We narrowed to 968 results for: Cre
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TypeCollection...CRISPR plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs)....Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...
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KLF Research Plasmids
TypeCollection...plasmids and learn more about the scientists who have created and shared these plasmids....of this shared resource on Addgene. If you have created plasmids containing KLF, we encourage you to contribute... -
CRISPR Plasmids - Activate Gene Expression
TypeCollection...fused to a transcriptional activator peptide can increase transcription of a specific gene. Design your ...locus. Figure 1: Overview of CRISPR activation. Created with BioRender.com. Browse, sort, or search the... -
CRISPR Plasmids - Epigenetics
TypeCollection... modifiers include: P300 ā activation through increased H3K27 acetylation TET1 ā activation through cytosine...Overview of epigenetic modification using CRISPR. Created with BioRender.com. Browse, sort, or search the... -
CRISPR Plasmids - Single-Strand Break (Nick)
TypeCollection...breaks in DNA instead of the double-strand breaks created by wild-type Cas enzymes. To use a nickase mutant...your DNA in close proximity. These double nicks create a double-strand break (DSB) that is repaired using... -
Structural Genomics Consortium Plasmids
TypeCollection...AmpR 26095 pFHMSP-LIC-N N/A Expression vector for secreted proteins with N-terminal hexahistidine tag and...AmpR 26100 pFHMSP-LIC-C N/A Expression vector for secreted proteins with C-terminal hexahistidine tag and... -
CRISPR Plasmids - Double-Strand Break (Cut)
TypeCollection...CRISPR plasmids that create double strand breaks in DNA.... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific... -
CRISPR Plasmids - Zebrafish
TypeCollection...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific... -
CRISPR Plasmids - Parasites
TypeCollection...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific... -
CRISPR Plasmids - Cascade-Cas3
TypeCollection...to deletions generated by Cas9, large deletions created using Cas3 have fewer off-target alterations and... Figure 1: Overview of Cascade-Cas3 mechanism. Created with BioRender.com. Browse, sort, or search the... -
Zinc Finger Consortium: OPEN Reagents
TypeCollection...paper . How to Cite this Kit These plasmids were created by your colleagues. Please acknowledge the Principal...Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods... -
CRISPR Plasmids and Resources
TypeCollection...Other Applications Screen CRISPR libraries are a powerful tool for genome-wide screening. Purify dCas9 fused... -
Synthetic Biology - Assembly Standards Guide
TypeCollection...each (these can be built into PCR primers when creating a new part, with an additional 5' clamping sequence...Features: Modified RFC 10 w/ start and stop codons; Creates in-frame fusions w/ linker Thr-Gly; Use N-parts... -
Protocol - Over-Agar Antibiotic Plating
TypeProtocol... to do the spreading; the tip is gently bent to create an āLā shape, and then used like a cell spreader... -
Viral Vector Guides and Plasmids
TypeCollection...generate viral vectors for research and gene therapy. Created with BioRender.com. Wild-type viruses have one ...vehicles to deliver genetic cargo into cells. To create safe and effective viral vectors, wild-type viral... -
Protocol - How to Purify DNA from an Agarose Gel
TypeProtocol...better resolution of bands? A couple simple ways to increase the resolution (crispness) of your DNA bands include... -
Microbiology Resources
TypeCollection...used to reconstitute self-replicating microbes or recreate the diseases they cause. The standard format for...system - Wittbrodt Lab MultiSite Gateway Kit : Creating three-part fusion proteins - Planas and Santalucia... -
Allen Institute for Cell Science Plasmid Collection
TypeCollection... used in the creation of the hiPS cell lines The following plasmids can be used to create cell lines with... -
Lentiviral Prep Service
TypeCollection...Pooled CRISPR Libraries for Screening Use pooled CRISPR libraries to screen for genes involved in specific... -
Protocol - How to Perform a Diagnostic Digest
TypeProtocol...with both and seeing both patterns you can be incredibly confident that you have the correct plasmid. ...