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Addgene
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  1. Viral Production at Addgene

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    Blog Post
    ... to AAV References Lock, M., Alvira, M. R., Chen, S.-J., & Wilson, J. M. (2014). Absolute determination...doi.org/10.1089/hgtb.2013.131 Wang, Y., Bergelson, S., & Feschenko, M. (2018). Determination of Lentiviral...
  2. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    Blog Post
    ... the genome to introduce the genetic modification(s) contained within it by selecting for Zeocin resistance...19416369. Kulakauskas, Saulius, Arvydas Lubys, and S. Dusko Ehrlich. "DNA restriction-modification systems...
  3. Tips for arabidopsis transformation

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    Blog Post
    ...transgenic plants in the process.     References 1. Emami, S., Yee, M. and Dinneny, J. R. (2013) ‘A robust family...24032037. PubMed Central PMCID: PMC3759027. 2 Harrison, S. J. et al. (2006) ‘A rapid and robust method of identifying...
  4. Course-Based Undergraduate Research Experiences (CUREs)

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    Blog Post
    ...References and Resources References Bangera, G., Brownell, S. 2014. Course-based undergraduate research experiences...Sciences Education. 15(4):ar55. Ma, Y., Mukherjee, S., Uzzi, B. 2020. Mentorship and protégé success in...
  5. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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    Blog Post
    ... Jul 27, 2020. CRISPR, and specifically Cas9 from S. pyogenes (SpCas9), is truly an exceptional genome... this paper at Addgene.  3. Gootenberg, Jonathan S., et al. "Nucleic acid detection with CRISPR-Cas13a...
  6. CRISPR 101: RNA Editing with Cas13

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    Blog Post
    ...identified that a S375A mutation in RESCUE (named RESCUE-S) offered ~76% on-target C-to-U editing while reducing...from this paper at Addgene. Gootenberg, Jonathan S., et al. "Nucleic acid detection with CRISPR-Cas13a...
  7. Lentiviral Vector Uses and Overview

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    Blog Post
    ... Kabbara N, Dalle B, Gourmel B, Socie G, Chrétien S, Cartier N, Aubourg P, Fischer A, Cornetta K, Galacteros...Beuzard Y, Gluckman E, Bushman F, Hacein-Bey-Abina S, Leboulch P. Nature. 2010 Sep 16;467(7313):318-22....
  8. A Career in Grants: How To Become a Grant Professional

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    Blog Post
    ...transferable. Use your experience and your passion(s) to guide you in building a grant career. Do you have... client or job will save you and the organization(s) you serve time and resources. 3. Get a mentor, find...
  9. Light Sheet Fluorescence Microscopy

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    Blog Post
    ... menschlichen und tierischen Praparaten. Leipzig: S. Hierzel.
 J.A Dent, A.G. Polson, M.W. Klymkowsky....1983–1995. Pubmed. K. Chung, J. Wallace, S.Y. Kim, S. Kalyanasundaram, A.S. Andalman, T.J. Davidson et ...
  10. Plasmids 101: Protein tags

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    Blog Post
    ... peptide-based proteosome inhibitors, glutathione S-transferase (GST), which can be fused to recombinant...Purification Very small size, rarely affects function S-tag KETAAAKFERQHMDS  1.8 Solubility and Affinity Abundance...
  11. Distribution to Industry

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    Collection
    ...between the recipient and the depositing institution(s). It governs the transfer of biological materials ...
  12. Synthetic Biology - Overview

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    Collection
    ...cpFRET Kit Rinehart Recombinant Phosphoprotein Kit S. cerevisiae Advanced Gateway Destination Vectors TAL...
  13. Retrograde AAV viral preps

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    ...50363 AAV phSyn1(S)-DreO-bGHpA Syn Dre expression Recombinases Zeng 51669 AAV phSyn1(S)-FlpO-bGHpA Syn ...
  14. Plasmids 101: Modular Cloning Applications and Kits

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    Blog Post
    ... then the MoClo YTK Kit specifically designed for S. cerevisiae is at your service (Lee et al., 2015)....al., 2017). In addition to additional elements for S. cerevisiae chromosomal integration and gene expression...//doi.org/10.1021/acssynbio.9b00322. Kim, Matthew S., et al. Degron-Based bioPROTACs for Controlling Signaling...
  15. Antibodies 101: Introduction to Immunofluorescence

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    Blog Post
    ...References and resources References Im K, Mareninov S, Diaz MFP, Yong WH (2018) An Introduction to Performing...fluorescence-microscopy/   Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, Rodriguez...
Showing: 221 - 240 of 413 results