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  1. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...mStrawberry-N1 - Mammalian Expression FusionRed 580 608 18 Monomer FusionRed-pBAD - Bacterial Expression mRFP1...with different fluorescent tags for you to create fusion proteins with your gene of interest. Special... Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs Blog: When is a Monomer not a Monomer...with different fluorescent tags for you to create fusion proteins with your gene of interest. These are ...Snapp . Empty Backbones for Fluorescent Protein Fusions (Organized by Color) For each table: Excitation...Expression (this is a bicistronic vector rather than a fusion protein vector) ZsGreen1-N1 - Mammalian Expression...to Top Empty Backbones for Fluorescent Protein Fusions (Organized by Expression Species) Organism/Expression...
  2. Editor's Choice, August 2016

    Type
    Blog Post
    ...catalytically inactive CRISPR endonuclease (dCas9) fused to a deaminase to make the transitions at target...
  3. Lentiviral Guide

    Type
    Guide
    ...: Tet- or Cre-based regulation and fluorescent fusions or reporters. Browse lentivirus plasmids available...system through the addition of a chimeric 5' LTR fused to a heterologous promoter on the transfer plasmid...Wild type Hybrid: 5'LTR is partially deleted and fused to a heterologous enhancer/promoter such as CMV ...
  4. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ... it have a lot of parts! If you’re still a bit confused by CRISPR acronyms, this post is for you. We’ll...
  5. Optogenetics AAV Preps

    Type
    Collection
    ...105669 pAAV-CKIIa-stGtACR2-FusionRed CaMKII GtACR2 (soma-targeted) FusionRed Constitutive 1, rg* Yizhar...105677 pAAV_hSyn1-SIO-stGtACR2-FusionRed Syn GtACR2 (soma-targeted) FusionRed Cre dependent 1, 5, rg* Yizhar...105678 pAAV_hSyn1-SIO-stGtACR1-FusionRed Syn GtACR1 (soma-targeted) FusionRed Cre dependent 5 Yizhar 109048...
  6. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...stranded breaks. Cytosine base editors are created by fusing Cas9 nickase or catalytically inactive “dead” Cas9...cytosine base editors, the evolved TadA domain is fused to a Cas9 protein to create the adenine base editor...have been made by optimizing expression of the fusions, modifying the linker region between Cas variant...deaminase to adjust the editing window, or adding fusions that increase product purity such as the DNA glycosylase...CRISPR-X from the Bassik lab , in which Cas9 is fused to activation-induced cytidine deaminase (AID). ...
  7. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ...Scanziani New viral prep pAAV_hSyn1-SIO-stGtACR2-FusionRed AAV5 Optogenetics Ofer Yizhar New serotype pAAV-CaMKIIa-ChRmine-oScarlet-KV...
Showing: 201 - 220 of 363 results