... generally two to three fold higher than those achieved using the standard qPCR method.  Titering by the...preparations is assayed by comparing the relative stoichiometric ratios of the viral capsid proteins VP1, VP2...transfer plasmid Addgene uses a rigorous barcode matching system that follows the sample from the DNA tube...    Figure 4: Schematic of the barcode matching system and PCR-based confirmation of transfer ...

...plasmids rely on their host cell's replication machinery in order to replicate—but not always to the same..., as they only require the host's replication machinery for elongation and termination. These types of... folding and blocks RepZ translation (Asano & Mizobuchi, 1998). This system keeps RepZ levels low even...doi.org/10.5772/19313 References Asano, K., & Mizobuchi, K. (1998). Copy number control of IncIalpha plasmid...

...general, or anxiety associated with psychiatric diseases such as schizophrenia or bipolar disorder. In both ...found that downregulating expression of miR124a achieved a similar improvement in the forced swim test....the BDNF hypothesis of depression?." Molecular psychiatry 12.12 (2007): 1079. PubMed PMID: 17700574. Hare...

...   Many thanks to our guest blogger, Joachim Goedhart! Joachim Goedhart is an assistant professor at ...This post was contributed by guest blogger Joachim Goedart, an assistant professor at the Section of ...fluorescent probes. You can follow him on twitter: @joachimgoedhart.   References 1. Zacharias, David A., et al...

... are part of engineered chimeric antigen receptors (CARs). CARs are a chimera of scFvs and T cell receptors...anti-digoxin single-chain Fv analogue produced in Escherichia coli. Proceedings of the National Academy of ...A, Sivaccumar JP, Ruvo M (2020) Evolution of Escherichia coli Expression System in Producing Antibody ...

...1: Indirect detection of target markers can be achieved through two-protein labeling by utilizing(a) biotin-streptavidin...e.g., for cellular activation. In addition, FACS achieves greater purity of your sorted cell sample than...proliferation between cell populations can be visualized, achieved through, for example, a previously induced gene... your marker of interest. Indirect labeling is achieved through protein-tag or protein-protein interactions...

...pseudopregnant female mice. If you obtained the desired chimeric mice, you would breed them many times, hoping ...line with a recombinase. If it turned out your chimeric mice did not contain edited germ cells, your experiment...inject the ES cells into a mouse embryo to create chimeric mice. This laborious process takes ~8-10 months...When delivering the ssDNA template and CRISPR machinery, Quadros et al. recommend ribonucleoprotein (RNP...

...engineered from the cmt and cym bacterial operons. A chimeric transactivator, cTA, was created by fusing CymR...the system, researchers have used split GAL4 architecture or combined the system with Flp/FRT, as reviewed...) to GAL4 or VP16 activation domain results in chimeric GAL80-repressible and GAL80-insensitive activators...lethality in flies. Riabinina et al. also developed chimeric transactivators GALQF and LexAQF, which activate...

...have created many engineered serotypes by mixing, matching, and modifying capsid and genomic structures. ...., Brown, K. E., Walters, R. W., Zabner, J., & Chiorini, J. A. (2001). Adeno-Associated Virus Serotype...Kuleshova, E., Ojala, D., Huang, C.-C., Gerfen, C. R., Schiller, J., Dudman, J. T., Hantman, A. W., Looger, L....M., Keshavjee, S., Brown, K. E., Welsh, M. J., Chiorini, J. A., & Zabner, J. (2001). Binding of adeno-...

...In an era where millions of people hold a PhD, achieving our career goals can be a real challenge. On top... hinder them from advancing their careers and achieving success.” Pedro Gonzalez Menendez (Mentee IMP ...compared to their pre-program self-assesment. Achievements directly attributable to participation in the...interested in breast cancer and metastasis as well as teaching and mentoring activities. Follow her on twitter...

...optimal/minimal injection titer and volume needed to achieve adequate expression in your cells of interest.  ...waiting periods and/or higher injection titers to achieve adequate expression. Conducting a time course experiment...to minimize off-target expression while still achieving adequate expression in your cells of interest....different brain region that the same result will be achieved. The fact is that there are many known and unknown...

...easy for you to share plasmids. To achieve this goal, we will archive any plasmids you've deposited with...submission of QUEEN-generated GenBank files (Mori and Yachie, 2021) but can accept sequence files in any format...submission of QUEEN-generated GenBank files (Mori and Yachie, 2021). Figure 5: Add sequence, maps, and files...

...2. Fluorescence Activated Cell Sorter (FACs) A machine that sorts cells according to whether or not they... deflect them into appropriate containers. The machine is useful for distinguishing cell populations that...21596592. PubMed Central PMCID: PMC3164316. 5. Chieppa, Marcello, et al. "Dynamic imaging of dendritic...165. PubMed PMID: 18193049.  9. Filipe-Santos, Orchidée, et al. "A dynamic map of antigen recognition ...

...2017 contest winners:   Chicagoland's Community Biology Lab (@ChiTownBio) and one of our favorite twitter...holiday cheer, and we loved it! Check out their matching "MACK ATTACK!" holiday sweaters. Alex Dainis ...

...business efforts at Addgene as the Chief Financial Officer and Chief Operating Officer. Prior to Addgene... Director of Technology for 7 years, and is now Chief Software Engineer at Ginkgo Bioworks. He also holds...

...various cloning methods and tools to construct chimeric cellulases that enable E. coli and Citrobacter...plans for these plasmids have a heavy focus on teaching. He hopes to teach his molecular biology students...Diseases Read About Some of Our Outreach Efforts in China Additional Resources on Addgene.org Learn About...

... members Tony Cova and Chiara DeNatale and programming with our Chief Architect Pam McA' Nulty (check ...

...would be a big deal if it worked out.”- Melina Fan, Chief Scientific Officer “Choose your PhD lab carefully...opportunity.” - Sophia Cheng, Senior Software Engineer “Searching for a new job can be an imposing endeavor.  Break...the place that you are applying.” - Melina Fan, Chief Scientific Officer If there’s a career topic that...

...therapy, Sacher and Zheng also saw the need to archive phage reagents. If a phage researcher retires, ...likely that the phages are lost if they are not archived. “It’s very sad because it’s years of work,” Sacher... it can also fill this role by cataloging and archiving information on individual phages. Integrating ...

...method that uses iterative staining and chemical bleaching to allow for high-resolution imaging without degrading... Yaniv, Z., Yao, L., Marr, J., Beuschel, R. T., Ichise, H., Gola, A., Kabat, J., Lowekamp, B., Speranza...IBEX: An iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues...