We narrowed to 465 results for: %s

A recent study shows ancestral bias in CRISPR guide libraries, leading to skewed cancer dependency results, particularly in cell lines with African ancestry.

Find plasmid tools for COVID-19 research expressing viral open reading frames, host factors, and tools for detecting SARS-CoV-2 and generating pseudoviruses.

Should you codon optimize your gene or not? Here, we discuss several reasons for and against codon optimization.

Part five of our transferable skills guide: Learn the basics of managing team from Addgene Senior Research Scientist Karen Guerin.

The yeast two hybrid (Y2H) assay is useful for probing interactions between proteins. Learn more about the Y2H assay and how you can use it in this post.

Learn about the sources of spontaneous recombination and optimizing Cre-recombinase dependent AAV vector expression in vivo.

Everything you need to know about antibodies in this blog post! Get the facts about the types of antibodies and how they can be used in the lab.

Get the basics on RNA interference (RNAi), how it compares with CRISPR, and tools for RNAi studies in plants.

Practical advice for using CRISPRs: designing your gRNA and introducing it into cells.

Designing a CRISPR experiment in bacteira? Check out these tools for genome engineering, CRISPRa, CRISPRi, base editing, and microbiome engineering.

Overview of Addgene's antibody production methods and quality control processes for purified recombinant antibodies.

Addgene has a growing collection of plasmids that are available to industry. Learn more about the available plasmids and how to order them.

Review of the most common types of affinity tags and there optimal usages.

This post covers loading controls and normalization of semi-quantitative western blots. We cover housekeeping proteins and total protein loading controls.

Learn how to verify indels, deletions, and homology directed repair (HDR) events in your CRISPR modified cell lines.

Pseudotyping is a method use to introduce a viral envelope protein from another virus to restrict or broaden host cell. Get the what, why, how of pseudotyping in this article.

There are many method to identify protein-protein interactions. Learn about different method of proximity labeling based on biotin-streptavidin interactions.

One-pot saturation mutagenesis can quickly generate libraries of mutant plasmids for phenotypic screening. Learn more from SciComm intern Beth Kenkel.

With this NIH's BRAIN Initiative Grant, Addgene will provide ready-to-use recombinant antibodies and will build a crowdsourced antibody Data Hub.

Find tips for hosting a great virtual lab meeting with ideas for online collaboration and space for brainstorming and discussion.