...and carry the metallothionein promoter (e.g. pMT-puro). Worm vectors There are several approaches to studying...

...Resource List . NeuroMab/Trimmer Lab Recombinant mAb Plasmid Collection . The NeuroMab Facility and James...supports the development of biomolecular tools for neuroscience research. Browse the BRAIN Initiative collection...Brain Research through Advancing Innovative Neurotechnologies® (BRAIN) Initiative aims to revolutionize...recombinase-dependent dTomato expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element...

...audiences. She focuses at Science on developmental neurobiology and plant sciences, viewed through the lens ...

...is effective in sensitive cells such as living neurons, and can be fused to other fluorescent proteins...

...predicted cell type (e.g., axon-like projections for neuron predicted differentiation), and 15/17 morphologically...

...length GFP and converted to recombinant form by the NeuroMab Facility Isotype IgG2a Differences:  Distinct...

... if you need to. Additionally, they have an IRES-Puro marker to facilitate stable cell clone selection...

... (GEVIs) change fluorescence in real time when neurons or muscle cells transmit electrical information...

...immunogenicity prediction of therapeutic proteins. European journal of pharmaceutics and biopharmaceutics ...

...disease mutation in basal forebrain cholinergic neurons derived from iPSCs. From Rohn et al. 2018; see ...

...Using translation inhibitors cycloheximide and puromycin, Halstead et al. verified that translation is ...

...R-body producing recombinant E. coli on paramecia. European Journal of Protistology 48, 290–296 (2012). PubMed...

... forget that this technique isn’t limited to neuroscience. In fact, precise light-based control of biological...

...80S; inhibits chain elongation. 50-500 ug/mL Puromycin pac HeLa, 293HEK, Jurkat T cells Inhibits protein...

...protein) in striatal Pvalb+ neurons. Note strong cortical Pvalb and L5 ET neuron expression. Jonathan Ting...collection of Armamentarium tools will aid neuroscientists in studying circuits in laboratory animals ...technologies have the potential to transform neuroscience research by bringing the precision of molecular...Expression of CoChR-EGFP in striatal cholinergic neurons Jonathan Ting Viviana Gradinaru 214862-PHPeB AiP14825...Expression of iCre(R297T) in striatal medium spiny neurons Jonathan Ting Viviana Gradinaru 214858-PHPeB AiP14496...separate nuclear FLAG epitope in striatal cholinergic neurons Jonathan Ting Viviana Gradinaru 214857-PHPeB AiP14361...jGCaMP8s in striatal indirect pathway medium spiny neurons (D2 MSNs) Jonathan Ting Viviana Gradinaru 214856...

...functionally distinct interneurons in mice, non-human primates and humans. Nat Neurosci. 2020 Dec;23(12):1629...targeting and manipulating interneurons across vertebrate species. Nat Neurosci. 2016 Oct 31. Gordon Fishell...Dopamine Ultrafast neuronal imaging of dopamine dynamics (dLight1.1-1.5) Ultrafast neuronal imaging of dopamine...for glutamatergic neurotransmission Targeted sensors for glutamatergic neurotransmission. eLife. 2023 Jan...mouse hippocampal neurons. eLife. 2020 Jun 9;9. pii: 56469. Edwin Chapman Neuropeptides GRAB biosensors ... orexin neuropeptides A genetically encoded sensor for in vivo imaging of orexin neuropeptides. Nat Methods...expression in neurons A genetically encoded sensor for measuring serotonin dynamics. Nat Neurosci. 2021 Apr...

...)-2A-Puro (PX462) V2.0 62987 Mammalian BbsI yes, nick S. pyogenes Puro Zhang pSpCas9(BB)-2A-Puro (PX459...pyogenes Puro, TagBFP Yusa lentiGuide-Puro 52963 Mammalian/Lentiviral BsmBI none S. pyogenes Puro Zhang ...pyogenes Puro Moffat lenti sgRNA(MS2)_puro backbone 73795 Mammalian/Lentiviral U6 none S. pyogenes Puro Zhang...Lentiviral BsmBI yes, cut S. pyogenes Puro Zhang pKLV-U6gRNA(BbsI)-PGKpuro2ABFP 50946 Mammalian/Lentiviral BbsI...Ebert pLKO.1-puro U6 sgRNA BfuAI stuffer 50920 Mammalian/Lentiviral BfuAI none S. pyogenes Puro Wolfe pL-...pyogenes Puro Ebert pL-CRISPR.EFS.PAC 57828 Mammalian/Lentiviral BsmBI yes, cut S. pyogenes Puro Ebert pL-CRISPR.SFFV.PAC...pLenti SpBsmBI sgRNA Puro 62207 Mammalian/Lentiviral BsmBI none S. pyogenes Puro Maehr pKLV-U6gRNA-EF(...

...Fibroblasts Neurons Lentiviral Mouse Direct conversion of fibroblasts to functional neurons by defined ...Dopaminergic Neurons Lentiviral Human Direct conversion of human fibroblasts to dopaminergic neurons. Proc Natl...Fibroblasts Neurons Lentiviral Human MicroRNA-mediated conversion of human fibroblasts to neurons. Nature....Fibroblasts Cholinergic Neurons Lentiviral Human Small molecules enable neurogenin 2 to efficiently convert...cholinergic neurons. Nat Commun. 2013;4:2183. Zhang Fetal Lung Fibroblasts Cholinergic Neurons Lentiviral...molecules enable neurogenin 2 to efficiently convert human fibroblasts into cholinergic neurons. Nat Commun...Fibroblasts Medium Spiny Neurons Lentiviral Human Generation of Human Striatal Neurons by MicroRNA-Dependent...

... AAV-mediated delivery to iPS-derived cortical neurons, dCasRx-mediated exon skipping improved the ratio...

...Richter MF, Zhao KT, Eton E, Lapinaite A, Newby GA, Thuronyi BW, Wilson C, Koblan LW, Zeng J, Bauer DE, Doudna...