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We narrowed to 331 results for: cloning

Showing: 201 - 220 of 331 results

  1. Lentivirus Plasmids

    Type
    Collection
    ....1 – TRC cloning plasmid 3rd U6-driven shRNA empty plasmid; includes a stuffer for easy cloning Root 14748...shRNA. See article for detailed information about cloning & additional plasmids. Aebischer and Trono 11795...cassette (promoter and gene of interest) can be cloned into the plasmid. Nolan 12257 pWPXL 2nd EF-1alpha... the gene of interest, also can express shRNA (cloning H1-shRNA cassettes into the unique SnaBI site in...

  5. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...my lab we do a lot of genetic construction and cloning experiments. Not all of our lab enzymes are made...

  7. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... for Cloning 67720 Worm NotI/BamHI none S. pyogenes Colaiacovo pRB17 52527 Fly blunt end cloning none ...or nick. Selection , such as Puromycin or EGFP Cloning enzyme used for insertion of your gRNA sequence... by the Doudna Lab . Plasmid Expression System Cloning Enzyme Co-expressed Cas9 Cas9 System Selection ...BbsI yes, cut S. pyogenes Puro Zhang sgRNA(MS2) cloning backbone 61424 Mammalian BbsI none S. pyogenes ... U6 yes, cut (enhanced) S. pyogenes Zhang gRNA Cloning Vector Bbs I ver. 2 85586 Mammalian BbsI None S...nickase expression cassette using the Golden Gate cloning method. A separate accessory pack is available ...expressed from independent promoters. sgRNAs are cloned into separate vectors and then assembled into one...

  8. Open Enzyme Collection

    Type
    Collection
    ...Jennifer Molloy Lab Plasmids Blog: Golden Gate Cloning (Link opens in a new window) The Open Enzyme Collection...collection include polymerase chain reaction (PCR), cloning, isothermal amplification, reverse transcription...the basis of manufacturing their own enzymes by cloning them into an expression vector of their choice ...not functional by themselves. Researchers should clone these enzymes into an expression vector of their...

  12. Hot Plasmids - February 2022

    Type
    Blog Post
    ...popularity of FLAG and HA tags, you may not even need to clone new constructs to label your favorite protein! While...

  15. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...removal – in conventional mouse targeting, targeted clones are selected for using a resistance marker; however...

  16. Plan Your Experiment

    Type
    Collection
    ...using standard restriction-ligation cloning. However, the exact cloning strategy will depend on the gRNA ...including selecting the edit type, designing and cloning a gRNA, delivering Cas and the gRNA, and validating...Introduction Select Genetic Manipulation Design and Clone gRNA Select Expression and Delivery Validate Genetic...the multi-plasmid SAM system Activate Design and Clone Your gRNA Achieving your desired edit comes down...few factors to keep in mind when designing and cloning your gRNAs. Select Genetic Element to be Targeted... about how to design your gRNA . Synthesize and Clone Desired gRNAs Once your target sequences are selected...selected, it’s time to design your gRNA oligos and clone them into your desired vector. In many cases, you...

  18. CRISPR Plasmids - Xenopus

    Type
    Collection
    ... based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express...-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9...

  19. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ... based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express...-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9...

  20. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...promoter. WPRE has been removed to increase the cloning capacity. pCDH-CB-IRES-copGFP-T2A-Puro 72299 Express...well as the Cre and Flp recombinases. Genes can be cloned into these transfer vectors using shuttle vectors...Addgene #73345) is uniquely suited to simplify the cloning process as it can be used in blue–white screening...
Showing: 201 - 220 of 331 results