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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 957 results for: Cre

Showing: 201 - 220 of 957 results

  1. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...an antibody. This protocol will describe how to create a standard curve using known concentrations of ...be empirically determined. The dilution series created in section 1, step 2 is a suggested starting point...

  2. AAV Production in HEK293 Cells

    Type
    Protocol
    ...harvesting. The addition of sorbitol has been shown to increase viral titers by up to 1.8-fold ( Rego et al., ...sodium bicarbonate, sorbitol has been found to increase viral titers by up to 1.8-fold. See the recipe...

  3. Michael J Fox Foundation (MJFF) Plasmid Collection

    Type
    Collection
    ...expressing genes relevant to Parkinson's disease created by the Michael J. Fox Foundation...topic. Michael J. Fox Foundation Plasmids MJFF has created a number of plasmids for expression of variants...

  4. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...CRISPR plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs)....Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...

  5. KLF Research Plasmids

    Type
    Collection
    ...plasmids and learn more about the scientists who have created and shared these plasmids....of this shared resource on Addgene. If you have created plasmids containing KLF, we encourage you to contribute...

  6. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...activity, experimental considerations, and more, created and maintained by the Doudna Lab . Browse, sort...

  7. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...activity, experimental considerations, and more, created and maintained by the Doudna Lab . Browse, sort...

  8. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...activity, experimental considerations, and more, created and maintained by the Doudna Lab . Browse, sort...

  9. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific...

  10. CRISPR Plasmids - Parasites

    Type
    Collection
    ...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific...

  11. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...Figure 1: Overview of the basic CAST mechanism. Created with BioRender.com. Browse, sort, or search the...

  12. Structural Genomics Consortium Plasmids

    Type
    Collection
    ...AmpR 26095 pFHMSP-LIC-N N/A Expression vector for secreted proteins with N-terminal hexahistidine tag and...AmpR 26100 pFHMSP-LIC-C N/A Expression vector for secreted proteins with C-terminal hexahistidine tag and...

  13. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...paper . How to Cite this Kit These plasmids were created by your colleagues. Please acknowledge the Principal...Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods...

  14. CRISPR Plasmids and Resources

    Type
    Collection
    ...Other Applications Screen CRISPR libraries are a powerful tool for genome-wide screening. Purify dCas9 fused...

  15. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ...each (these can be built into PCR primers when creating a new part, with an additional 5' clamping sequence...Features: Modified RFC 10 w/ start and stop codons; Creates in-frame fusions w/ linker Thr-Gly; Use N-parts...

  16. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...another round. Figure 1: Overview of prime editing. Created with BioRender.com. The original prime editing ...

  17. Microbiology Resources

    Type
    Collection
    ...used to reconstitute self-replicating microbes or recreate the diseases they cause. The standard format for...system - Wittbrodt Lab MultiSite Gateway Kit : Creating three-part fusion proteins - Planas and Santalucia...

  19. Lentiviral Prep Service

    Type
    Collection
    ...Pooled CRISPR Libraries for Screening Use pooled CRISPR libraries to screen for genes involved in specific...

  20. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...Addgene has a collection of plasmids suitable for creating individual fluorescently tagged proteins to study... for FRET The following plasmids can be used to create a fluorescent fusion protein with your gene of ...
Showing: 201 - 220 of 957 results