...into your functional experiments! Whether you’ve cloned the plasmid yourself or obtained it from a colleague...

...after 6 years in the business of western blots and cloning, I decided to pack it in. I had just started month...

...Pathway Clone Collection 2.0 : Set of vectors from the Esposito Lab for use with the Gateway® Cloning Platform...

...The original green fluorescent protein (GFP) was cloned in 1992 ( Prasher et al., Gene, 1992 ), and since...506 517 93 5.7 10 min Monomer pCS2+mNeonGreen-C Cloning Vector - Mammalian Expression Jump to Top Yellow...529 59 5.7 Monomer (A206K) pcDNA3 mCitrine LIC cloning vector (6C) - Mammalian Expression pCS2+8CmCitrine.../Xenopus/C.elegans/Sea urchin pET mCitrine LIC cloning vector (u-mCitrine) - Bacterial Expression mCitrine-N1... 548 562 49 6.5 2.5 hr Monomer pET mOrange LIC cloning vector (u-mOrange) - Bacterial Expression pCS2+...pmScarlet_C1 - Mammalian Expression pCS2+mScarlet-C Cloning Vector - Mammalian Expression mScarlet-I 569 593...587 610 16 4.5 15 min Monomer pcDNA3 mCherry LIC cloning vector (6B) - Mammalian Expression pLV-mCherry ...

... based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express...Cas-containing plasmids. gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9...

... based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express...Cas-containing plasmids. gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9...

... Protocols Subcloning Plasmid Cloning by Restriction Enzyme Digest (aka Subcloning) You may also like...Information about plasmid cloning by restriction enzyme digest (subcloning), including design and experimental...your recipient plasmid (usually in the Multiple Cloning Site (MCS)), but do not cut elsewhere on the plasmid...test digest later to verify that the insert was cloned in the correct orientation. If you cannot find ...sites to flank your insert : You can use PCR Based Cloning and add restriction sites to the ends of your oligos...of your recipient plasmid using Annealed-oligo Cloning . If you are lucky enough to have multiple options...instructions for your competent cells. For most standard cloning, you can transform 1-2μl of your ligation reaction...

...commonly used in molecular cloning techniques, such as PCR or restriction cloning . It is also used to quickly...typically involves ∼500 ng of DNA, while molecular cloning often requires 1 µg of DNA. The total reaction ...sufficient. For digests with >1 µg of DNA used for cloning, it is recommended that you digest for at least...finding an enzyme that cuts your vector's multiple cloning site (MCS), but does not cut your insert, you could...prevent re-circularization of the vector if you are cloning in an insert. CIP (calf alkaline phosphatase) or...

...truncated versions of SARS-CoV-2 S (spike protein) cloned into a CMV driven expression vector are also available...

... lentivirus is not always an easy process. The cloning capacity of lentiviral vectors (~8.5 kb of insert...

...my lab we do a lot of genetic construction and cloning experiments. Not all of our lab enzymes are made...

...Additional Resources on Addgene.org  Yeast-mediated cloning CRISPR plasmids: Yeast Yeast Synthetic Biology...

...biology. There are already two comprehensive plant cloning kits available in the repository, MoClo and GreenGate...

...BsaI digested vector. Cloning targeting chiRNAs Targeting chiRNAs are easily cloned by annealed oligos into...

...Plasmid Collection You may also like... Gateway Cloning Brain Initiative Collection NeuroMab Collection.... These vectors allow a fast and efficient sub-cloning into a wide range of Gateway-compatible pDEST vectors...

...Lentiviral vector for inducible expression for Gateway cloning; selection cassette in format: PGK-rtTA-2A-puro...shRNA; see article for detailed information about cloning and additional plasmids tetR-KRAB On Aebischer ...Elledge 35625 pAAV-Ptet-RFP-shR-rtTA AAV; shRNA cloning vector; for evaluation of shRNA efficacy using ...Sleeping Beauty transposon system; has luciferase in cloning site; see article for additional selection and ...

... Protocols Gibson Cloning Gibson Assembly Cloning You may also like... Cloning Methods...Assembly Cloning! Protocols...biologists now use this method extensively. Why Gibson Cloning? No need for specific restriction sites. Join almost...

... based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express...Cas-containing plasmids. gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9...

...event. Now it’s maybe one out of 100 or 50 event. Cloning of the second plasmid can be done in one day in...

... You can use DNA sequencing or other molecular cloning techniques to determine CRISPR/sgRNA efficiency...