We narrowed to 599 results for: arl
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TypeBlog Post...is still important. It needs to express value, clearly communicate exactly what a site offers to their...communicate that sense of community, Blugene will regularly be paired with a variety of science-inspired friends...
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New Norepinephrine Indicators: nLightG and nLightR
TypeBlog Post...alpha-1a adrenergic receptor; nLightG contains circularly permuted green fluorescent protein (GFP), and...green fluorescent proteins. nLightR did perform similarly to GRABNE, and having both colors allows for increased... -
In Living Color: The Skinny on In Vivo Imaging Tools
TypeBlog Post... spectral region in which mammalian tissues are nearly transparent. As Verkhusha explained it to me, the...tissues 20-fold greater than any fluorescent protein earlier described. “Until our proteins, there were lots... -
CASTing Off for New Shores in Human Genome Editing
TypeBlog Post...popular bacterial genome engineering tools, with early reports of 40%–60% efficient integration (Klompe... al., 2019). Type I-F CASTs were found to be particularly useful. But they were barely effective at editing... -
Fostering communication in remote collaborations
TypeBlog Post...experiments. Therefore, it’s crucial that everyone meets early on to set expectations for the output that will ... which parts of the project, and, as mentioned earlier, how frequently the entire group will meet. You... -
Cellosaurus: A Cell Line Information Database
TypeBlog Post...new resources with the scientific community – particularly ones that help address ongoing problems, like...growing cell line information database describing nearly 145,000 cell lines. Cellosaurus Cellosaurus seeks... -
Hot Plasmids and Viral Preps - July 2021
TypeBlog Post...internal control and a SL-sensitive fluorophore (circularly permuted green fluorescent protein or cpGFP) ... New from the viral service By Jason Nasse We regularly add new viral aliquots from our plasmid collection... -
Tips for Improving Your Next Manuscript
TypeBlog Post...consisted of graduate students, post-grads, and early career scientists. Over the course of the class,...welcoming us. Then it was time to work! Set up similarly to the online class, we met as a group for sessions... -
Year of the Camelid: Antibody Style
TypeBlog Post... to manipulate cellular functions, like in this early example we highlighted back in 2015, where sdAbs... So where do all these sdAbs come from? In the early years of sdAbs development, the process started ... -
Running for Rare Disease, Running for FOP, Running for AJ
TypeBlog Post... in Cambridge, MA. When I joined Sanofi/Genzyme nearly three years ago I decided it was time to get in...analytical development. His areas of expertise include early stage drug discovery and pre-clinical development... -
Addgene at the ASM Microbe 2016 Conference
TypeBlog Post...Labeling Plasmids from the Barbier lab). Bright and early on Saturday morning, John Doench’s opening presentation... to the scientific community. However, from the nearly 4000 posters presented at the conference, it looks... -
Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene
TypeBlog Post...have been unwise of me to join Addgene. This is particularly true in my position as an Outreach Scientist...pretty hefty bumps during my graduate training. I particularly think that too many young scientists are led... -
Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio
TypeBlog Post...reagents so freely with the academic community has clearly made a huge impact on this field. Advances in ...answering biology customer service questions for nearly 7 years, and my colleagues and I have an internal... -
10 Ways to Share Your Science!
TypeBlog Post...some science on the one you already have. Read my earlier post about Scientists on Twitter here. 4) If ...commitment, start your own blog. Plan to write regularly and, when you are ready, tell your friends or ... -
Single-cell tracking of lineage and identity with CellTag
TypeBlog Post...time point, making it difficult to investigate how early expression patterns influence reprogramming outcomes...However, distinct transcriptional states were seen as early as day 6. All together these results suggest that... -
Safe Port in a Storm...How Addgene is Weathering the Pandemic
TypeBlog Post...expected to be in this here and now. Our closure was earlier than most companies. Since the start of this crisis... to make most of our payroll for the spring and early summer months. We are also extremely grateful to... -
What the HEK?
TypeBlog Post...two areas of research where HEK cells may be particularly useful due to their intrinsic properties. ...by mycoplasma and bacteria, but HEKs are also particularly vulnerable to viral infection. Yes, your cells... -
Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants
TypeBlog Post...Alpha variant), B1.1.298, and B.1.429 (Epsilon), nearly as well as they could for the wild-type. However...TM, Clayton KL, Nitido AD, Murali MR, Alter G, Charles RC, Dighe A, Branda JA, Lennerz JK, Lingwood D,... -
In Vivo Biotinylation of Bacterial Fusion Proteins
TypeBlog Post...plasmid in NEB T7 SHuffle bacteria (C3029J). We are clearly NEB “fanboys” as the bacteria significantly enhance...biochemist in the Vanderbilt Eye Institute and is particularly interested in cell signaling. Additional... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog Post...commercial plasmids were inconsistent and sometimes clearly wrong. In an effort to be rigorous, we dug into...Biology at the University of Chicago. He was an early depositor at Addgene, and conceived SnapGene because...