We narrowed to 430 results for: %s

...within 24 hours. References 1. Murphy BM, Swarts S, Mueller BM, van der Geer P, Manning MC, Fitchmun ...Agbandje-McKenna M, Cleaver BD, Clément N, Byrne BJ, Zolotukhin S. A simplified purification protocol for recombinant...

...inhibit type II CRISPR systems including the popular S. pyogenes Cas9 (Rauch et al., 2017, Hynes et al., ...DNA. What’s great about CASANOVA? It works with the S. pyogenes Cas9, dCas9-effector fusions, and xCas9,...

... Addgene!) pX330-U6-Chimeric_BB-CBh-hSpCas9 - The S. pyogenes Cas9 (SpCas9) has been widely popular in...Tet-pLKO-puro. lentiGuide-Puro - This plasmid expresses the S. pyogenes CRISPR chimeric RNA element with customizable...

...question(s). This precautionary step increases the likelihood of acceptance to the internship(s) you are...

...doi.org/10.1101/2024.12.16.628673 Zhou, S., Zhu, Q., Eom, M., Fang, S., et al. (2025). A sensitive soma-localized...PinkyCaMP plasmids and FRCaMPi plasmids! Fink, R., Imai, S., et al. (2024). PinkyCaMP a mScarlet-based calcium...

...Yamamoto, H., Hirasawa, A., Ennishi, D., Tomida, S., Toyooka, S., & Otsuka, M. (2025). Genomic Differences ...Peach, C. J., Kilpatrick, L. E., Woolard, J., & Hill, S. J. (2021). Use of NanoBiT and NanoBRET to monitor...

...tetracycline-responsive promoters. Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5547-51. doi: 10.1073/pnas.89.12.5547...: 27730575; PMCID: PMC5270762. Wendler, F., Park, S., Hill, C. et al. A LexAop > UAS > QUAS trimeric plasmid... the trp and lac promoters.” Proc Natl Acad Sci U S A. 80(1) (1983): 21-5. PubMed PMID: 6337371. PubMed...

...McAnally JR, Li H, Sanchez-Ortiz E, Bhattacharyya S, Shelton JM, Bassel-Duby R, Olson EN. Postnatal genome...Ousterout DG, Thakore PI, Moreb EA, Rivera RM, Madhavan S, Pan X, Ran FA, Yan WX, Asokan A, Zhang F, Duan D,.... pii: aad5143. PubMed PMID: 26721684. 4. Reardon S. Gene-editing summit supports some research in human...

...Springer Science+Business Media New York 2014Bustin S; Huggett J. qPCR primer design revisited. Biomolecular...Methods 283:291–306.Panina, Y., Germond, A., Masui, S. et al. Validation of Common Housekeeping Genes as....002.Teo IA; Choi JW;  Morlese J; Taylor G; Shaun S. LightCycler qPCR optimisation for low copy number...Nucleic Acids Res. 31:e122.Thelwell N; Millington S; Solinas A; Booth J; Brown T. Mode of action and application...

...applied to create photoswitchable spCas9, dCas9, and S. aureus Cas9 (saCas9) proteins, showing the versatility...Bubeck F, Hoffmann MD, Harteveld Z, Aschenbrenner S, Bietz A, Waldhauer MC, Börner K, Fakhiri J, Schmelas....201900788 Nihongaki Y, Furuhata Y, Otabe T, Hasegawa S, Yoshimoto K, Sato M (2017) CRISPR–Cas9-based photoactivatable...://doi.org/10.1038/nbt.3245 Nihongaki Y, Yamamoto S, Kawano F, Suzuki H, Sato M (2015) CRISPR-Cas9-based...

...chain, called a heavy chain antibody (Muyldermans S., 2013). They have the unique distinction of being... Methods. 10.1016/j.ymeth.2018.09.005 Muyldermans S. (2013). Nanobodies: natural single-domain antibodies...

...References Armbruster BN, Li X, Pausch MH, Herlitze S, Roth BL (2007) Evolving the lock to fit the key to...Smith Y (2019) Ultrastructural localization of DREADD s in monkeys. Eur J Neurosci 50:2801–2813 . https://...

...tools.   References and Resources References Pillay, S., et al. "An essential receptor for adeno-associated...2016. 501-507. PubMed PMID: 26427452. Issa, Shaza S., et al. “Various AAV Serotypes and Their Applications...

...with gRNA 1 to conduct microinjection experiments in S phase zygotes at 18 hr post fertilization. They observed...cells are needed for proper development. Ma et al.’s experiments showed a 3% mosaic rate, and this rate...

...process the array into functional crRNAs. Pro-Tip Cpf1’s ability to process its own pre-crRNA arrays simplifies...multiplexing: Transfection, lentivirus, and AAV Cpf1’s ability to process its own pre-crRNA arrays eliminates...

...Despite the success of Ma et al.’s approach, fluorescently labeled Cas9’s come with a number of limitations...

... can improve the specificity of Cas9 derived from S. aureus. This means you can likely apply similar rational...across a variety of genomic sites. Knowledge of Cas9’s dynamic structure leads to the development of HypaCas9...

...Expression System Activity Serotype PI 51509 AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE Syn-driven, Cre-dependent...

...PMCID: PMC2693335. Williams JA, Luke J, Langtry S, Anderson S, Hodgson CP, Carnes AE. Generic plasmid DNA ...circular DNA forms in bacteria. Proc Natl Acad Sci U S A. 1968 Dec;61(4):1406-13. doi: 10.1073/pnas.61.4.1406...

...002215549804600813 Erickson, P. A., Lewis, G. P., & Fisher, S. K. (1993). Chapter 15 Postembedding Immunocytochemical...-1  Manning, C. F., Bundros, A. M., & Trimmer, J. S. (2012). Benefits and Pitfalls of Secondary Antibodies...