Addgene

We narrowed to 275 results for: nif

Showing: 181 - 200 of 275 results

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GCE4All: Making Genetic Code Expansion Accessible

Type

Blog Post

... technologies have idiosyncrasies presents a significant barrier for those wishing to use it as a tool...
Viral Vectors 101: The AAV Retrograde Serotype

Type

Blog Post

...originally written by Leila Haery in 2017 and significantly updated in 2023 by Jason Nasse. References...
Antibodies 101: ChIP

Type

Blog Post

...Homogenization for a tissue sample can also need significant optimization for the sample type and size.) It...
Hot Plasmids - February 2022

Type

Blog Post

...is highly specific in mammalian cells without significant off-target effects, yet small enough to be packaged...
Sweating the Small Stuff: Details in the Lab

Type

Blog Post

...you're trapped in a pattern of small errors that significantly impacts your experiments. What do you do when...
3 Challenges in Plant Synthetic Biology

Type

Blog Post

... limited understanding of plant biology is a significant technical obstacle to plant synthetic biology...
Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

Type

Blog Post

... Jennifer Tsang...
15 Years of Addgene: The Top 15 Plasmids

Type

Blog Post

... Jennifer Tsang...
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

Type

Blog Post

...created an improved RNAi vector that leads to significantly stronger phenotypes than standard RNAi protocols...
Developing a cold tolerant E. coli using specialized chaperones

Type

Blog Post

...enzymes implies that even a minute quantity can significantly reduce the operational time for a given process...
CasPEDIA: A Functional Classification of Cas Enzymes

Type

Blog Post

...solve the problem: CasPEDIA, a new resource from Jennifer Doudna’s lab at the Innovative Genomics Institute...
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

Type

Blog Post

...activity relative to Cas9. Since then, the Joung and Jennifer Doudna Labs used targeted mutagenesis to generate...
Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

Type

Blog Post

...efficiency; where shRNA treatment produced 500-900 significant off-targets, CasRx displayed zero. Unlike Cas9...
Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

Type

Blog Post

...result, how most of these “variants of unknown significance” (sometimes called “VUS”) function is unknown...
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

Type

Blog Post

...plasma membrane. Note that the use of the HA tag significantly improves the transport of hM4Di to the plasma...
RANbodies: Reporter Nanobody Fusions

Type

Blog Post

...antibody-containing hybridoma supernatant is only $40, significantly cheaper than commercially available antibodies...
Human Germline Editing Using CRISPR

Type

Blog Post

...biologists and non-biologists alike. Given the public significance of this research, we have the obligation to ...
Career Coaching for Scientists: Why and Where Do I Find One?

Type

Blog Post

... for PhDs and postdocs posted by Toronto-based Jennifer Polk of From PhD to Life. She runs great tweet...
5 Steps to Writing Grant Proposals for Grad Students

Type

Blog Post

...strategy The Research Strategy highlights i) the significance and innovation of the project and ii) how you...
Reproducibility for Everyone: Lessons from an Open Science Collaboration

Type

Blog Post

...learned, and to Sonali Roy, Magdalena Julkowska, and Jennifer Tsang for editing. Additional resources on the...

Showing: 181 - 200 of 275 results

Results per page:

GCE4All: Making Genetic Code Expansion Accessible

Viral Vectors 101: The AAV Retrograde Serotype

Antibodies 101: ChIP

Hot Plasmids - February 2022

Sweating the Small Stuff: Details in the Lab

3 Challenges in Plant Synthetic Biology

Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

15 Years of Addgene: The Top 15 Plasmids

Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

Developing a cold tolerant E. coli using specialized chaperones

CasPEDIA: A Functional Classification of Cas Enzymes

Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

RANbodies: Reporter Nanobody Fusions

Human Germline Editing Using CRISPR

Career Coaching for Scientists: Why and Where Do I Find One?

5 Steps to Writing Grant Proposals for Grad Students

Reproducibility for Everyone: Lessons from an Open Science Collaboration

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