... However, even simple procedures like pouring a plate or running a gel have intricacies that aren’t always... soon, the Center for Open Science does host a platform for pre-registration wherein researchers can submit...

...materials and information. Many of our technology platforms like HaloTag® Fusion Protein, codon-optimized ...Machleidt, T., et al. (2015) NanoBRET-A novel BRET platform for the analysis of protein-protein interactions...

...associated with an elevated mutation burden. The CRISPR platform must be optimized to reduce off-targets and patient...Hopefully you can understand that depending on your platform, off-target effects should impact your experimental...

... the edit should be made HDR requires a repair template and displays reduced efficiency compared to NHEJ...

... present. The reaction takes place in a 96-well plate, where primary and secondary antibodies are used...Addgene blog: Read about another reagent sharing platform for antibodies, the Developmental Studies Hybridoma...

...the cell to use the deaminated DNA strand as a template. To do so, they used a Cas nickase, instead of...repairs the DNA using the U-containing strand as a template, copying the base edit. Figure 2: Cytidine...

...most commonly used is the Illumina NGS platform. This is the platform used by Addgene’s sequencing partner...of DNA, or templates, are spatially separated from each other. These attached DNA templates are then amplified... again producing ~1,000 copies of each template. Each template is then replicated using the modified bases...antibiotic(s) to add to your LB media or LB agar plates. Antibiotic Recommended Stock Concentration Recommended...the 4 nucleotides, a DNA polymerase enzyme, the template DNA to be copied, and a primer. A primer is a ...is a high-throughput, multi parallel sequencing platform that can generate sequencing data for up to 600...normal” bases in the reaction. All the sequenced templates are then aligned to each other to assemble the...

...genomic edits by copying from a DNA donor template . The donor template contains the desired sequence and is...linear dsDNA templates work better for large edits or insertions. When designing the donor template, incorporating...detection platform. Based on this non-specific cleavage, a Cas13a-based molecular detection platform, termed...pathway. HDR relies on copying DNA from a matching template to accurately repair or fill in the missing sequence...the break ends without the need for a homologous template. Both NHEJ and HDR have their uses, but we’ll ...binding site (PBS) and the desired edits on a RT template. The PBS base pairs to the 3’ flap, and the desired...driving mismatch repair using the edited strand as a template PE4/5 - improved PE2/PE3 to inhibit mismatch repair...

...S. pyogenes Kamoun pUC119-gRNA 52255 Plant PCR template none S. pyogenes Sheen pRGEB31 51295 Plant BsaI...

... Enzyme-linked immunosorbent assay (ELISA) is a plate-based assay that uses standard curves to quantify...Overview of application Coat antigens to the well of a plate, either by charge interactions (direct) or by a ...several wash steps. Add primary antibody to the plate. After incubation, wash to remove unbound antibody...Activate the conjugate and read the output via a plate reader. Special considerations The two most common...of interest is attached to the well of a 96-well plate. A conjugated primary antibody is used to bind to...be sorted (separated and deposited into tubes or plate wells) by fluorescence activated cell sorting (FACS...

...whenever a new video is posted! If TikTok isn't your platform, all of our videos will also be shared on our ...

..., they break down the unique challenges of our platform and how we were able to address these issues using...

...week, we launched our new AAV Data Hub, an open platform for scientists using AAV to find and contribute...

...festive makeover today✂️❄️🦠thanks to the awesome templates by @CVRHutchinson @CVRinfo. Check them out: https...

...automated vial filling, accurate tube-labeling, quick plate pouring, and much more. We didn’t want to keep the...

...launching the AAV Data Hub. The Data Hub is an open platform for scientists using AAV to share practical information...

... most recent protocol video detailing Streaking Plates for Single Bacterial Colonies can be found below...

...tubes, hang up your pipettes, and seal up those plates – it’s time to turn your attention to decorating...

...biologist Tips for protein purification Replica plating Anti-CRISPR Retroviral vectors Career development...

...be used to cut the DNA sequence. With a repair template, CRISPR can be used to introduce a functional ...