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We narrowed to 281 results for: nif

Showing: 201 - 220 of 281 results

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Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

Type

Blog Post

...delivery of split base editors Article contributed by Jennifer Tsang AAVs are a great gene therapy delivery mechanism...
RaPID Detection of RNA-protein Interactions

Type

Blog Post

...datasets. Applications of RaPID RaPID sounds like a nifty technique, but what kinds of questions can it help...
Antibodies 101: Secondary Antibodies

Type

Blog Post

...which means that indirect methods are often significantly longer than direct ones. Even though the secondary...
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

Type

Blog Post

...plasma membrane. Note that the use of the HA tag significantly improves the transport of hM4Di to the plasma...
RANbodies: Reporter Nanobody Fusions

Type

Blog Post

...antibody-containing hybridoma supernatant is only $40, significantly cheaper than commercially available antibodies...
Human Germline Editing Using CRISPR

Type

Blog Post

...biologists and non-biologists alike. Given the public significance of this research, we have the obligation to ...
Addgene's Tips for Plasmid Quality Control

Type

Blog Post

...always be derived from a single colony to ensure uniformity of the culture. Sequencing directly from the ...
Career Coaching for Scientists: Why and Where Do I Find One?

Type

Blog Post

... for PhDs and postdocs posted by Toronto-based Jennifer Polk of From PhD to Life. She runs great tweet...
5 Steps to Writing Grant Proposals for Grad Students

Type

Blog Post

...strategy The Research Strategy highlights i) the significance and innovation of the project and ii) how you...
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

Type

Blog Post

... May 23, 2017 and last updated Jul 23, 2020 by Jennifer Tsang. CRISPR-Cas technology is constantly evolving...
Viral Vectors 101: Calcium Sensors

Type

Blog Post

...delivered using viral vectors! One of the most significant advantages of viral delivery is the potential...
High Complexity Golden Gate Assembly with NEB’s DAD

Type

Blog Post

...of a lac operon dropped to 49% fidelity, with significant lot-to-lot variation. Additionally, this assembly...
Popular Retroviral Vectors and Their Uses in Scientific Research

Type

Blog Post

...ecotropic or amphotropic MuLE lentivirus provides significant genetic power and allows high throughput genetic...
Adenoviral Vector Production and Troubleshooting

Type

Blog Post

...years since its discovery, researchers have done significant work to improve the Adv genome’s utility in research...
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

Type

Blog Post

...Addgene, each part of this new process involves a significant amount of QC. First, we perform Picogreen quantification...
Hot Plasmids and Viral Preps - January 2021

Type

Blog Post

...doi.org/10.1126/sciadv.abb7438 The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR...
Experimenting in an Open Source lab: from CRISPR to Cats

Type

Blog Post

...interested parties can do research. This interest manifested itself in the creation of Hackerspaces and Makerspaces...
Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

Type

Blog Post

...-Crick base pair substrates, it demonstrates significant activity on some mismatch-containing pairings...
Twenty Years of Addgene Sharing: CRISPR

Type

Blog Post

...other countries, from Japan to Australia, are significant depositors and requestors. CRISPR tools are developed...
Viral Vectors 101: Voltage Indicators

Type

Blog Post

...comparable to its one component counterpart and is significantly brighter and available with red-shifted fluorophores...

Showing: 201 - 220 of 281 results

Results per page:

Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

RaPID Detection of RNA-protein Interactions

Antibodies 101: Secondary Antibodies

Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

RANbodies: Reporter Nanobody Fusions

Human Germline Editing Using CRISPR

Addgene's Tips for Plasmid Quality Control

Career Coaching for Scientists: Why and Where Do I Find One?

5 Steps to Writing Grant Proposals for Grad Students

CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

Viral Vectors 101: Calcium Sensors

High Complexity Golden Gate Assembly with NEB’s DAD

Popular Retroviral Vectors and Their Uses in Scientific Research

Adenoviral Vector Production and Troubleshooting

Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

Hot Plasmids and Viral Preps - January 2021

Experimenting in an Open Source lab: from CRISPR to Cats

Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

Twenty Years of Addgene Sharing: CRISPR

Viral Vectors 101: Voltage Indicators

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