...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Fully... CRISPR Cut CRISPR Plasmids: Cut Browse CRISPR...CRISPR plasmids that create double strand breaks in DNA. CRISPR... Fully functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location...enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity... . Browse, sort, or search the tables below for CRISPR plasmids designed to introduce a DSB. Plasmids ...

... Addgene! CRISPR Please refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology...like... Plant CRISPR resources Fluorescent Protein Guide Addgene Blog: Plant Biology CRISPR Cloning Transcription...Rice - Yinong Yang. The MoClo CRISPR/Cas Toolkit for Plants includes CRISPR/Cas nucleases, base editors,...technology or the Plant CRISPR Resources for a full selection of plasmids expressing different functional variants... gRNA backbones. A few examples of useful plant CRISPR resources include: A Multipurpose Toolkit enables...truncatula , wheat, and barley - Daniel Voytas. A CRISPR/Cas9 Toolkit for use in a variety of plant species...series of vectors and DNA modules for assembly of CRISPR/Cas9 constructs - Sylvestre Marillonnet and Johannes...

...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Catalytically...Activate CRISPR Plasmids: Activate Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick...CRISPR plasmids for activating or upregulating gene expression in various model organisms. CRISPR... CRISPR Activate...enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity... . Browse, sort, or search the tables below for CRISPR plasmids for transcriptional activation. Plasmids...

...Microbiology General CRISPR Resources Read our CRISPR Guide for a general introduction to CRISPR technology. Check...software. Addgene yeast CRISPR resources Bacterial CRISPR Resources The CRISPR technology was developed...synthetic biology. CRISPR Resources : Find essential background information on using CRISPR in your lab, specific... Check out our CRISPR reference page for CRISPR resources, such as depositor protocols and gRNA design...Find CRISPR tools for use in genome editing of bacteria. CRISPathBrick Multiplex Plasmid : CRISPR expression... specific CRISPR tools for genome editing in bacteria, and more. Synthetic Biology : Browse our deposited...editing is not always straightforward. Read our CRISPR Methods for Bacterial Genome Engineering blog post...

...Bargmann’s keynote speech and in the workshops on CRISPR techniques and new tools for conditional expression...of your gene of interest.  SapTrap Tool Kit for CRISPR Cas9 edits At Addgene, we have the entire SapTrap...toolkit and plasmid assembly line for the creation of CRISPR targeting vectors that each contains a guide RNA...Schwartz and Jorgensen, 2016). Using SapTrap, this CRISPR plasmid can be created in a single tube. You can...Jorgensen. "SapTrap, a toolkit for high-throughput CRISPR/Cas9 gene modification in Caenorhabditis elegans...Addgene blog Learn more about SapTrap Read about CRISPR/Cas9 in C. elegans Browse our Plasmids 101 blog...

...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... CRISPR Xenopus CRISPR Plasmids: Xenopus Browse...CRISPR plasmids that have been designed for use in Xenopus. CRISPR... Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation... The following CRISPR plasmids have been designed for use in Xenopus. Cut Fully functional Cas9 enzymes...selectable marker or cloning method. When using CRISPR, you will need to express both a Cas protein and...

...other CRISPR reporters Browse our CRISPR blog posts Resources on Addgene.org Browse the CRISPR guide...the genome, but they’re inefficient compared to CRISPR/Cas9 edits that rely on double strand DNA breaks...converted into BFP (blue fluorescent protein) upon CRISPR/Cas9 homology directed repair (HDR) (Glasser et...Conversion: A Versatile Assay for the Quantification of CRISPR/Cas9-mediated Genome Editing. Molecular Therapy...guide Find CRISPR plasmids for your research ...

...pooled CRISPR screens By Emily Bentley Building on their CROPseq method for optical pooled CRISPR screening...Multiplexed perturbation and decoding in pooled CRISPR screens Improved prime editor PE7 Antibodies to...developed a multiplexing approach to Cas9-based pooled CRISPR screens called CROPseq-multi (Walton et al. 2024...multiplexed perturbation and decoding in pooled CRISPR screens. bioRxiv 2024.03.17.585235; doi: https:...sequences for a range of applications, including CRISPR activation by dCas9-VPR, prime editing, and delivery...including plasmids, antibodies, viral vector preps, CRISPR tools, and more.  ... pegRNA. The Britt Adamson Lab recently used a CRISPRi screen to identify additional host factors impacting...

...discovery. CRISPR is a great example (the top requested plasmids from 2015 and 2016 were CRISPR plasmids...ubiquitin ligase, cellular senescence, autophagy, CRISPR, and so much more (find the full, searchable list...

...choice. For more help with CRISPR, read our recent blog post explaining how CRISPR is being used for bacterial...Check out our Genome Engineering Overview and our CRISPR guide to find out how to use genome editing techniques...

...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Two...CRISPR plasmids for precisely editing DNA bases without inducing a DNA break. CRISPR... CRISPR Base Edit CRISPR...CRISPR Plasmids: Base Edit Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit...AID-mediated mutagenesis (TAM) from the Chang lab and CRISPR-X from the Bassik lab , in which Cas9 is fused ...enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity... . Browse, sort, or search the tables below for CRISPR base editing plasmids. Plasmids are available for...

...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... CRISPR C. elegans CRISPR Plasmids: C. ...following CRISPR plasmids have been designed for use in C. elegans. Cut Fully functional CRISPR/Cas enzymes...CRISPR plasmids that have been designed for use in C. elegans. CRISPR.... elegans Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...selectable marker or cloning method. When using CRISPR, you will need to express both a Cas protein and...

...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...CRISPR plasmids that have been designed for use in Drosophila and other insects. CRISPR... CRISPR Drosophila CRISPR...CRISPR Plasmids: Drosophila Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime... The following CRISPR plasmids have been designed for use in Drosophila and other insects. Cut Fully functional... functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location based...Plasmid Gene/Insert Promoter PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand...

... CRISPR Qi Lab CRISPR Plasmids Qi Labs CRISPR Plasmids...Addgene You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts We have recently...CRISPR CRISPRi Cas9 gRNA plasmids from the Qi lab CRISPR...or human cells ( Qi et al. ). This CRISPR interfering system (CRISPRi), works independently of host cellular...uniqueness of CRISPRi, as compared to several recently published works on applying the wild-type CRISPR system...recently repurposed the bacterial immune system, CRISPR ( C lustered R egularly I nterspaced S hort P alindromic...genes. These plasmids are described in: Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific...

... in applying CRISPR for mouse genome editing. Post: Generating Mouse Models Using CRISPR/Cas9   Erik L...Tips for protein purification Replica plating Anti-CRISPR Retroviral vectors Career development for scientists...

...Collection CRISPR Please refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology...examples of useful zebrafish CRISPR resources can be found below: UFlip CRISPR targeted integration vectors...for Targeted Integration Using CRISPR/Cas9 - Jeffrey Essner Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression...Joung Lab CRISPR/Cas9-based conditional mutagenesis in zebrafish - Wenbiao Chen Lab A CRISPR/Cas9 Vector...modified CRISPR-Cas9 system - Shuo Lin Lab Re-purposed Ac/Ds transgenic system enabling CRISPR/dCas9-interference...for the laboratory use of zebrafish. CRISPRz - A database of CRISPR/Cas9 target sequences that have been...morpholinos, zinc finger nucleases, TALENS, and CRISPR/Cas9-based gene editing make the zebrafish a stalwart...

...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Base...CRISPR plasmids that have been designed for use in mammalian systems. CRISPR... CRISPR Mammalian Expression CRISPR...CRISPR Plasmids: Mammalian Expression The following CRISPR plasmids have been designed for use in mammalian...mammalian expression systems. Cut Fully functional CRISPR/Cas enzymes will introduce a double-strand break (...Promoter Selectable Marker PI Publication Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit...->G (or T->C on the opposite strand) mutations. CRISPR-X, a base-editing system from the Bassik lab, uses...

... CRISPR References & Information Genomic Deletions in Mammalian Cell Lines CRISPR: Protocol...Mammalian Cell Lines Other Addgene Resources CRISPR Plasmids CRISPR Guide Addgene Blog Posts Addgene is proud...regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) 9 system may be re-purposed.... Perform maxi-prep for each CRISPR/Cas9 construct. Transfecting CRISPRs into Cells of Interest NOTE: ...using CRISPR/Cas9 CRISPR...Orkin's and Daniel Bauer's labs details the use of CRISPR/Cas9 to create genomic deletions in mammalian cell...of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9. Bauer DE, Canver MC, Orkin SH. J. Vis. Exp...

...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Base... CRISPR Bacteria CRISPR Plasmids: Bacteria...following CRISPR plasmids have been designed for use in bacteria. Cut Fully functional CRISPR/Cas enzymes...CRISPR plasmids that have been designed for use in bacteria. CRISPR...Plasmid Gene/Insert Promoter PI Publication Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit...mutations. ID Plasmid Promoter PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand...Insert Promoter PI Publication RNA Targeting Type VI CRISPR systems, including the enzymes Cas13a/C2c2 and ...

...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... CRISPR Yeast CRISPR Plasmids: Yeast Browse ... following CRISPR plasmids have been designed for use in yeast. Cut Fully functional CRISPR/Cas enzymes...CRISPR plasmids that have been designed for use in yeast. CRISPR...Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation ... Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand...selectable marker or cloning method. When using CRISPR, you will need to express both a Cas protein and...