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  1. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ... to manipulate cellular functions, like in this early example we highlighted back in 2015, where sdAbs...  So where do all these sdAbs come from? In the early years of sdAbs development, the process started ...

  2. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    ...new resources with the scientific community – particularly ones that help address ongoing problems, like...growing cell line information database describing nearly 145,000 cell lines. Cellosaurus Cellosaurus seeks...

  3. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    ...Labeling Plasmids from the Barbier lab). Bright and early on Saturday morning, John Doench’s opening presentation... to the scientific community. However, from the nearly 4000 posters presented at the conference, it looks...

  6. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...some science on the one you already have. Read my earlier post about Scientists on Twitter here. 4)   If ...commitment, start your own blog. Plan to write regularly and, when you are ready, tell your friends or ...

  7. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...We have also packaged hChR2(H134R) plasmids from Karl Deisseroth’s lab, which includes pAAV-hSyn Con/Fon...WPRE-SV40 124650 AAV9 pAAV-CamKIIa-C1V1(t/t)-mScarlet-KV2.1 55645 AAV5 pAAV-hSyn Con/Fon hChR2(H134R...

  8. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    ...better, no grant writing Job changes can happen regularly (every 4-6 years is common), but this can also...diverse options and how to take advantage of them early is one way to smooth your path to a fulfilling career...

  11. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...plasmid in NEB T7 SHuffle bacteria (C3029J). We are clearly NEB “fanboys” as the bacteria significantly enhance...biochemist in the Vanderbilt Eye Institute and is particularly interested in cell signaling.     Additional...

  13. What the HEK?

    Type
    Blog Post
    ...two areas of research where HEK cells may be particularly useful due to their intrinsic properties. ...by mycoplasma and bacteria, but HEKs are also particularly vulnerable to viral infection. Yes, your cells...

  14. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...time point, making it difficult to investigate how early expression patterns influence reprogramming outcomes...However, distinct transcriptional states were seen as early as day 6. All together these results suggest that...

  16. Celebrate National Book Month with Addgene

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    Blog Post
    ...insects, mammals, and plants, among others. I particularly enjoyed the emphasis that microbes are neither...last 8 years. Since then, we’ve read books across nearly every genre you can imagine: gothic, mystery, sci-fi...

  19. The MTA at Addgene

    Type
    Blog Post
    ...MTAs by the Provider and Recipient (in Addgene parlance, the depositor and requestor) organizations. Over...depends on things being organized, tracked, and clearly communicated. And that’s where MTAs, and similar...

  20. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...because they demonstrate high promoter activity (Earley et al. 2020), but non-AAV2 ITRs can be used to ...Addgene's AVV guide AAV Viral Preps References  Earley, L. F., Conatser, L. M., Lue, V. M., Dobbins, A...
Showing: 201 - 220 of 560 results