... gRNAs can be expressed in a single vector, pCRISPRainbow-DONOR1, with dCas9 supplied by a separate vector...

... in gene expression readouts from assays such as qPCR or microarray. But, aside from facilitating the ...

...based on a split NanoLuc® have been used to assess GPCR-G protein interactions , protein dimerization , ... firefly luciferase and GFP James Wilson 106457 pCR3.1-Luc Firefly CMV Mammalian expression of firefly...

...Rhodopsin GEVIs Rhodopsin is composed of an opsin (a GPCR) linked to a chromophore and functions as a photoreceptor...

...viral vector production facilities frequently use qPCR with a standard curve to titer their viruses; food...

...cells.  While Addgene uses a physical titer (via ddPCR) to quality control our viral preps, we recommend...

... of the new viral preps from recent months: New GPCR activation-based norepinephrine (GRABNE) sensors...

...laboratories. In the case of the COVID-19 pandemic, qPCR can be used to diagnose the presence of SARS-CoV...

...Parkinson's Mark Cookson 29354 pCR8-DJ1-WT PARK7 Parkinson's Mark Cookson 29355 pCR8-DJ1-D149A PARK7 Parkinson's...Parkinson's Mark Cookson 29356 pCR8-DJ1-E64D PARK7 Parkinson's Mark Cookson 29357 pCR8-DJ1-M26I PARK7 Parkinson's...-LFA EIF2AK2 GAL VWM disease Harmit Malik 20123 PCRII-zSynC SNCB Lewy body dementia Aaron Gitler 20648...SOD1 GFP CMV ALS Elizabeth Fisher 26573 CaV2.1 (pCR3.1) CACNA1A CMV Spinocerebellar ataxia 8 Diane Lipscombe...

...and the reverse transcriptase activity assay uses qPCR to quantify viral RNA. All of these methods have...

...key, especially for G-protein-coupled receptors (GPCRs). Mutations in receptor sites can affect the fluorescence...

...used either with plasma membrane proteins (such as GPCRs, ion channels, or cell surface receptors) or with...

...RNAi sequences, confirmation of RNA knockdown by RT-qPCR, and potentially the need to study protein expression...

...eabq8173. Yulong Li Norepinephrine nLight red and green GPCR-based NE indicators Sensitive multicolor indicators...Tommaso Patriarchi Norepinephrine GRAB_NE family of GPCR activation-based NE sensors A Genetically Encoded...

...cCGCTTCAGCggtctGTGGACTCGa zebrafish similar to grp151 (or gpcr-2037) OZ535 and OZ536 OPEN OPEN aGCCATCATCaggttcaGGATGAGCCc...

...three-part fusion proteins - Planas and Santalucia Labs pCri System : Heterologous protein expression in E. coli...

...lab Cas9 species = S. pyogenes (PAM = NGG) 42875 pCRISPR BsaI E. coli, S. pneumoniae Kanamycin none, need...

...undergo quality control, including AAV titration by ddPCR, in vitro and (when possible) in vivo testing, and...

... is necessary for Cas enzymes to bind target DNA PCR P olymerase C hain R eaction; Used to amplify a specific...

...Q157TAG Plasmid 53225 MBP-MEK1 S218TAG/S222TAG ( aka. PCRT7 tetR pLtetO MBP-MEK1 XX Amp) Plasmid 68300 MBP-MEK1...