We narrowed to 297 results for: IRES

1. Degrading DNA with Cascade-Cas3

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   Blog Post

   ...part of the Class 1 CRISPR family, which means it requires multiple Cas effectors to carry out genomic editing...

2. Antibodies 101: Conventional vs Spectral Flow Cytometry

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   Blog Post

   ...a few aspects in which spectral flow cytometry requires special considerations. Conventional flow cytometry...

3. PhD Applications After COVID

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   ...Potential PIs Even if you’re applying to a school that requires its students to do rotations in different labs...

4. Plasmids 101: Knockout/Knock-In Plasmids

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   Blog Post

   ...conditional knockout. This experimental design requires two rounds of recombination – the first removes...

5. Google Forums Round Up: First Impressions of NgAgo

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   Blog Post

   ...Additionally, in vitro assembly of NgAgo/ssDNA requires incubation at 55 °C - a dangerous, non-physiological...

6. PITChing MMEJ as an Alternative Route for Gene Editing

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   ...end-joining)? MMEJ, a form of alternative end-joining, requires only very small homology regions (5-25 bp) for...

7. Which Fluorescent Protein Should I Use?

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   Blog Post

   ...many FPs (particularly those derived from GFP) requires oxygen. Therefore, these FPs cannot be used in...

8. Plasmids 101: Secondary Nanobody Toolbox

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   ...expressed in bacteria, which means their production requires animal sacrifice and precludes genetic engineering...

9. Transferable Skills Guide: Identifying Your Transferable Skills

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   Blog Post

   ...list all the skills that your current or past job requires day-to-day, and you’ll get a great snapshot of...

10. An Introduction to Adenovirus

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    ...sense of what AdVs are about – and we hope that it inspires further use of existing vectors, as well as research...

11. Getting to Know Expansion Microscopy

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    ...of - and ExM is no exception. First, expansion requires fixed samples. This limitation may be the both...

12. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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    Blog Post

    ...the frontiers of fluorescence microscopy often requires more photons. For example, moving from transient...

13. Plasmids 101: Colony PCR

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    ...Molecular cloning requires some method of screening colonies for the presence of an insert. Traditionally...

14. CRISPR Antimicrobials

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    Blog Post

    ...PMC4466736. 5. Ando, Hiroki, Sebastien Lemire, Diana P. Pires, and Timothy K. Lu. “Engineering Modular Viral Scaffolds...

15. Cpf1: A New Tool for CRISPR Genome Editing

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    ...domain. Cpf1 cleaves DNA in a staggered pattern and requires only one RNA rather than the two (tracrRNA and...

16. Plasmids 101: Protein tags

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    ...cytometry, but rarely used for purification as elution requires low pH poly His HHHHHH  0.8 Affinity and Purification...

17. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    ...affinity purification, in 2009. However, iChIP requires insertion of recognition sequences of an exogenous...

18. Analyzing CRISPR Editing Results with ICE from Synthego

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    Blog Post

    ...generating reliable results from CRISPR edit data requires the help of robust software tools. As a consequence...

19. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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    ...passages. Furthermore, working with Sendai virus requires stringent biosafety measures, thereby increasing...

20. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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    ... following injection into the bloodstream, but requires a high vector dose and is unable to reach all ...