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  1. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...nucleotides of DNA can serve as the target for Cas9’s single guide RNA (sgRNA), provided it meets two conditions...system uses a “nicking” Cas9, in which one of Cas9’s two critical enzymatic residues has been converted...

  2. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...2412-2420. PubMed PMID: 25101481. Filonov, Grigory S., et al. "Broccoli: rapid selection of an RNA mimic...Central PMCID: PMC4923196. Panchapakesan, Shanker Shyam S., et al. "Ribonucleoprotein purification and characterization...

  3. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ... transposase. The donor plasmid contains the gene(s) of interest flanked by terminal repeat sequences,...They contain only a eukaryotic promoter and the cDNA(s) to be expressed and they don’t integrate or replicate...from this reference. 1. Malik, Nasir, and Mahendra S. Rao. "A Review of the Methods for Human iPSC Derivation...

  5. Antibody Production

    Type
    Collection
    ...embryonic kidney cells are transfected with the plasmid(s) encoding the heavy and light chains. Antibodies are...

  6. Chemogenetics AAV Preps

    Type
    Collection
    ...and Activity Reporter/Tag Cre requirement Serotype(s) PI Synapsin promoter 44361 pAAV-hSyn-DIO-hM3D(Gq)...

  7. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...iRFP713, % Oligomeric state Photo-stabilityc, t1/2, s pKa Brightness in HeLa cells vs. iRFP713, % d Ref...239 (2020). Oliinyk, O.S., Shemetov, A.A., Pletnev, S., Shcherbakova, D.M. & Verkhusha, V.V. Smallest near-infrared...

  9. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...involves making specific knock-out edits in a gene(s) of interest and has an average off-target frequency...CRISPR experiment? CRISPR 101: Cas9 The Other Cas(s) CRISPR 101: Validating Your Genome Edit         ...

  10. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    .... Liu XS, Wu H, Ji X, Stelzer Y, Wu X, Czauderna S, Shu J, Dadon D, Young RA, Jaenisch R (2016) Editing...plasmids from this publication at Addgene. Stolzenburg S, Beltran AS, Swift-Scanlan T, Rivenbark AG, Rashwan...

  11. Neurodegeneration Plasmid Collection

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    Collection
    ...TDP43 RRM-GFP sigma-S TARDBP CMV ALS Rajat Rohatgi 107799 pHBS931 TDP43 RRM-GFP F-S TARDBP CMV ALS Rajat...TDP43 RRM-GFP FYW-S TARDBP CMV ALS Rajat Rohatgi 118792 pHBS1385 TDP43 RRM-GFP VLIM-S TARDBP CMV ALS Rajat...pHBS1410 GFP-TDP43 F-S TARDBP CMV ALS Rajat Rohatgi 118800 pHBS1411 GFP-TDP43 FYW-S TARDBP CMV ALS Rajat...BFP-P2A-TDP43 F-S] TARDBP CMV ALS Rajat Rohatgi 118809 pHBS1404 [IBB-GFP-mCherry 3E]-[BFP-P2A-TDP43 VLIM-S] TARDBP...SUMO-TDP43 F-S-TEV-mCherry TARDBP T5 ALS Rajat Rohatgi 133322 pHBS1552 H14-SUMO-TDP43 FYW-S-TEV-mCherry...-TDP43 F-S] TARDBP CMV ALS Rajat Rohatgi 133329 pHBS1505 [IBB-GFP-mCherry3E]-[BFP-TDP43 FYW-S] TARDBP ...Rajat Rohatgi 107800 pHBS935 TDP43 RRM-GFP M-S TARDBP CMV ALS Rajat Rohatgi 107801 pHBS934 TDP43 RRM-GFP...

  12. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...orthogonal nuclease-inactive Cas9's (dCas9) from S. pyogenes, N. meningitidis and S. thermophilus which have been...favorite cells. Ma et al., Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112...620nm) portions of the spectrum. PhD student Benjamin S. Padman recently developed a mitochondrially-targeted...context. Chatterjee A et al. Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):11803-8. Plasmid tools for...

  13. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...lately (1, 2), depends on knowledge of the structure(s) a given phage will recognize on a host cell. To ..., Callum J., Mohammadali Khan Mirzaei, and Anders S. Nilsson. "Adapting drug approval pathways for bacteriophage-based...

  14. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...topics. Resources and references References Aydin S (2015) A short history, principles, and types of ELISA...10.1016/0003-2697(81)90281-5 Pillai-Kastoori L, Heaton S, Shiflett SD, Roberts AC, Solache A, Schutz-Geschwender...

  15. Antibodies 101: Validation

    Type
    Blog Post
    ...like Antibodypedia or through your antibody provider(s) if they accept and share user data on the antibody...10.1016/j.nbt.2018.08.002  Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, Rodriguez...

  16. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...as effective at editing mouse embryos as Cas9 from S. pyogenes and has the advantage of being smaller (...health.     References Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini...
Showing: 241 - 260 of 413 results