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  1. The MTA at Addgene

    Type
    Blog Post
    ...MTAs by the Provider and Recipient (in Addgene parlance, the depositor and requestor) organizations. Over...depends on things being organized, tracked, and clearly communicated. And that’s where MTAs, and similar...

  2. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...because they demonstrate high promoter activity (Earley et al. 2020), but non-AAV2 ITRs can be used to ...Addgene's AVV guide AAV Viral Preps References  Earley, L. F., Conatser, L. M., Lue, V. M., Dobbins, A...

  3. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...decolorized as gram negative. A German scientist, Carl Weigert, later expanded on Gram’s discovery, adding...resources.   Bruckner, M. Gram Staining. https://serc.carleton.edu/microbelife/research_methods/microscopy/gramstain.html...

  4. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...They are targeting the genes responsible for both early onset and late onset AD. In this study, the group...autosomal-dominant mutations that have been connected to the early onset of Alzheimer’s disease, as well as those that...

  6. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...results. The footer and improved menus we launched earlier in the year, as well as our redesigned homepage...categorization of our information in order to more granularly customize the search engine, and a scoping mechanism...

  8. Antibodies 101: Isotypes

    Type
    Blog Post
    ... in Health and Disease. 5th edition. New York: Garland Science; 2001. Structural variation in immunoglobulin... in Health and Disease. 5th edition. New York: Garland Science; 2001. The distribution and functions of...

  10. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...between cell types or organisms, promoters are similarly variable. Bacterial promoters only work in prokaryotic...related species from which they were derived. Similarly, the various eukaryotic cell types (mammalian,...

  11. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...inspired you to perform this analysis. Having a clearly stated, specific goal for each analysis will help...student at University of Pennsylvania. She is particularly interested in science communication and epigenetics...

  14. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...hairpin that can be designed to bind and detect nearly any RNA sequence. Downstream of the hairpin is ...26615910. PubMed Central PMCID: PMC4674677. Ronda, Carlotta, et al. "Metagenomic engineering of the mammalian...

  16. Celebrating 15 Years of Scientific Sharing

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    Blog Post
    ...better serve the community. We are delighted at the early outcomes and the ecstatic reception by scientists...worry about getting support from depositors. We regularly hear how much they see the value in depositing...

  18. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ... also be used to ensure specificity; this is particularly helpful when wanting to target specific neuron...Dystrophy. InTech. doi: 10.5772/53023 Veldwijk, Marlon R., et al. "Development and optimization of a real-time...

  19. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...Cas12a cleavage results in 5 bp overhangs and is particularly convenient for targeting experiments requiring...it targets RNA instead of DNA. This makes it particularly useful in generating transient edits of signaling...
Showing: 221 - 240 of 560 results