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We narrowed to 968 results for: Cre

Showing: 201 - 220 of 968 results
  1. CRISPR Plasmids - dCas9-FokI

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    Collection
    ...CRISPR plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs)....Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...
  2. KLF Research Plasmids

    Type
    Collection
    ...plasmids and learn more about the scientists who have created and shared these plasmids....of this shared resource on Addgene. If you have created plasmids containing KLF, we encourage you to contribute...
  3. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...fused to a transcriptional activator peptide can increase transcription of a specific gene. Design your ...locus. Figure 1: Overview of CRISPR activation. Created with BioRender.com. Browse, sort, or search the...
  4. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ... modifiers include: P300 — activation through increased H3K27 acetylation TET1 — activation through cytosine...Overview of epigenetic modification using CRISPR. Created with BioRender.com. Browse, sort, or search the...
  5. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ...breaks in DNA instead of the double-strand breaks created by wild-type Cas enzymes. To use a nickase mutant...your DNA in close proximity. These double nicks create a double-strand break (DSB) that is repaired using...
  6. Structural Genomics Consortium Plasmids

    Type
    Collection
    ...AmpR 26095 pFHMSP-LIC-N N/A Expression vector for secreted proteins with N-terminal hexahistidine tag and...AmpR 26100 pFHMSP-LIC-C N/A Expression vector for secreted proteins with C-terminal hexahistidine tag and...
  7. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...CRISPR plasmids that create double strand breaks in DNA.... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific...
  8. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific...
  9. CRISPR Plasmids - Parasites

    Type
    Collection
    ...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific...
  10. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...to deletions generated by Cas9, large deletions created using Cas3 have fewer off-target alterations and... Figure 1: Overview of Cascade-Cas3 mechanism. Created with BioRender.com. Browse, sort, or search the...
  11. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...paper . How to Cite this Kit These plasmids were created by your colleagues. Please acknowledge the Principal...Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods...
  12. CRISPR Plasmids and Resources

    Type
    Collection
    ...Other Applications Screen CRISPR libraries are a powerful tool for genome-wide screening. Purify dCas9 fused...
  13. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ...each (these can be built into PCR primers when creating a new part, with an additional 5' clamping sequence...Features: Modified RFC 10 w/ start and stop codons; Creates in-frame fusions w/ linker Thr-Gly; Use N-parts...
  14. Viral Vector Guides and Plasmids

    Type
    Collection
    ...generate viral vectors for research and gene therapy. Created with BioRender.com. Wild-type viruses have one ...vehicles to deliver genetic cargo into cells. To create safe and effective viral vectors, wild-type viral...
  15. Microbiology Resources

    Type
    Collection
    ...used to reconstitute self-replicating microbes or recreate the diseases they cause. The standard format for...system - Wittbrodt Lab MultiSite Gateway Kit : Creating three-part fusion proteins - Planas and Santalucia...
  16. Lentiviral Prep Service

    Type
    Collection
    ...Pooled CRISPR Libraries for Screening Use pooled CRISPR libraries to screen for genes involved in specific...
Showing: 201 - 220 of 968 results