We narrowed to 285 results for: nif

1. The MTA at Addgene

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   Blog Post

   ...There are many different flavors of MTAs, but the Uniform Biological Material Transfer Agreement (UBMTA) ...

2. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

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   Blog Post

   ...System in Drosophila : A Fly Geneticist’s Swiss Army Knife, Genesis 34, 1–15 (2002).PubMed PMID: 12324939. ...

3. Four Factors that Differentiate the Stem Cell Field

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   Blog Post

   ...meeting shared plenty of details, but they also uniformly stepped back to give a big picture perspective...

4. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

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   Blog Post

   ... Jennifer Tsang...

5. 15 Years of Addgene: The Top 15 Plasmids

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   Blog Post

   ... Jennifer Tsang...

6. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

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   Blog Post

   ...created an improved RNAi vector that leads to significantly stronger phenotypes than standard RNAi protocols...

7. GCE4All: Making Genetic Code Expansion Accessible

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   Blog Post

   ... technologies have idiosyncrasies presents a significant barrier for those wishing to use it as a tool...

8. Viral Vectors 101: The AAV Retrograde Serotype

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   Blog Post

   ...originally written by Leila Haery in 2017 and significantly updated in 2023 by Jason Nasse.  References...

9. Developing a cold tolerant E. coli using specialized chaperones

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   Blog Post

   ...enzymes implies that even a minute quantity can significantly reduce the operational time for a given process...

10. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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    Blog Post

    ...activity relative to Cas9. Since then, the Joung and Jennifer Doudna Labs used targeted mutagenesis to generate...

11. Plasmids 101: Screening Strategies Used in Plasmid Cloning

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    Blog Post

    ... Jennifer Tsang...

12. Antibodies 101: ChIP

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    Blog Post

    ...Homogenization for a tissue sample can also need significant optimization for the sample type and size.) It...

13. Hot Plasmids - February 2022

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    Blog Post

    ...is highly specific in mammalian cells without significant off-target effects, yet small enough to be packaged...

14. Sweating the Small Stuff: Details in the Lab

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    Blog Post

    ...you're trapped in a pattern of small errors that significantly impacts your experiments. What do you do when...

15. CasPEDIA: A Functional Classification of Cas Enzymes

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    Blog Post

    ...solve the problem: CasPEDIA, a new resource from Jennifer Doudna’s lab at the Innovative Genomics Institute...

16. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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    Blog Post

    ...efficiency; where shRNA treatment produced 500-900 significant off-targets, CasRx displayed zero. Unlike Cas9...

17. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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    Blog Post

    ...result, how most of these “variants of unknown significance” (sometimes called “VUS”) function is unknown...

18. Screening for Successful Genome Editing with Digital PCR

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    Blog Post

    ...let’s explain what kind of assays can be used to sniff out your desired mutations. The assays you will ...

19. 3 Challenges in Plant Synthetic Biology

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    Blog Post

    ... limited understanding of plant biology is a significant technical obstacle to plant synthetic biology...

20. Reproducibility for Everyone: Lessons from an Open Science Collaboration

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    Blog Post

    ...learned, and to Sonali Roy, Magdalena Julkowska, and Jennifer Tsang for editing. Additional resources on the...