...NGAG and NGCG and SaCas9 with an NNGRRN PAM by mutagenizing the PAM-interacting domain. However, these alternative...mutants with PAM flexibility. In this system, the mutagenesis plasmid (MP6) permits quick, large-scale mutation...recognizing multiple PAMs should have a fitness advantage over proteins with single PAM recognition. ...cell population measured with NGS) are shown as percentages, with fold changes compared to SpCas9 in parentheses...Plasmids for Mammalian Systems Learn about CRISPR History ...

...on the Addgene blog Learn about the advantages and disadvantages of using monoclonal antibodies Read ...pathogen. Different isotypes also appear at different stages of an immune response. There are three main pathogen...antibody-based applications including flow cytometry, immunohistochemistry, immunocytochemistry, ELISAs, and Western...

...Efficient and Stable Genome integration Insertional mutagenesis, transgene reactivation, residual expression ...Efficient and Stable Genome integration Insertional mutagenesis, residual expression piggyBac Excisible Medium...reprogramming efficiencies reported in González et al. A percentage is not listed because many variables (cell type...generating methods due to the risk of insertional mutagenesis. Random integration also creates heterogeneous...Finally, retroviruses are prone to insertional mutagenesis and random integration creates variation between...because they are less likely to cause insertional mutagenesis, adenoviral vectors are considered a safer way...These methods eliminate the risk of insertional mutagenesis, the presence of a genetic scar, and incomplete...

...time and money gets put into them in those later stages. Target identification Not all diseases are fully...are selected.      CRISPR aids in multiple stages of the drug discovery pipeline.   CRISPR ...CRISPR Guide CRISPR Plasmids and Resources CRISPR History and Development for Genome Engineering    ...

...observed in the kidneys at a specific developmental stage you could infer that the gene which GFP was monitoring...throughout an organism’s genome. These reporters have historically been used extensively in fly, mouse, and yeast...Development-specific genes – if a gene functions at multiple stages in development, its depletion may result in lethality...

...reagents. But for a quick refresher, some of the advantages of sdAbs over conventional antibodies include... simple structure    sdAbs in research These advantages have contributed to sdAbs becoming valuable tools...Arbabi-Ghahroudi M (2017) Camelid Single-Domain Antibodies: Historical Perspective and Future Outlook. Front Immunol...

...Numerous applications have been developed to take advantage of the high fidelity of Taq and other thermostable...and even include profiling single nucleotide polymorphisms (SNPs) (5,12,13). For example, in the Ligase...for characterization of resistance-mediating polymorphisms in African samples of Plasmodium falciparum....

...graduate with my BSc in Anatomy, the University of Otago had established the Centre for Science communication...flourish. I’ve never forgot about all the anxious, whispered questions from friends and classmates back in ...Department of Science Communication at the University of Otago. Her research focuses on misinformation about sexual...

...tuned” with mutagenesis to extend at different pH values. Using a screen that takes advantage of the fact...enormous lattice of identical subunits. A brief history of R bodies R bodies have been studied for many...

...characterized orthologs from “Anaerobic digester metagenome” (AdmCas13d) and Ruminococcus flavefaciens (nicknamed...immunogenicity. But these studies also identified other advantages, including Cas13d-specific regulatory proteins...Plasmids Check out our CRISPR Guide Read our CRISPR History  ...

...of the biosensors used in research are very sophisticated, but at their core they can all be boiled down...Cellular phenomena such as exocytosis, autophagy, voltage, and tension all have specific sensors designed...good thing, though! Viral delivery has several advantages over other mechanisms. For one, many viral platforms...

...ccdB accomplish this? Let’s start with a brief history of the gene and how molecular biologists have harnessed...modern version of this older system, with added advantages that will be discussed in detail in a separate...post. Focusing on the ccdB aspect, Gateway takes advantage of the same principle that cells will not propagate...

...Best for: very large deletions.   Cas12a – The staggered cutter  Originally classified as Cpf1, Cas12’s...primary difference from Cas9 is that it generates staggered breaks, also known as sticky ends. Cas12a cleavage...to detect variants such as single nucleotide polymorphisms. Fast facts – PAM requirement: none. Best ...

...repurpose popular GFP reporter lines for more sophisticated experimental manipulations, saving the time ...optogenetics in specific cell populations even easier. Advantages and other possibilities It’s clear that Cre-DOG...GFP-labeled lines, and they each have their own advantages. T-DOGGs can be made drug-inducible using the...

... consultation meeting or call for free.  Take advantage of this to get the information you need to choose... and speaker is Gwen Acton of Vivo Group whose tagline is Bridging Science and Business. Maybe you just...has an outstanding blog and, while her PhD is in History, I think she would be a good match as a coach for...

...: Sign up for a Twitter account. Check out the hashtag #scicomm and start following science communication...local pub at a Pint of Science event. Or get on stage for a Science Slam – a sort of competitive TED talk...or upload your data to Zooniverse. The Natural History Museum has downloadable resources to enable you...

...science, dating back several hundred years, with historical ties to psychology and philosophy (the mind-body...generally mediates broad cell tropism and has a high percentage of neuronal targeting after stereotaxic injection...Alternatively, for larger brain areas, repeated staggered injections may be considered. Timing: High expression...

...cleavage in human cells. Mutagenesis, 20(2), 111–114. https://doi.org/10.1093/mutage/gei016I Resources on ...Ampicillin Ampicillin, often just called amp, is the historical king of the antibiotic resistance realm. Amp ...

... popular and useful viral vectors in the lab. Advantages of lentivirus include a large genetic capacity...applications of lentiviral vectors! Lentiviral vector history and components In the early 1990s, researchers ...research is still in the pre-clinical/early clinical stages. Researchers are also working to make lentiviral...

...bacteria, the tumor cells that escape the elimination stage acquire genetic alterations and alter cell-surface..., by way of favorably attaining the equilibrium stage. This can be called “stalemate equilibrium” where...the acute immune responses that eliminate early stage cancer cells, chronic inflammatory responses can... downregulate the expression of the major histocompatibility complex (MHC) class I proteins on their surfaces...