...to isolate, multiply, or express the insert they carry in the target cell. These vectors allow you to test...approach of an autonomously replicating vector that carries either a wild-type version of YGOI to rescue a ...a microscope, we offer a collection of vectors carrying several fusion proteins. Pick your promoter, selection...can be expressed in fly culture cells like S2 and carry the metallothionein promoter (e.g. pMT-puro). Worm...

...field that you may want to work on in your future career, so the research topic will be more important. ...advisor that will support your growth in your science career. Will you be given opportunities to learn and practice...the PI support this path and help you pursue any career alternative? Listen to our podcast interview with...that will be a lifelong partner in developing your career in science.  Read other posts in our Science Mentors...

...patients or caregivers affected by the disease in question who are involved in online patient/caregiver communities...socioeconomic and/or racial/ethnic background, patient or caregiver status, geographic location, sense of humor, media...Activity Example audience  Talking about your career as a scientist underrepresented minorities in ...

... prototype to gain any real traction.  This can carry a heavy price tag, especially if you're working ... not value your potential contribution to the healthcare landscape.  You will find an extra layer of difficulty...name (P-H-D).  Finally, having spent your entire career in science, you are likely lacking the necessary...milabinc.com       Additional Resources Find more career posts on the Addgene blog Read our recent post ...

...mentors and, perhaps, asked someone to support your career development as your mentor. How do you make the...that you can reap rewards in the form of reaching career development goals. Check out Joanne's Reddit AMA...together and chatting with another person about their career path is a great start to a mentoring relationship...actionable steps (e.g. Meet 10 PhDs with interesting careers, find a summer internship, etc.). It is helpful...

... of researchers special: 1. Stem cell scientists care deeply about ethics Ethics must play a prominent...at scientific conferences. In my nearly 20-year career in science, I have never attended a scientific ...Researcher Darrell Kotton 2. Stem cell scientists care about patients As any grad student who has spent...alone takes nearly a year. Addgene depositor Juan Carlos Izpsua Belmonte said it took the joint effort of...

...Schwiesow A leading cause of death worldwide, cardiovascular disease is driven by atherogenic lipoproteins...been shown to have a strong correlation with cardiovascular disease and indirect measurement of ApoB-LPs...concentration, Steven Farber and colleagues at the Carnegie Institute for Science have developed the LipoGlo...to aid in the understanding and treatment of cardiovascular disease. Find the LipoGlo plasmids at Addgene...

... a lab that will be a good fit for you and your career ambitions. First, how does one stand out amongst...interview process. What if I want a non-academic career path?  If you are considering this, make sure to...will want in year 6. Have lab alumni taken diverse career paths? Ask other lab members how the advisor treats...inquiries. If you know you would like to head for a career in pharma or biotech, this is one case where the...

...application to assess your eligibility, interest, and career fit. Most internships share a common set of criteria...potential, and drive. Read the associated prompt carefully, plan your response, and let your inner wordsmith...applying to several opportunities. In an age of scarcity, the rational human being will tend to clinch ... Check out other tools and resources for early career researchers Applying for jobs? Check out these ...

...materials to specific destinations, haul a variety of cargo, and even handle a variety of terrains. They are...infection) and delivering a genetic payload. This cargo is typically their own genome, but this step can...cell) can be tuned so that the copies of library cargo delivered are controllable. For many screening applications...essential to deliver a single copy of your library cargo to isolate the effects of each variable being manipulated...

...Regenerative Biology is to understand the basis for cardiovascular and metabolic human diseases. They do that ...efficiently generate human stem cells edited to carry mutant versions of 15 different disease-associated... target a large number of genes involved in cardiovascular and metabolic diseases. We are knocking out... type relevant to the disease in question – cardiomyocytes, adipocytes or neurons, for instance – to see...

... about a Variety of Science Careers Resources on Addgene.org Find Career Opportunities at Addgene   ... - securing higher salaries. This decision will carry over into other aspects of postdocs’ lives. We know...plans and budgets working around issues such as childcare using money from their increased salaries. In ...

...Although it goes without saying that one should take care whenever doing any type of DNA or RNA purification...purification to avoid contamination, take extra care when performing RNA extraction. RNA is inherently not as ...problem with no-kit RNA extraction methods is the carryover of DNA that can potentially complicate results...extracting, to only take the aqueous layer to avoid carryover of DNA from the bottom, organic layer. Another...

...that’s an important soft skill to carry into the next phase of your career.  Happy defining! Additional...other transferable skills Find all of our science career related blog posts Read our science communication...communication blog posts Resources on Addgene.org Find careers at Addgene ...

... the Institutions” promotes gender equality in careers through structural change in research organizations...hormone therapy resulted in 76,000 fewer cases of cardiovascular disease, 126,000 fewer breast cancers, and ...fractures. The overall economic savings to the US healthcare system was $35.2 billion (or $140 per $1 invested...Extracurricular Activities for a Strong Science Career Path Additional Resources Check Out Vidoes of...

... on various applications throughout my graduate career, and helped receive funding towards my PhD project...Empowers the writer, And offers insight into a career as a Professor. Step 1: Your PI forwards you...criteria, and award administration information. Carefully read over all parts until all aspects are understood...Grant Application" by Vid Mohan-Ram in Science Careers "Beginnings - How to write your first grant proposal...

...2010 Yuhai Cui Red mScarlet Split-wrmScarlet variant of mScarlet Split-wrmScarlet and split-sfGFP: tools...

... deletion strains with overexpression plasmids carrying MET/Met genes from either S. pombe or S. cerevisiae...uracil, but contain methionine. (This allows cells carrying nonfunctional as well as functional MET genes ...only occur when galactose replaces glucose as the carbon source. Student results have shown that most, but...Basic Lab Techniques High School Students Explore Careers at Addgene Resources on Addgene.org Find Yeast...

...However, until recently safe delivery of nucleic acid cargo (DNA or RNA) to target cells has been challenging...serious adverse effects, and their immunogenic or carcinogenic profiles have limited their usability in clinical...It is an icosahedral, non-enveloped virus that carries a small, approximately 4.7 kb single stranded genome...single dose of subretinal administration of rAAV2 carrying the human gene RPE65, patients with clinical diagnosis...

... continue to shape the sector throughout their careers (McDowell et al, F1000Research, 2014; Dolan, F100Research...established, as they embark on or continue their careers in science-related fields. In particular, two recent... students in areas such as teaching salaries, healthcare access, and benefits; however, this may come ...Affairs at ASBMB Adam Fagen, PhD - Director, Early Career Scientist Segment at AAAS Marnie Gelbart, PhD -...