... Labs containing wild-type and mutant alleles described in the initial Target Accelerator publications...

...comprehensive high-quality quality control process includes titering, purity assessment, sterility testing...

... are highly absorbed by hemoglobin and cause undesirable background signals. Near-infrared fluorescent...

...together based on type or application. Browse our guides to find the plasmids and science information you...

...Browse depositor protocols, find software for gRNA design and deep sequencing analysis, discover links to...

...Cre-dependent Cre-dependent, bicistronic expression of designer pro-taCasp3 and TEVp for studying cell ablation...

... that we will not meet the turnaround time we described here or by email, Addgene will contact you to ...

...Vectors for Monosynaptic Neuronal Tracing ID Name Description Serotype PI 52473 pAAV-synP-FLEX-splitTVA-EGFP-B19G...

...Plasmids by Gene Name Alternative Gene Names Gene Description AR plasmids AR, RP11-383C12.1, AIS, DHTR, HUMARA...

...protocols, titering methods, and quality control are described below. Information for a specific antibody can...

...all-in-one pricing for viral vector preps, which includes MTA facilitation, DNA amplification, and high-...

...fluorescent protein. Plasmid Color Expression Description pPROEX Aqua Cyan Bacterial Expresses Aquamarine...

...name for ordering information. ID Name Promoter Description Category PI 157970 pTR-UF11 chimeric CMV/Chicken...

...protocols, titering methods, and quality control are described below. AAV Production AAV distributed by Addgene...

...using in mouse transgenics. Designing an Easi-CRISPR knock-in allele To design a knock-in allele at a given... PAM or guide mutation. Designing an Easi-CRISPR conditional allele The design for a floxed conditional...successfully used guides targeting the same or complementary strands, as well as guides facing towards or...How to design a conditional allele with Easi-CRISPR. As in the knock-in example, you’ll design your ...with your desired insertion feature (e.g., a fluorescent protein), you can amplify the desired region using... Figure 5: How to design Easi-CRISPR repair templates using Addgene plasmids. Design PCR primers to add...arduous. First, you’d construct a plasmid with your desired changes, long homology arms and a selection marker...

...relaxed gRNA design parameters. To demonstrate the effectiveness of their library design, Moffat laboratory...-strand DNA (ssDNA) oligonucleotides (oligos), and thereby rapidly create desired allele combinations ... pCAG-T7-TALEN(Sangamo)-Destination series, pCAG-Golden-Gate-Esp3I-Destination Takashi Yamamoto pcDNA-TAL-NC2...vector, and besides being used for restoring strain prototrophy, can also aid in designing self-establishing...assay. In their recent publication, Kroeze et al. describe their enhanced TANGO arrestin recruitment assay...optimized, expression validated GPCR sequences to any desired backbone.  Kroeze, et al. Nat Struct Mol Biol....plasmids with the Cross-disciplinary Integration of Design Automation Research lab Moduclar Cloning (CIDAR...

...clone in their desired DNA sequences. Gateway technology relies on the two reactions described below: The ...pLenti CMV Puro DEST (w118-1) or the doxycycline-inducible pLIX_403. The chosen attR destination vector will...between the entry clone and destination vector creates two products: the desires expression clone and a byproduct...produce the desired expression clone. This is possible thanks to the Gateway vectors’ design. They have...vector systems. With the appropriate entry and destination vectors, one can use Gateway to clone a gene ...generated entry clone and the attR sites of the destination vector. This reaction is catalyzed by the LR ...containing the ccdB gene is excised from the destination vector.  Once the BP and/or LR reactions are ...

....  These primers are typically ~70 nucleotides long (20 nucleotides that anneal to the DNA sequence of..., the substrate should be ~70-100 nucleotides long with the desired alteration(s) located in the center...genomes. 2) Designing ssDNA oligos that avoid MMR activation:  There are two ways to design oligos that...tube. The donor DNA substrate only requires ~50 nucleotides of homology to the target site for recombination... be explained in greater detail. Substrate DNA design and generation Expression of lambda red recombination...antibiotic resistance cassette.   Substrate DNA Design and Generation Whether you use a linear dsDNA or...insertions or deletions greater than approximately 20 nucleotides, while ssDNA substrate is best for point mutations...

...list of Fragmid destination vectors currently available. Table 1: Fragmid destination vectors Organism...website, which breaks the design process down into four easy steps, provides the real value for the user...potential of them not working, or spend a few weeks designing and cloning the vectors you actually want. But... Fragmid is a modular vector assembly process designed for a quick, plug-and-play approach to cloning...et al., 2024). It reduces the amount of time to design and create a new vector to four days for an experienced...high school interns, were able to use Fragmid to design and clone their vectors in five days. Did you say...s modular assembly structure takes a lot of the design work out of the plasmid creation process. Each ...

...preprint title or a general description of the deposit. Select your desired distribution status: Hold...choose from one of the following descriptions for your plasmid: Encodes insert(s) A plasmid with one or...should describe how your plasmid is intended to be used. For instance, if your plasmid is designed to allow...Unpublished or pre-publication plasmids" and set a desired distribution status. You can submit "Published ...plasmids with the original publication where you described them. Search for your article as you would search...selected your publication or entered your deposit description, the next page will allow you to add plasmids... article. Note that if you entered a deposit description, this will become your article title.   ...