...Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI...Promoter Selectable Marker PI Publication Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker ...Publication Empty Prime Editing gRNA Vectors A selection of empty gRNA vectors suitable for prime editing...pegRNA, epegRNA, nicking sgRNA), cloning enzyme, selectable marker, and whether the plasmid contains Cas9...Guide RNA Type Cloning Enzyme Co-expressed Cas9 Selection PI 132777 pU6-pegRNA-GG-acceptor Mammalian hU6...

... you can find or select for only the cells that received the plasmid. Selectable Marker...curated by species, epitope tags/fusion protein, selectable marker, viral expression, and more. Plasmid...Species-specific expression Epitope tag or fusion protein Selectable markers Viral expression and packaging Reporters... our list of common tags . Back to Top Selectable Markers Regardless of your delivery method, it's...retroviral gene expression, multiple selectable markers pMKO.1 puro - Mammalian ... retroviral gene expression with GFP selectable marker LZRS-Rfa or pMXs-gw - Mammalian...

...expression of shRNA; neomycin selection; plasmid 21915 has puromycin selection TetR On Wiederschain 41393...miR-shRNA; selection cassette in format: rtTA3+IRES+Neo; see article for additional selection options third-generation...for inducible expression for Gateway cloning; selection cassette in format: PGK-rtTA-2A-puro; see article...luciferase in cloning site; see article for additional selection and FP option rtTA On Kowarz 16623 pBI-GFP Expression..., and Puromycin was replaced with Blasticidin selection. Please NOTE, this vector contains an insert (...

...expression of shRNA; puromycin selection. See plasmid 21916 for Neo selection. Wiederschain 11578 pSico 3rd...with EGFP marker. See plasmid 14749 for Thy1.1 selection. Benoist and Mathis 21915 Tet-pLKO-puro 3rd inducible...shRNAs or miRNAs, and use a wide variety of drug selection markers. See article for more variations. Campeau...-puro 3rd CMV driven expression of cDNA. Puro selection. Shih 25737 pSLIK-Hygro 3rd Tet-based inducible...Tet-pLKO-neo 3rd inducible expression of shRNA; neomycin selection. See plasmid 21915 for Puro resistance. Wiederschain...

...Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI... Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI ...Yeast ID Plasmid Description Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions...

...Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI... Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI ...Publication Zebrafish ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions...

... and SpCas9, but does not contain markers for selection 1 . Other constructs may be utilized, such as ...ID 48139), which include GFP and puromycin as selectable markers, respectively, or constructs in which...Screening CRISPR/Cas9 Clones for Deletions and Clone Selection For suspension cells, transfer all clones to a...conditions optimized on the bulk cells (see step 6 ). Select the clones identified with the desired deletion...onto LB agar plates with the relevant antibiotic. Select multiple colonies, mini-prep each one, and subject...initial screen ensures that the correct clone was selected and reproducibility of results. To evaluate the...

...Addgene’s data processing needs, our criteria for selecting a new workflow management system, and how Nextflow...

...name was submitted by more than one person, we selected the finalist based on the first time of submission...

... the third day of CRISPR, Addgene gave to me: Selective Endogenous eNcapsidation for cellular Delivery...

...removing Stop sequences; Puro selection. See plasmid 35688 for Hygro selection. Mammalian Jacks 11925 pBS302...We also offer ready-to-use viral preparations of select Cre expression plasmids. Addgene's viral service...pBS-OMP-CreFNF OMP targeting vector with Cre FNF (neo-selectable marker flanked by FRT sites) Mammalian Mombaerts...IRES-CreFNF M71 targeting vector with IRES CreFNF (neo-selectable marker flanked by FRT sites) Mammalian Mombaerts...evaluating infection/transfection efficiency, selecting desired clones, and making transgenic mice. ID...Hughes 16422 betaRBPIG Double fluorescent, double selectable cre/loxP reporter Mammalian Hescheler 12463 p231...

...ll enter your name and email address. If you’re selected in the raffle, you will receive an email from ...

...                     A post shared by Xi Chen (@seleena78)   Honorable Mention: Culture Warrior The Moore...

...Guide are probably the papers and textbooks. I’ve selected “seminal” studies for various categories, including...

...Simon Moe, from Promega, who wrote a guide to selecting fluorescent dyes and ligands. Illuminating Choices...

...more about the biophysics of protein folding and selective pressures during protein evolution. The Baker ...

... on “How to Order” details every stage of the selection and checkout process on Addgene’s website: We...

... the best students in his home country. He was selected as one of 600 scholars among 100,000 eligible ...

...pooled screening approaches; (4) encodes a Thy1.1 selectable marker to facilitate the isolation of modified...

...on the SARS-CoV-2. Nine of the overall plasmid selection will be open to industry scientists. This collection...