...At Addgene, it is our mission to make it easy for you to share plasmids. To achieve this goal, we will...and perform standard quality control but will not make the plasmids available until you update us with ...by sequencing. Upload any support files that will make it easier for scientists to best utilize your plasmids...it appears in the Entrez Gene list, as this will make it easier for other scientists to find information...and Mammalian Expression). This information will make it easier for scientists to find your plasmid by...Please review all of the information on this page to make sure it is correct. You can easily update the information...

...action. Instead of rushing in, you take steps to make it more likely you’ll get your tasks done correctly...to employers because they save time and money and make employees more productive. Careers where you’ll ...applicants compared to the number of available roles). Make your resume stand out Before you begin to apply ... need to think of other aspects of yourself that make you stand out. For every role you need to show that...Grad Student to Entrepreneur Learn to Network and Make the Contacts for Your Dream Job Resources on Addgene.org...

...the readability of your writing. It makes suggestions on how to make your writing less complex. While I...’s a list of tools and resources that might help make writing a little easier. Writing schedule tools ... should accept all of the suggestions Hemmngway makes (it wasn’t designed for technical writing), it’s...and you can share your library with others which makes it easier to work with collaborators. Zotero is ...

...well-understood standardized terms makes it a lot easier for organizations to make policy decisions on research...positive feedback on “our” MTAs, which sometimes makes people think our MTAs are different than other institutes.... Setting clear expectations from the beginning makes the sharing process smoother, more consistent, and...Stopwatch award and seek feedback as to how we can make our process easier for others.  If you’ve still ...

...working toward a solution. At the end of the meeting, make sure to thank everyone for their participation and...through a meeting with no idea why you are there. Make the most of everyone’s time by considering the level...grows, so will the length of your group meetings. Make sure to keep them manageable. At Addgene, teams ...last meeting. Use some positive peer pressure to make sure everyone completes their assigned items to ...like to send around a Google doc where people can make comments. At meetings, we just focus on the parts...

...documentation helps make sure everyone is on the same page. Effective delegation allows you to make the best use...expected to DO all the work – she is expected to make sure the work gets done and done well. Involving...for failure by delegating is a bad idea. How do I make sure it goes well? As with most managerial responsibilities...mistakes will be made that could have been prevented.  Make sure everyone understands that no question is “dumb...

...goals. You must understand your own style before making this choice.  How big is the lab? While larger ... the position. Am I choosing the right mentor? Make sure your advisors will be good role models and ...non-academic career path?  If you are considering this, make sure to choose an advisor that supports both paths...is one case where the topic of your research can make your transition easier – choose a lab that works...always be in the zone, but even brief stays there can make all the difference. Choose a lab that brings on ...

...Gel issues usually come down to voltage and time. Make sure to load an appropriate protein ladder on your...issue and can’t figure out what’s causing it, try making fresh running buffer. Old or improperly made running...in our Technical Design of a Western Blot post to make sure you have the optimal gel for your experiment...transfer. If you are expecting low amounts of protein, make sure your reversible staining method of choice is... fairly quickly, even at 4 °C, so when in doubt, make up a fresh batch.  Antibodies We always recommend...the solution used to expose them. You’ll need to make sure the sensitivity of your exposure solution matches... amounts directly, stripping and reprobing. Just make sure to go from least to most abundant protein if...

...the improvements we’ve implemented and how they’ll make it easier for you to find the plasmids you need....SnapGene powered plasmid maps at Addgene  Three factors make our SnapGene powered plasmid maps far easier to ...identities of most features labeled directly on them making them much easier to quickly identify. Improved ... to paste directly into your notebook. This will make it easier for you to share plasmid information with...their functions. We hope that these updates will make it much easier to browse the repository and quickly...

...transgene has integrated and make genetic crosses with balancer chromosomes to make genetically stable fly ...needs. If YFG is thrillingly new, you might have to make a transgenic fly in order to study it. The process...to worry! In flies, phenotypic selection markers make it easy to spot flies with the appropriate transgene...important to note so that you know what to look for. Making your transgenic fly and downstream experiments ...

...or a G to A change on the opposite strand). ABEs make an A to G change (or a T to C change on the opposite..., from David Liu’s lab has allowed scientists to make all 12 possible base-to-base changes. How do base... A Cas nickase or Cas fused to a deaminase that makes the edit A gRNA targeting Cas to a specific locus...efficiency for mammalian edits is to ensure the editors make it into the nucleus and that they are expressed ...achieve 98-99% target modification in primary T cells making them a promising tool for cell therapy applications..., Liquori AJ, Murray R, Packer MS, Rinaldi C, Slaymaker IM, Yen J, Young LE, Ciaramella G (2020) Directed...

...the development of a simple and scalable means of making targeted genetic edits. Cas9 is a DNA binding protein...Traditional Cas9 regulators In addition to its utility in making targeted modifications to DNA, Cas9 can be reprogrammed...modifying DNA and of modulating gene expression makes it an invaluable tool in the interrogation of gene...potent Cas9-based transcriptional regulator without making any functional changes to Cas9. For instance, when...been found to retain some ability to induce Cas9 to make edits (AC, WLC, and JQ, unpublished results). An...

...gelsolin, but any nanobody sequence could be used to make a RANbody. RANbodies are expressed in 293T cells...cells due to their IgGκ signal peptide sequence, making them easy to harvest from cell culture media. Their...staining GFP, RFP, H2A2B   Are you ready to start making your own RANbodies? Great! All of the RANbody plasmids... RANbodies. If you know of a nanobody that would make a useful RANbody, consider leaving a link to its...immunodetection If you enjoy DIY lab projects, consider making you own DNA ladders with these plasmids from Penn...

...antibodies in a single experiment then you will need to make sure that you can easily distinguish each different...before you will not only have peace of mind as you make your purchase but also someone to turn to for advice...epitope target, source, and other information to make sure you’re not trying to choose between the same...different names.  If all else is equal, feel free to make the decision by price point, shipping time, or other...experimental considerations - no other factors can make up for an antibody that doesn't work for your application...

...support for your ideas, invite others into decision making processes, and even interview better! As a scientist...happened to me.) They happen to everyone! Everybody makes mistakes, encounters technical difficulties, and...deep breaths. Then, identify the specific things making you nervous–audience attention? fear of messing...messing up?–and use the three tips above to make a strategy that can help. Even something as simple as telling...learned this skill in its most difficult form, which makes it the perfect transferable skill. Almost every ...

... In this first example to the right (# this will make you sad),  someone has put in a request for a particular...hand, we can go back and optimize our procedure to make the whole process work more efficiently. Get on ... Enjoy your happy lab staff. Barcoded materials make everyone's life easier, the time and capital cost...case, the physical considerations of the laboratory make object composition a smarter choice than inheritance...anything other than Python or MATLAB. Choosing Python makes it easy to spin up a read-only Jupyter notebook ...

...verify the identity and quality of the plasmids we make available and provide some advice on the steps you... information in a binder or digital notebook and make sure you note the freezer location of every plasmid...typical reaction, or about 250 amino acids worth, so make sure the feature you want to verify is within this...perform Sanger sequencing from glycerol stocks, so make sure to select the appropriate service and read ... a large number of sequencing reactions, you can make your life much easier by selecting the right primer...

...process. Again, thanks to our partners at seqWell we make use of a pipeline that takes the raw data from each... is useful, but not 100% complete, we will still make this data available as a partial sequence with the... load it on to our new MiSeq and set up the run making sure to note the appropriate barcode for each sample...described by Joung et al. 2017, which we hope to make available as part of our OpenBio Repository sometime...K, Sanders E, Tasissa M, Kostman M, Tillgren M, Makana Hanley L, Mueller I, Mitsopoulos A, Fan M (2020...

...instructions. If your enzyme did not cut, check to make sure that it isn't methylation sensitive . ...

...of this?” are all very valid questions, and often make an already complicated process even more difficult...program is the best fit for you and your goals. Making a List of Schools Creating a list of schools is... to highlight in the interview is another way to make the virtual environment more comfortable and help...presentation. It can help soothe your nerves and make sure you cover everything you need to during the...COVID-19, financial and logistical obstacles could make touring potential programs and universities stressful...