We narrowed to 601 results for: arl
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TypeBlog Post...Alpha variant), B1.1.298, and B.1.429 (Epsilon), nearly as well as they could for the wild-type. However...TM, Clayton KL, Nitido AD, Murali MR, Alter G, Charles RC, Dighe A, Branda JA, Lennerz JK, Lingwood D,...
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Single-cell tracking of lineage and identity with CellTag
TypeBlog Post...time point, making it difficult to investigate how early expression patterns influence reprogramming outcomes...However, distinct transcriptional states were seen as early as day 6. All together these results suggest that... -
What the HEK?
TypeBlog Post...two areas of research where HEK cells may be particularly useful due to their intrinsic properties. ...by mycoplasma and bacteria, but HEKs are also particularly vulnerable to viral infection. Yes, your cells... -
New and Upcoming Viral Vectors - December 2019
TypeBlog Post...We have also packaged hChR2(H134R) plasmids from Karl Deisseroth’s lab, which includes pAAV-hSyn Con/Fon...WPRE-SV40 124650 AAV9 pAAV-CamKIIa-C1V1(t/t)-mScarlet-KV2.1 55645 AAV5 pAAV-hSyn Con/Fon hChR2(H134R... -
Donations from Addgene to Yield Answers for Rare Disease Researchers
TypeBlog Post...by mutations in the OCRL1 gene that leads to the early death of young patients as their kidneys fail. The...interested in XP out of a desire to understand the early events in the transformation of healthy cells into... -
Creating accessible biology activities in schools with BioBits
TypeBlog Post...thinking: I wish I had this kind of experience earlier! After all, students build circuits in physics... about operating the rest of the car as well. Similarly, cell free expression systems contain all the ... -
Plasmids 101: Gram Negative and Positive Bacteria
TypeBlog Post...decolorized as gram negative. A German scientist, Carl Weigert, later expanded on Gram’s discovery, adding...resources. Bruckner, M. Gram Staining. https://serc.carleton.edu/microbelife/research_methods/microscopy/gramstain.html... -
Using CRISPR/Cas9 to Edit Disease Out of the Genome
TypeBlog Post...They are targeting the genes responsible for both early onset and late onset AD. In this study, the group...autosomal-dominant mutations that have been connected to the early onset of Alzheimer’s disease, as well as those that... -
Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond
TypeBlog Post... neutralize the SARS-CoV-2 virus. But while the early results were promising, the researchers did not ... the preprint repository BioRxiv. This example clearly illustrates the power of collaboration, which despite... -
Celebrate National Book Month with Addgene
TypeBlog Post...insects, mammals, and plants, among others. I particularly enjoyed the emphasis that microbes are neither...last 8 years. Since then, we’ve read books across nearly every genre you can imagine: gothic, mystery, sci-fi... -
Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System
TypeBlog Post... 2017. These travel awards are intended to help early career scientists attend a conference of their choice...evidence suggests that it is incredibly important for nearly every aspect of life.” Unger began with the a bacterial... -
The MTA at Addgene
TypeBlog Post...MTAs by the Provider and Recipient (in Addgene parlance, the depositor and requestor) organizations. Over...depends on things being organized, tracked, and clearly communicated. And that’s where MTAs, and similar... -
Addgene Welcomes John Doench to the Advisory Board
TypeBlog Post...specificity in the miRNA regulatory process. This early research is still relevant to his work at the Broad...screens to learn more about cancer biology in Ed Harlow’s group at Harvard Medical School and then applied... -
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog Post...research (3). The sky is the limit. As mentioned earlier, even genes not found in Drosophila can be studied...immunology PhD at Harvard University. He has had nearly a decade of experience working with Drosophila ... -
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors
TypeBlog Post...Therapies: CRISPR, Stem Cells, and Beyond meeting earlier this month. Another issue that arises for base...cannot be expressed until the base editor corrects early stop codons within T7 RNAP. A phage encoding the... -
Plasmids 101: Inducible Promoters
TypeBlog Post...between cell types or organisms, promoters are similarly variable. Bacterial promoters only work in prokaryotic...related species from which they were derived. Similarly, the various eukaryotic cell types (mammalian,... -
Antibodies 101: Isotypes
TypeBlog Post... in Health and Disease. 5th edition. New York: Garland Science; 2001. Structural variation in immunoglobulin... in Health and Disease. 5th edition. New York: Garland Science; 2001. The distribution and functions of... -
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME
TypeBlog Post...miRNA reporter podcast segment Discovered in the early 1990’s, microRNAs (miRNAs) are recognized as important...analysis of immune cells has been a gap in the field. Arlene Sharpe’s group at the Harvard Medical School developed... -
Viral Vectors 101: Inverted Terminal Repeats
TypeBlog Post...because they demonstrate high promoter activity (Earley et al. 2020), but non-AAV2 ITRs can be used to ...microorganisms1202031038399714. PMID: 38399714. Earley, L. F., Conatser, L. M., Lue, V. M., Dobbins, A... -
Behind the scenes: Addgene’s new search engine and more
TypeBlog Post...results. The footer and improved menus we launched earlier in the year, as well as our redesigned homepage...categorization of our information in order to more granularly customize the search engine, and a scoping mechanism...