We narrowed to 323 results for: cloning

Learn about xCas9, an engineering Cas9 variant with broad PAM flexibility and high on-target specificity.

SapTrap improves CRISPR genomic tagging in C. elegans by offering scientists an easy one tube assembly reaction to generate high-throughput targeting vectors.

Addgene scientists perform standard quality control for AAVs. But we are also going one step further with next-generation sequencing (NGS) of the viral genome.

Get tips on how to monitor your protein of interest inside cells - co-localization, complex formation, and more.

Guest blogger Pooran Dewari presents results of an NgAgo survey he gave to some of the first NgAgo users. Find out how NgAgo compares to Cas9 so far.

Learn how Cpf1 stacks up against Cas9 and NgAgo

Browse the Open Enzyme collection of plasmids at Addgene for generating enzymes for molecular biology lab work.

CRISPR plasmids for endogenous tagging of your gene of interest.

Learn about new tools in Addgene's collection, including new biosensors for neuroscience research, plasmids for a common lab enzyme, and more!

Learn about the advantages of using AAV as a delivery method for CRISPR screens.

Pooled libraries are a powerful tool, but they can difficult to prepare! Learn the tips and tricks for taking your pooled libraries from plasmids to screening.

Curious about the history of C-myc, one of the most common antibody tags? Learn all about it here!

Find plasmid tools for COVID-19 research expressing viral open reading frames, host factors, and tools for detecting SARS-CoV-2 and generating pseudoviruses.

Leaving the lab bench for a career outside of academic research can be a daunting task! In this blog post, we describe some ways to ease the transition from bench science to the office.

Find tips for transforming arabidopsis with your plasmid construct of interest.

Learn how and why Adeno-associated virus (AAV) has enabled breakthroughs in retinal gene therapy.

Fluorescent protein timers can be used to determine molecular age. Learn how to use these unique tools in this post.

Viral minigenomes provide a safe way to study dangerous viruses like Ebola. Read all about minigenomes inside.

Cpf1, a newly discovered Cas9 homolog, is smaller than Cas9, can target different sequences, and may more efficiently generate targeted mutations.

Explore empty plasmid backbones with different fluorescent tags to create fusion proteins with your gene of interest.