...devised methods to deliver CRISPR components as ribonucleoproteins (RNPs) that can be supplied separately from...

...the fluorescent protein collection Find empty backbones for fluorescent protein fusions Browse the fluorescent...

... Panchapakesan, Shanker Shyam S., et al. "Ribonucleoprotein purification and characterization using RNA...

...the Fluorescent Protein Collection Find empty backbones for fluorescent protein fusions Browse the Fluorescent...

...the desired mutation and excision of the vector backbone. This step requires “counter-selection,” meaning...

...How to Design Your gRNA Delivering CRISPR Ribonucleoproteins Resources on Addgene.org Browse All CRISPR...

...create the pCell-Free vectors, Gateway-compatible backbones that enable cell-free expression of proteins in...

...hiring manager or host for topic advice. An added bonus to knowing your audience is that it can help you...

...still think “but that won’t happen to me, I have a backbone”. The sad truth is, if it happened before, it ...

...typically containing thiols, to cleave disulfide bonds. Load the samples, one sample per lane, onto the...

... (single copy, bacterial artificial chromosome backbone) contains the register. Depending on the desired...

...activity and instead become trapped in a covalent bond with its substrate. Then, all it takes is a bit ...

... way of creating and strengthening those social bonds. So never mind how much better your work and productivity...

...construct, where your RNAi-target is fused to the DNA backbone that contains a target sequence against the fluorescent...

...prokaryotic survival, but it is not invincible. Bondy-Denomy et al. identified the first set of anti-CRISPR...prokaryotes. Science . 315(5819):1709-12. PMID: 17379808 Bondy-Denomy J, Garcia B, Strum S, Du M, Rollins MF, Hidalgo-Reyes...proteins. Nature . 526(7571):136-9. PMID: 26416740 Bondy-Denomy J, Pawluk A, Maxwell KL, Davidson AR. 2013...

... Visualization Technique/Tag PI Purpose Empty Backbones with Fluorescent protein tags Various Plasmids...interest to the periplasm which may aid disulfide bond formation or prevent toxicity. Compatible with Gateway...as tags for protein localization to aid disulfide bond formation F Xavier Gomis-Ruth Kit containing plasmids...

...what vector to use, you can check out our Empty Backbone Reference . Background Subcloning by restriction...and purified your insert and recipient plasmid backbone bands away from the gel via your favorite gel ...have of uncut or self-ligating recipient plasmid backbone. Transformation Transform your ligation reaction...

...Addgene.org Find Photoactivatable FPs in Empty Backbones in the Michael Davidson Collection Find Photoactivatable...

...Laderman, E., Armbrust, M., Hallmark, T., Keiser, D., Bondy-Denomy, J., & Jackson, R. N. (2021). Positioning...

...Protein Resources Find Empty Fluorescent Protein Backbones Find Plasmids for Optogenetics ...