... for clear communication, fair credit through authorship, and acceptance of a diversity of work styles...stress the fact that “miscommunication, a lack of leadership, and inappropriate tools or writing approaches...that was probably lost during these weird times of shift working and social distancing. The take home message...

...returned the MTA, Addgene can move forward with shipping the material to the requestor.  Why the MTA?  ...certainly frustrating to find out your plasmid hasn’t shipped because a form needs to be signed. But the truth... And that’s where MTAs, and similar agreements, shine.  These types of agreements allow for centralized...

...however, it requires high doses. Requests have shifted toward the more efficient AAV-PHP.eB, developed...Evolutionary tree depicting the phylogenetic relationship of common research species. Among these species..., Kim, H.M., Rajendran, P.S., Tompkins, J.D., Shivkumar, K., Deverman, B.E., Gradinaru V. Systemic AAV...0799-7 Lawler, A. J., Ramamurthy, E., Brown, A. R., Shin, N., Kim, Y., Toong, N., Kaplow, I. M., Wirthlin....017 Matsuzaki, Y., Konno, A., Mochizuki, R., Shinohara, Y., Nitta, K., Okada, Y., & Hirai, H. (2018)....

...the US and Canada convene at ComSciCon's annual flagship workshop.  Our 2018 workshop took place in Boston...students, and we pay for travel expenses to our flagship workshop. In our view, the best possible outcome...is a co-founder of ComSciCon and Chair of its Leadership Team.  Nathan did his PhD in astronomy and works...

...launched the BioBuilder Educational Foundation in partnership with high school teachers to boost interest and...students include The BioBuilder High School Apprenticeship, preparing local (Massachusetts), economically...economically disadvantaged students for paid summer internships. A summer teacher professional development program...

...30082838. PubMed Central PMCID: PMC6078992. Nishimasu, Hiroshi, et al. "Engineered CRISPR-Cas9 nuclease ...which required only the di-nucleotide PAM NG (Nishimasu et al., 2018). The David Liu Lab similarly described...

...Cancer and the Immune System: Deciphering the Relationship” by our guest blogger Subhadra Jayaraman, a ... 113-117. PubMed PMID: 28225754. 6. Marumoto, Tomotoshi, et al. "Development of a novel mouse glioma model...Cancer and the Immune System: Deciphering the Relationship Resources on Addgene.org Browse Cancer Plasmids...

...?) As a result, I’d always end up stressed and rushing, no matter how hard I tried to build in extra time...especially on days when I was tired, stressed, or rushing. I would spend the time quietly running through... consider it this way: When you’re stressed or rushing, you’re much more likely to make a mistake. When...

...and target DNA. Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, Ishitani R, Zhang F, Nureki...OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F. Nature. 2015 Jan 29;517(...

...mCyRFP1 include a redshifted-emission spectrum compared to that of CyOFP1, a large stokes shift, long fluorescence...s properties. pBAD-LSSmCherry1 is a long Stokes shift variant, which could be compatible with two-photon... Ti-Sapphire lasers. pBAD-RDSmCherry1 is a red-shifted variant which, with further development, could ...

.... PubMed Central PMCID: PMC4517063. 3. Mizushima, N., Yoshimori, T., & Levine, B. (2010). Methods in Mammalian...Pho8 phosphatase activity or the molecular weight shift of Pho8 from its uncleaved to cleaved form is the...

...Therapeutics Graduate Program has a compulsory internship to be taken in an industry, regulatory, or clinical...many notable contributions in this area include publishing the book ‘How Economics Shapes Science’ and contributing...employ them, the diversity of trainee backgrounds, publishing from a young scientist’s perspective, and the...

...plate with every plasmid or as individual plasmids shipped as agar stabs. Iverson, et al. ACS Synth Bio. ...likelihood of creating a functional knock-out via frameshift and minimize off-target effects. The library ...Destination series, pCAG-Golden-Gate-Esp3I-Destination Takashi Yamamoto pcDNA-TAL-NC2, pCAGGS-TAL-NC2 Charles... et al. Cell Rep. 2016. PubMed PMID: 27052166 Nishimura K, et al. Nat. Methods 2009. PubMed PMID: 19915560... prototrophy, can also aid in designing self-establishing metabolically cooperating (SeMeCo) communities...

...FRET vectors. Murakoshi H, et al. Sci Rep. 2015. Pubmed PMID: 26469148. Murakoshi H, et al. Sci Rep...segment Article contributed by Christina Mork The Murakoshi lab has developed two new FLIM-FRET acceptors,...

...all of your plasmid data, we’ll send you prepaid shipping materials (i.e., a deposit kit) with instructions...we need to know your Addgene account username. Shipping address and phone number. Plasmid sequences or...deposit kit with instructions on how to prepare and ship the samples to Addgene and contact your technology...

...01039-7 Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I., Dohmae, N., Ishitani, R., ...disrupting its canonical structure and Cas9 binding (Nishimasu et al., 2014). Figure 3: pegRNAs whose...

...Similarly, if you want to knock out a gene, a frameshift mutation introduced anywhere within the early...with NG PAM compatibility, as the name implies (Nishimasu et. al, 2018). xCas9 was generated by the Liu ...10.1126/science.aba8853 (Plasmids available here)   Nishimasu, H., et al.,Engineered CRISPR-Cas9 nuclease with...

...– $30,000 for research and another $30,000 in mentorship and business training. The money is a drop in...thinking about making the switch from academia to entrepreneurship, share your thoughts with us in the comments...guest bloggers! Nikolai Braun grew up outside of Washington D.C. and has always enjoyed exploring the outdoors...

... situation where one wants to understand the relationship between the age of a cell, protein, or cellular...: 1585-1588. PubMed PMID: 11090358. 2. Tsuboi, Takashi, et al. "Age-dependent preferential dense-core ... 118-126. PubMed PMID: 19136976. 5. Miyatsuka, Takeshi, Zhongmei Li, and Michael S. German. "Chronology...

...environment sooner rather than later can be helpful. Publishing: As a postdoc at AstraZeneca, you are expected...vary depending on which company you choose. If publishing is important to you, make sure that your project...an industry setting. Scientific community and mentorship: As an industry postdoc, you might find yourself...