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  1. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...PMCID: PMC2693335. Williams JA, Luke J, Langtry S, Anderson S, Hodgson CP, Carnes AE. Generic plasmid DNA ...circular DNA forms in bacteria. Proc Natl Acad Sci U S A. 1968 Dec;61(4):1406-13. doi: 10.1073/pnas.61.4.1406...

  3. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...species of yeast can be used for protein expression, S. cerevisiae, is the most reliable and frequently used...organism in genetics and biochemistry. When using S. cerevisiae, researchers often place recombinant proteins...

  4. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...002215549804600813 Erickson, P. A., Lewis, G. P., & Fisher, S. K. (1993). Chapter 15 Postembedding Immunocytochemical...-1  Manning, C. F., Bundros, A. M., & Trimmer, J. S. (2012). Benefits and Pitfalls of Secondary Antibodies...

  5. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...by secondary structure. It is thought that poly(A)s are used to control the cellular concentration of ... end formation. Cell. 1987. PMID: 3568131.  Hager S, Frame FM, Collins AT, Burns JE, Maitland NJ. An Internal...

  6. AAV Molecular Tools

    Type
    Collection
    ...Expression System Activity Serotype PI 51509 AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE Syn-driven, Cre-dependent...

  7. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Editing Species Human Mouse Fly Yeast T. gondii E. coli S. pneumoniae M. tuberculosis M. smegmatis Green monkey...136986 Knockout Human Doench 3rd 2 40,964 Ingolia lab S cerevisiae CRISPRi v1 – barcodes 181005 161769 Inhibition...Pool for Streptococcus pneumoniae 170432 Knockout S. pneumoniae Veening N/A 1 1,499 MusCK Library 174196...

  8. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ... Wu Y, Liang D, Wang Y, Bai M, Tang W, Bao S, Yan Z, Li D, Li J. Cell Stem Cell . 2013. PubMed ...colonies using the Qiagen Miniprep Kit. Zhang S, Cahalan MD. J. Vis. Exp . 2007. PubMed . Transformation... Gordanpour A, Nam RK, Sugar L, Bacopulos S, Seth A. J. Vis. Exp . 2012. PubMed . Western blotting...

  10. Common Injection Routes in Mice

    Type
    Blog Post
    ... https://doi.org/10.4236/jbm.2020.89011 Shimizu, S. S. (2004). Routes of administration. In H. Hedrich...

  13. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...discussing recent publications in a specific field(s). Most anyone who has spent any time in academia has...of critique and detail? Who is/are the facilitator(s) of the journal club? Where will the journal club ...

  15. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...dentate gyrus and olfactory bulb. Proc Natl Acad Sci U S A. 2013 Mar 19;110(12):E1152-61 Pubmed PMID 23487772...73. Pubmed PMID 22681690.  8, Wickersham IR, Finke S, Conzelmann KK, Callaway EM. Retrograde neuronal tracing...

  16. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...targeted sequence at the locus of interest. SpCas9’s 3’ NGG PAM occurs frequently in GC-rich genomes, but...AT-rich loci or genomes. Cas9s from other species, like S. aureus and N. meningitidis require different PAM ...

  17. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...components, and the accessibility of your target epitope(s). To achieve these goals, researchers commonly use...//doi.org/10.1177/33.8.3894499    Im K, Mareninov S, Diaz MFP, Yong WH (2018) An Introduction to Performing...

  18. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...TALENs and CRISPR. MMEJ is active during M and early S phases, when HDR is inactive, and the balance of NHEJ...still being explored. More broadly, Sakuma et al.’s use of MMEJ represents another strategy researchers...

  20. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...replication-defective virus particles with the SARS-CoV-2 spike (S) glycoprotein on the envelope surface. Pseudotyped...
Showing: 201 - 220 of 418 results