We narrowed to 326 results for: cloning

...commonly used in molecular cloning techniques, such as PCR or restriction cloning . It is also used to quickly...typically involves ∼500 ng of DNA, while molecular cloning often requires 1 µg of DNA. The total reaction ...sufficient. For digests with >1 µg of DNA used for cloning, it is recommended that you digest for at least...finding an enzyme that cuts your vector's multiple cloning site (MCS), but does not cut your insert, you could...prevent re-circularization of the vector if you are cloning in an insert. CIP (calf alkaline phosphatase) or...

...degraded. Cloning Grade DNA (high quality/low quantity) – You can usually complete a cloning experiment...

... Complexity Golden Gate Assembly Standards Kit Cloning Eric Cantor Kit for using comprehensive end-joining...Expression Sylvestre Marillonnet 95 plasmids for cloning and assembling standardized parts to create eukaryotic...

... Then, your desired DNA to be integrated can be cloned into the vector that you have selected. The nucleic...

...CDF) D Relaxed This table defines common cloning vectors, their copy number, ori, and incompatibility...

...research Browse protocols for molecular biology, cloning, and more ...

...basic lab techniques Plasmid Land: The plasmid cloning game ...

...procedures. Preventable errors in the design of cloning strategies set experiments back days or even weeks...

...of non-plasmid DNA or heterogeneous and/or mixed clones present in the original sample. At seqWell, we ...

... To use this SEC-based system, first design and clone 500-700 bp homology arms for your gene of interest...

...into your functional experiments! Whether you’ve cloned the plasmid yourself or obtained it from a colleague...

...after 6 years in the business of western blots and cloning, I decided to pack it in. I had just started month...

...sets of plasmids intended for use together (e.g. cloning and expression systems) and therefore supplied ...editing in C. elegans via a CRISPR-Cas9 system. Cloning-free CRISPR for C. elegans , which uses purified...

...of gRNA (e.g., pegRNA, epegRNA, nicking sgRNA), cloning enzyme, selectable marker, and whether the plasmid...Plasmid Expression System Promoter Guide RNA Type Cloning Enzyme Co-expressed Cas9 Selection PI 132777 pU6...

... Protocols Gibson Cloning Gibson Assembly Cloning You may also like... Cloning Methods...Assembly Cloning! Protocols...biologists now use this method extensively. Why Gibson Cloning? No need for specific restriction sites. Join almost...

...Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation...

...Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation...

...Backbones Find backbones or constructs to use for cloning to control expression of your gene of interest....EZ-Tet-pLKO-Puro Lentiviral Tet-On plasmid for easier cloning and design of inducible shRNA expression, with ...Sleeping Beauty transposon system with luciferase in cloning site. See article ( Kowarz et al., 2015 ) for additional... for inducible expression; insert with Gateway cloning and puromycin selection rtTA-Advanced Tight TRE...

...Additional Resources on Addgene.org  Yeast-mediated cloning CRISPR plasmids: Yeast Yeast Synthetic Biology...

...truncated versions of SARS-CoV-2 S (spike protein) cloned into a CMV driven expression vector are also available...