 1. Make scicomm practice a priority The good news...Additional resources on the Addgene Blog Learn about careers in science communication The strength of story ...

...can then build on this research and effectively carry out their mission of creating new forums and opportunities...were generated by large agricultural companies and carry associated intellectual property concerns – patents...to construct our color changing flower we are carefully making our way through the IP landscape, using...

...that my research will have a direct impact on healthcare and the community. As a result, I have occasionally...target and light-up bacteria unwanted in food, healthcare settings, etc. What project in the Collins ...co-founder of a biotech startup, Mike explained, his career transitioned away from the bench and became all...

...makes them that way. Is there a senior person whose career path you are interested in following? Someone you...notify your network that you’re on the hunt for career advice. Your family and friends know you well and...groups will often have mentoring programs. The Healthcare Businesswomen’s Association hosts an excellent...