We narrowed to 564 results for: uros

...and Memory Formation. Neuron 79, 6 P1086-1093. https://doi.org/10.1016/j.neuron.2013.08.032.  Morita S...lentiviral backbones expressing blasticidin or puromycin resistance genes.  It’s that simple! Good luck...

... in Frontiers in Neuroanatomy and summarized some considerations for precise neuronal targeting using ...Considerations For Precise Neuronal Targeting Using AAV Technologies Using AAV for neuronal tracing Resources...

...Recombinant Antibodies in Basic Neuroscience Research. Curr Protoc Neurosci. 2020 Dec;94(1):e106. doi: 10.1002...PMC7665837. L'Abbé D, Bisson L, Gervais C, Grazzini E, Durocher Y. Transient Gene Expression in Suspension HEK293...

...Rochester Medical Center! Patrick is equal parts neuroscientist, writer, and extreme sports enthusiast. He ...He recently obtained his Ph.D. in neuroscience from the University of Rochester Medical Center, where he...biology techniques to study inflammation and neurodegeneration. He now spends his time learning and writing...

...Chemical Synapses in the Brain. Neuron 68:843–856. https://doi.org/10.1016/j.neuron.2010.11.021  Gambarotto D...helped researchers gain a deeper understanding of neuronal synapses, centrosomes, parasites, and more (Bertiaux...

...ion-based communication is not just for neurons. Non-neuronal cells do this too. It's just that for non-neural...you hear the phrase “action potential?” Firing neurons are likely the first thing that comes to mind. ...exhibits an inactivation maximum of 590 nm light. Aurora and Phobos optogenetic tools - A collection of ...

...slow-growing cells, particularly mammalian cells like neurons, may not replicate during the course of your experiment...shared by bacteria and eukaryotes. For example, puromycin inhibits protein synthesis across kingdoms by ...targeting a shared aspect of ribosomal function. A puromycin resistance gene on your vector can protect both...

...Technologies and Methods for Targeted Neuronal Manipulation. Frontiers in Neuroanatomy, 13. https://www.frontiersin.org...for Adeno-associated viral vector production. Neuromethods, 195, 77–101. https://doi.org/10.1007/978-1-...

...experimental systems. They demonstrated LOV-Turbo in neurons, in pulse-chase experiments, and more. Find LOV-Turbo...element (TRE) or the cFos promoter. For example, neurons treated with a phorbol ester activator gave fluorescent...Images of iPAK4 fibers in cultured rat hippocampal neurons. HaloTag dyes are switched from yellow to red at...

...in green monkeys and cultured human neurons, with broader neuronal expression than AAV9. This new capsid...https://doi.org/10.21203/rs.3.rs-3188559/v1.  Neurogenetic alchemy: CAP-Mac unleashes the secrets of primate...-brain barrier and delivering a transgene, with neuron bias observed in young macaques and vascular bias...

...Brain Research Through Advancing Innovative Neurotechnologies (BRAIN) Initiative Cell Census Network, the... Fig. 1: Antibody production methods    NeuroMab and others have an extensive library of monoclonal...mouse tissue and compare the IHC results to both NeuroMab and the Allen Brain Atlas. The comparison with...intuitive guide for checking their staining and neuroanatomy without the fear of referencing a wrong section...

...Since the pioneering days of Golgi and Cajal, neuroscientists have developed and refined many tools to tease...understand the complex interworking of the 86 billion neurons in the human brain. But studying synaptic connections...generally very small, and can contain different neurotransmitters. Using genetically encoded fluorescent proteins...inhibitory synapses, respectively. Once expressed in the neuron, the FingRs bind to the endogenous synaptic proteins...

... expression.  Cloning and expressing RNAi pMKO.1 puro (Plasmid #8452) RNA interference (RNAi) is an RNA-mediated...Vectors    Expressing your gene of interest pLenti-puro (Plasmid #39481) You can clone your gene of interest....1 vector can be found here. Cre-Lox technology Puro.Cre empty vector (Plasmid #17408)   This is a lentiviral...Tyler Jacks. It carries CRE recombinase and a puromycin resistance cassette. No time to produce virus...

...expressing validated antibodies for neuroscience research (see the NeuroMab Collection for details). The following..., I wanted to find a specific plasmid from the NeuroMab collection for an antibody against PARIS, a zinc...zinc finger protein involved in neurodegeneration in Parkinson’s disease. Knowing the primary investigator...

... rare genetic eye disease, Leber’s congenital amaurosis. This is the first of what I, scientists, doctors...childhood-onset form of blindness name Leber’s Congenital Amaurosis (LCA). Shortly after the cloning of the RPE65 ...efficiently transduce various types of retinal neurons. Nowadays a toolbox of naturally occurring and ...muscular dystrophy, spinal muscular atrophy, neurodegenerative diseases and metabolic diseases are also underway...

...lab develops iPSC-based models of neurodegenerative and neurovascular diseases. He worked on the described... Dominik Paquet is currently a Professor for Neurobiology at the Institute for Stroke and Dementia Research...

...-dCas9-p300 Core, as well as pLV-dCas9-p300-P2A-PuroR for lentiviral expression. Figure 1: dCas9... deposited pdCas9-DNMT3A-EGFP and pdCas9-DNMT3A-PuroR for targeted cytosine methylation in mammalian cells... cells. Co-expression markers EGFP and PuroR enable sorting and selection of transduced cells. Grant Challen...

...marker such as puromycin, you can (also) select for the positive colonies by puromycin selection. You ...transfected cells (for example, plasmid with a puromycin resistance cassette, such as PX459). After you...

...iPSCs) to take on a “neuron-like” phenotype when targeting genes involved in neurogenesis, which had proven...EGFP, mCherry, iRFP, tdTomato, luciferase, LacZ, puromycin or CreERT2 can be combined with custom-cloned ...a separate reporter gene (EGFP, iRFP, IFP1.4, puromycin, neomycin or luciferase) to create a final lentiviral... voltage indicator for use in the detection of neuronal action potentials. The Cohen lab wished to improve...the genome. In this work, they use AAVS1_Puro_PGK1_3xFLAG_Twin_Strep and nuclease driven recombination ...

...effective at editing cytosines preceded by guanine (Thuronyi et al., 2019). Following the development of adenine...high product purity and low off-target editing Thuronyi et al., 2019 evoAPOBEC1-BE4max   Improved editing... K. T., Eton, E., Lapinaite, A., Newby, G. A., Thuronyi, B. W., Wilson, C., Koblan, L. W., Zeng, J., Bauer...891. https://doi.org/10.1038/s41587-020-0453-z Thuronyi, B. W., Koblan, L. W., Levy, J. M., Yeh, W.-H....