...in the educational sphere, it can range from researching an area, to writing an introduction that gives...mitosis and meiosis in biology. Lots of it is researching the different aspects of the subject you're writing... Keown, a staff writer at the non-profit Fred Hutchinson Cancer Research Center. And make sure to subscribe...

...constructing 14 luciferase donor Rluc8-Gα chimeras, four acceptor GFP2-Gγ chimeras, and two untagged Gβ constructs...or drug. In the inactive state, the luminescent chimeric protein Gα-Rluc8 and Gγ-GFP2 will be in close ...

...turn up the heat when there is a bad Northeastern chill. Check out Joanne's Reddit AMA  Few of us spend... in this country and we are quick to throw out chipped or imperfect equipment.  We can’t safely recycle...illustration above) and bribed the guys in the hospital machine shop with coffee and donuts to cut the pieces of...

...a smaller RNA tag, are less prone to photobleaching, and achieve better signal in vivo as compared to ...dCas9 fused to cytidine deaminases like APOBEC. To achieve this they created a lentiviral vector system that...

...donated to ABRC by the Arabidopsis research and teaching community through the TRAINED initiative (Translating...ordering from ABRC for the first time, we recommend reaching out to us at [email protected] for help setting ...professor? Whether you are looking to develop a new teaching resource or you want to introduce Arabidopsis ...

...instability-prone lentiviral vector plasmid in Escherichia coli Stbl3. 3 Biotech 3:61–70. https://doi.org...lentiviral expression vectors using reduced-genomeEscherichia coli. BioTechniques 43:466–470. https://doi.org...cloning strategy for efficient genome editing in Escherichia coli. Microb Cell Fact 19. https://doi.org/10.1186...

...including a mouse monoclonal (M2) as well as a chimera (rabbit). Myc The Myc tag is a 10 amino acid peptide...still the most prevalent clone used today! Goat, chicken, mouse, or rabbit? Addgene has you covered with...Addgene’s monoclonal antibodies (mouse, rabbit, and chicken) to this tag! GFP  GFP is more than a peptide –...

...PMCID: PMC1209583. Watanabe, Tsutomu, Chizuko Ogata, and Sachiko Sato. “EPISOME-MEDIATED TRANSFER OF DRUG...and Esther M. Lederberg. “Sex Compatibility in Escherichia Coli.” Genetics 37.6 (1952): 720–730. PubMed ...

...their structure or their interaction with AAVs (Schiller et al., 2018). Exosomal vesicles secreted from...behind these improvements. What have exosome AAVs achieved so far? Exo-AAVs have improved transduction profiles...PMID: 29296848. PubMed Central PMCID: PMC5728288. Schiller, Lara Timantra, et al. "Enhanced Production of...

...guides – including an excellent series of posts by Joachim Goedhart on the Addgene blog – outlining various...  Simply input these parameters and you’ll find matching FP suggestions. If you’re not sure exactly what...better way to store and represent within-study photobleaching comparisons.  We very much appreciate feedback...

... our purposes? In search of simple biological machines R bodies can repeatedly extend and contract...using R bodies: simple, force-generating protein machines that can extend and retract over many microns ...with a fluorescent protein remain functional. Attaching the fluorescent protein fusions allowed us to ...

...Refactoring and optimization of light-switchable Escherichia coli two-component systems. Schmidl SR, Sheth... at Addgene. Synthetic biology: engineering Escherichia coli to see light. Levskaya A, Chevalier AA, ...Multichromatic control of gene expression in Escherichia coli. Tabor JJ, Levskaya A, Voigt CA. J Mol Biol...

...can be affected by both autofluorescence and photobleaching. Researchers have long been interested in using... cells. As Nano-lanterns are not subject to photobleaching/phototoxicity, they successfully imaged multiple...applications. For long-term imaging, especially where photobleaching and phototoxicity are a concern, these constructs...

...samples, the rest will be put into a status we call “Archived.” This status allows us to store plasmids and ...These new processes have been instrumental in achieving our mission to help science move faster in the...Resources Page! References Fukushi S, Watanabe R, Taguchi F (2008) Pseudotyped Vesicular Stomatitis Virus...

...instead use a preexisting, conserved cellular machinery to process gRNAs. Hijacking tRNA processing to...plants. Yang was intrigued by the tRNA processing machinery present in almost every living organism. Precursor...PTG constructs in rice protoplasts. Compared to matching traditional gRNA-expressing constructs, transcript...

... Keown, a staff writer at the non-profit Fred Hutchinson Cancer Research Center. Susan Keown’s science...position at Fred Hutch because her skill set wasn’t matching up with the local job postings. “There were jobs...applied and I got it.” Science writing at Fred Hutchinson Cancer Research Center Work as a Science Writer...

...developing assays.  Protocol architecture The basic (very basic!) architecture of the indirect approach is...method relies on multiple secondary antibodies attaching to a single primary antibody, it is sometimes ...

...what Shoukhrat Mitalipov and his colleagues have achieved, and how the field will move forward from this...circumvent this problem, they co-injected CRISPR machinery with sperm into M phase oocytes, thus allowing...’s important to remember that the study hasn’t achieved any end goal not available with current techniques...

...GFP. The lab tested the base editors in vivo and achieved therapeutically relevant editing efficiencies ...heart, and skeletal muscle. The dosing required to achieve these efficiencies was also between 1013 - 1014...Ace absorbance increases and results in higher quenching of mScarlet emission. Figure 2: Ace-mScarlet...

...back into the infants. Almost all patients have achieved persistent immunological reconstitution with a...mutations in the gene RPE65. It is characterized by childhood onset of blindness and was thought to be untreatable...Improvement and decline in vision with gene therapy in childhood blindness." New England Journal of Medicine 372.20...