...study a matrix metalloproteinase: López-Pelegrín, Mar, et al. "A novel mechanism of latency in matrix metalloproteinases...Addgene. What you might not know is that Addgene distributes curated collections of plasmids as kits with ...This kit is designed to be used with Type IIS restriction enzymes and standardized primers to clone your... pExpression vectors) plasmids. The type IIS restriction sites used in these vectors are of low abundance...Biosafety of biotechnologically important microalgae: intrinsic suicide switch implementation in cyanobacterium...used to generate these fusions all use the same restriction enzymes for cloning. Furthermore, some plasmids...

...collaboration. Together, we distributed 184,824 materials to 39,529 scientists across 86 countries. Even more remarkably...remarkably, 1,160 labs from 522 organizations in 41 countries enriched our repository with 15,020 new plasmids...part of the story. This year, we made significant strides in making science more accessible and reliable....thoroughly characterized antibodies, and our contributions to key research papers are already inspiring...Errington, T. M., Turn, R. E., Bandrowski, A., Trimmer, J. S., Rego, M., Freedman, L. P., Ferrara, F.,...

...of months. Read on to discover what new post contributed the most to this record breaking month and to... Apps for Lab Life This very useful post was contributed by guest blogger Sean Stacey from Eastern Tennessee...Phage Genomes for Host Binding Proteins," was contributed by guest blogger Jessica Sacher from the University...Disease, Running for FOP, Running for AJ,” was contributed by Kurt Swanson, husband of Addgene Director ...currently on my way back from a very successful Outreach Trip in San Francisco and would like to give my thanks...who spoke with me and deposited plasmids on this trip. Here’s to hoping that October is even more awesome...

... In 2023, Addgene distributed 188,729 materials to 39,844 scientists in 88 countries around the world....1,106 labs in 504 organizations from 37 different countries, into our repository, while expanding global access... access to our repository through our distributor partners, with the launch of antibodies in China and...Protein Innovation (IPI), in which Addgene will distribute recombinant antibodies (rAbs) developed at IPI... repository, partially through work with our distributors in China, Korea, Japan, India, and Brazil. Through...

... and were only available as green probes. The Patriarchi Lab aimed to overcome these limitations by developing...vivo experiments, and nLightG was successfully trialed in two-photon in vivo experiments. nLight indicators...nLightR, both generating a measurable signal after electrical stimulation with a single pulse, while nLightR...researchers looking to study norepinephrine, the Patriarchi lab continues to work on further improvements...nLightG. Many thanks to Mike Waring and Tommaso Patriarchi for their assistance with this blog post. References...Benke, D., Weber, B., Bohacek, J., Reiner, A., … Patriarchi, T. (2023). Sensitive multicolor indicators for...

...includes 1049 community-validated reagents from 20 contributors, including 61 fluorescent probes, representing...for the performance of a reagent, e.g., antigen retrieval conditions, dye inactivation details, tissue preservation...   Figure 2: Screenshot showing reagent entries with associated metadata adapted from the Affinity...preservation, target tissue, detergent, antigen retrieval conditions, and more. Users can also recommend... showing the IBEX Knowledge-Base, with every contributor listed as an author.  The IBEX Knowledge-...and share their validation work. Everyone who contributes to the IBEX Knowledge-Base is named on the Zenodo...

... submissions via email: [email protected] Entries include accession number, species, and assigned...cell lines profiled in the lab. You can browse entries by cell line groups, such as their vaccine production...collections.  Depending on available information, entries may also include whether a cell line is known to...programatically, their API allows fine grained query and retrieval of all the information in the resource.  This ...cells. Image courtesy of Josef Reischig.  Contributing to Cellosaurus Generated a new cell? Or finding...sent the updated information to the cell line distributor. A success all around! If you’re excited about...

... Kolmer-Agduhr cells was sufficient to trigger the symmetrical tail movement unique to spontaneous slow...periodically beat their tails left and right in a symmetrical motion to move forward. This behavior is not ...behavior studies in action: Identifying neurons that trigger spontaneous swimming  Researchers at the Isacoff... zebrafish larvae to identify new neurons that trigger the CPG (central pattern generator) (Wyart et al... photostimulation in order to drive the full symmetrical response.  The function of Kolmer-Agduhr cells...

...plant hormones strigolactones By Fabienne Furt Current methods used to detect strigolactones (SLs) are costly...build and validate a ratiometric fluorescent biosensor based on the parasitic Striga hermonthica SL receptor...editing in organoid Biosensors for detecting strigolactones The CRISPR Corner New ready-to-use viral preps... catalytic activity.  As SLs are also known to trigger seed germination of parasitic plants, this approach...

...sequencing results match sequence information contributed by the depositor, published backbone sequences...additional verification experiments: Primer and restriction digest design Some regions of plasmids are difficult...present in the plasmid, we might need to perform a restriction digest to confirm the size of the plasmid and...including our most popular primers and certain restriction enzymes, but you will also be able to create ...annotations and design additional experiments like restriction digests. We’ll have a tutorial showing all of...Basic Molecular Biology Protocols Diagnostic Restriction Digest Protocol ...

...the list weekly. The Blue Flame Award, first distributed this year, serves to congratulate researchers...one plasmid deposited at Addgene that has been distributed more than 100 times. We hope these desktop trophies... or personnel to constantly prepare them for distribution. One of the major advantages to depositing materials...Laboratory in Norwich, UK: “We’re very pleased to distribute our plasmids via Addgene. Given the increasing...world. “It’s easy to imagine how hard it is to distribute thousands of plasmids across the world from the...has made it possible to eliminate geographical restrictions in life science research." Claim Your Award ...

...resource of over 100 entries is driven by the dedicated community of data-contributing researchers who use...This word cloud (using wordart.com) represents contributed data on brain injection site from the AAV data...recently used Addgene AAV tools, we invite you to contribute your data to grow this resource for the community...opportunity to cite the image to give proper attribution.  We hope that this provides a clear overview...to all of those dedicated scientists who have contributed to the AAV Data Hub. If you haven’t yet, we encourage...

...transport at the cell surface Article contributed by Marcy Patrick  Listen to the nanobody toolkit podcast...highly processive reverse transcriptase Article contributed by Will Arnold  Listen to the MarathonRT podcast...occupying a specific genomic locus: Caspex  Article contributed by Susanna Bachle  Listen to the Caspex podcast...occupying a specific genomic locus: C-BERST Article contributed by Klaus Wanisch  Listen to the C-BERST podcast...dCas9–APEX2 biotinylation at genomic elements by restricted spatial tagging), to confirm the composition ...

...pR6K ~15-20 R6K* C Stringent pACYC ~10 p15A B Relaxed pSC101 ~5 pSC101 C Stringent pBluescript ~300...of replication is referred to as "relaxed" or "stringent" depending on whether the ori is positively regulated... do you choose? Addgene Senior Scientist Marcy Patrick says researchers can ask themselves a few questions...copy number of a plasmid, other external factors contribute as well. These considerations are especially ...are temperature sensitive; others ori's can be "tricked" into amplifying more copies with the addition ...

... This post was contributed by guest blogger Courtney Price, the Education & Outreach Specialist for the...mission is to collect, preserve, reproduce and distribute seeds, DNA and other resources for Arabidopsis...100,000 samples to researchers and educators in 60 countries each year. Introducing Arabidopsis Arabidopsis...fluorescent lights. Other experiments require only seeds, petri dishes, and filter paper. Another benefit of using...learning. Whether visiting classrooms, hosting field trips, conducting teacher workshops, or staffing a booth...partnerships have grown to include local school districts, professional education associations, and local...

...This post was contributed by Alessia Armezzani, scientific communication manager at genOway. A few decades...Hegemann, 1991). These proteins control the flow of electric charges across cell membranes and maintain membrane...neurons have in common? Neuronal stimulations are triggered by the movement of ions across the axon's membrane... is reached and the neuron fires, sending the electrical signal down the axon (Lodish et al., 2000). Most...light activates channelrhodopsins that, in turn, trigger neural excitation, whereas yellow light activates...stimulations, they simultaneously recorded the electrical impulses of discrete subpopulations of this brain...Through a partnership with genOway, we are able to distribute materials containing FLEx technology. Read the...

...This post was contributed by guest blogger Katrina Armstrong, a Neurophysiology Msc Student at the University...communities. Many thanks to our guest blogger Katrina Armstrong. Katrina Armstrong is a Neurophysiology Msc Student...better (3). Brain Region  Optimal Serotype Striatum AAV5>AAV9>AAV8>AAV1>AAV6>AAV2 Hippocampus AAV9...will depend upon which section you’re looking at (striatum, hippocampus and cortex) within the mouse brain...serotonin in movement. Follow her on twitter @katrinaboards.   References 1. Russell, David W. "AAV vectors...

...intensity, excitation and emission spectra. The lab of Dimitri Deheyn recently identified a family of GFP proteins... that they can be imported to the mitochondrial matrix. Gottlieb’s lab has used an inducible MitoTimer...into Golden Gate entry vectors via traditional restriction enzyme cloning, TA cloning, or annealing of oligonucleotides...team at the Institute of Science and Technology Austria, decided to take a novel approach to control for...activity levels and limited sensitivity under the stringently low expression conditions necessary to avoid ...chemogenetic technologies After several years of distributing his Designer Receptors Exclusively Activated...cloning strategy, MoClo, which uses the Type IIS restriction enzymes BsaI and BpiI/BbsI to efficiently assemble...

...use restriction digest to screen your clones, but there are many other ways to choose restriction enzymes...blue-white screen. Image from Stefan Walkowski.   Restriction digest Another way to distinguish between colonies...inserts from those that do not is by using a restriction digest. The key is to be strategic about your...route, check out our protocol video on using restriction digests to analyze plasmids, which can help you... you visualize this process, or visit our restriction analysis blog post. Colony PCR Colony PCR can...

...construct for C. elegans gene knockdown Article contributed by Alyssa Cecchetelli  Listen to the C. elegans... labeling with TurboID and miniTurbo Article contributed by Eric Perkins  Listen to the TurboID and miniTurbo...compartments is via proteomics--the study of the distribution and interactions of proteins within these structures...small-molecule substrate to map the spatiotemporal distribution and interaction networks of specific proteins...Changing the way we detect viral outbreaks Article contributed by Izzy Mueller  Listen to the viral detection... reveals novel peptide ligand motifs Article contributed by Michael Lemieux  Listen to the SH3 domain ...