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Showing: 221 - 240 of 254 results
  1. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Titration Assay Lentivirus Fluorescence Titration Assay AAV Titration by qPCR Addgene Viral Service Lentivirus...
  2. Tetracycline Inducible Expression

    Type
    Collection
    ...pTREtight promoter None Either Elledge 35625 pAAV-Ptet-RFP-shR-rtTA AAV; shRNA cloning vector; for evaluation... interest. tTA Off Sabatini 92099 AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep Bidirectional promoter controls expression...System. Contains homology arms for integration into AAVS1 Genomic Safe Harbor Locus. Tet-On 3G On Doyon 58245...
  3. Science Guides

    Type
    Guide
    ...research, including: Lentivirus Adeno-associated Virus (AAV) Adenovirus γ-Retrovirus Read More...
  4. Deisseroth INTRSECT Collection

    Type
    Collection
    ...targeting strategy that allows adeno-associated virus (AAV)-borne payloads to be expressed in cells based on...55636 pAAV-EF1a-Cre None 55632 pAAV-Ef1a-mCherry-IRES-Cre None 55637 pAAV-EF1a-Flpo None 55634 pAAV-EF1a-mCherry-IRES-Flpo...pAAV-EF1a-mCherry-IRES-Flpo None 55638 pAAV-EF1a-vCre None 55635 pAAV-EF1a-sCre None 55633 pAAV-EF1a-mCherry-IRES-Dre ...Mutations 55641 pAAV-Ef1a-fDIO EYFP Flp 55640 pAAV-Ef1a-dDIO hChR2(H134R)-EYFP Dre 55639 pAAV-Ef1a-fDIO hChR2...Cre 137135 pAAV-Ef1a-oScarlet None 137136 pAAV-Ef1a-Con/Fon-oScarlet Cre AND Flp 137137 pAAV-Ef1a-Con/Foff... Cre 137125 pAAV-Ef1a-sRGECO None 137126 pAAV-Ef1a-Con/Fon-sRGECO Cre AND Flp 137127 pAAV-Ef1a-Con/Foff...hChR2(H134R)-EYFP Flp 126080 pAAV-Ef1a-sCreDIO hChR2(H134R)-eYFP Scre 126081 pAAV-Ef1a-vCreDIO hChR2(H134R...
  5. Deep Dive: qPCR

    Type
    Blog Post
    ...resources on the Addgene blog Digital Droplet PCR for AAV QuantificationPlasmid Cloning by PCR...
  6. Molecular Biology Reference

    Type
    Guide
    ...viral particles, such as lentiviral, retroviral, AAV, or adenoviral particles, that can infect your target...expanding viral service offers select ready-made AAV and lentiviral particles. Visit our viral service...
  7. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...113584 (EFS) 113585 (TBG) Knockout Mouse Chen N/A (AAV) 4 286 Mouse Validation (mVAL) CRISPR Library 159391...
  8. Plasmids for Stem Cell Research

    Type
    Collection
    ...Jun 6. Buganim Glial Cells Retinal Ganglion Cells AAV/CRISPR Mouse Glia-to-Neuron Conversion by CRISPR-...
  9. CRISPR Guide

    Type
    Collection
    ...efficiently packaged into adeno-associated viruses (AAVs) . Other natural Cas orthologs include Hsp1Cas9 ...Mammalian CRISPR libraries have also been created in AAV backbones for in vivo experiments and in a retroviral...capabilities but are small enough to be packaged in AAV particles. Cas13 fusions have also been used in in...
  10. CRISPR Guide

    Type
    Guide
    ...efficiently packaged into adeno-associated viruses (AAVs) . Other natural Cas orthologs include Hsp1Cas9 ...Mammalian CRISPR libraries have also been created in AAV backbones for in vivo experiments and in a retroviral...capabilities but are small enough to be packaged in AAV particles. Cas13 fusions have also been used in in...
  11. Our New Antibody Mascot is....

    Type
    Blog Post
    ...  Last month, we introduced you to Blugene and Aavery's newest friend, our Addgene antibody mascot, here...on Feb. 24. Stay tuned!    Original Blugene and Aavery would like your attention, please, as they introduce...AntibodyMakesThree on Twitter. As with Blugene and Aavery, we’re looking for a gender neutral name (and it...
  12. General Transfection

    Type
    Protocol
    ...of HEK293T cells optimized for viral production (AAVpro or Lenti-X), but any HEK293T cell line should work...
  13. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...-FP Plasmid Type Mammalian, non-viral Lentiviral AAV Retroviral Bacterial Yeast Other Promoter CMV T7 ...74170 pVEX-CC1 DCTN1 tac ALS Trina Schroer 75437 p-AAV-sh[SNCA] SNCA U6 Parkinson's Edward Burton 75637 ...Parkinson's Hilal Lashuel 36055 pAAV asyn WT SNCA CMV Parkinson's Hilal Lashuel 36056 pAAV asyn S87A SNCA CMV Parkinson's...Hilal Lashuel 36066 pAAV asyn S129A SNCA CMV Parkinson's Patrick Aebischer 36067 pAAV asyn S129G SNCA CMV...Hilal Lashuel 36068 pAAV asyn S129E SNCA CMV Parkinson's Hilal Lashuel 36069 pAAV asyn S129D SNCA CMV ...Aebischer 36070 pAAV asyn S87A/S129A SNCA CMV Parkinson's Patrick Aebischer 36071 pAAV asyn S87D/S129A...Lashuel 107543 pAAV-AICD-NLS-IRES-hrGFP APP CMV Alzheimer's Hélène Marie 107544 pAAV-AICD-NES-IRES-hrGFP...
Showing: 221 - 240 of 254 results