...sites, will result in similar sized bands, thus making this simple digest less informative. This is particularly...

...culture. Protocol Prepare liquid LB. For example, to make 400 mL of LB, weigh out the following into a 500...

...new window) Video Link Description Related Page Making LB Agar Plates Create plates to culture bacteria...

...even one less run a week makes a big difference. Clean out freezers so you can make room and don’t have to..., check out my Recycling and Innovations posts. Making small changes in your daily habits can feel ineffective...sustainability problem, how can one little change make a difference? Individual changes to our behavior...wouldn’t leave your oven on all day to save time making dinner at night, so why leave your lab equipment...

...requires that the RNA be tagged. “This tool makes it possible to make the protein specific to any endogenous...PUF proteins! A newly available PUF Assembly Kit makes it possible to devise RNA binding proteins to hit...continue to evolve and improve, Abil says they will make those new plasmids available through Addgene, and...the final version,” she said. “If we learn how to make the library even better, we would be very happy ...

...This is something I was expecting when I set out to make my first CRISPR/Cas9 gene edit. I wanted to inactivate...articles (gasp). To my delight, not only was I able to make the edits without any reagent-grade endangered Martian...the different cells (Figure 3).  3. In terms of making the monoclonal line: I had never done it before...important results (for example, if you needed to make a figure with the data). Using acrylamide instead...agarose to get sharper bands. Conclusion Overall, making a human cell line with a gene knockout was straightforward...who you are, you could quickly be on your way to making new scientific observations. References 1. Rago...

... puncta in each cell. When making figures for a paper or presentation, make sure the images are accessible...want to look for them? Or maybe you just want to make some really beautiful images? Multiplex IF may be...different colors in one session – fast and easy! Just make sure your primary antibodies are all of different...considerations to keep in mind. Most importantly, make sure you are using primary antibodies from different... and dye meets your needs and budget, or you can make your own. For example, to label the rat brain sample...

...people to take a break from their everyday tasks and make time for special projects? We borrowed an idea from...great success at accomplishing “we-should-really-make-time-for-that” type projects. Cross-team “Random...communication between labs. Scientists are often willing to make time for free food, and these lunches would provide...between labs, and people work hard at Addgene to make plasmid sharing as easy as possible. However, I ...work to share time with the people around us. It makes the quality of our work better and keeps us smiling...

... or set of plasmids. We hope that these articles make it easier for you to find and use the plasmids you...for in vivo expression, and/or produce vectors to make mutant cell lines or transgenic animals.   Wall...To expand the capabilities of Gibson assembly and make a more modular platform, the Jacks lab generated...cumbersome to use in a high throughput manner or make it difficult to quantify interaction strengths. ...Cas9-based AID tagging Seven years ago, Masato Kanemaki's lab successfully used the plant-based auxin-inducible...introduction of auxin range from 10-20 minutes, making AID an appealing tool for studying essential proteins...described in their recent Cell Reports paper, the Kanemaki lab overcame this hurdle using CRISPR technology...

...smallest bacteria. The absence of a rigid cell wall makes them resistant to antibiotics and antibacterial ...interpretation. Also, the signals from the nuclear area make the detection of mycoplasmas difficult (Ligasová...contamination when you are working without gloves or masks. Make sure to change the gloves and masks regularly instead...line stock for mycoplasma contamination before use. Make sure there is no contamination present in the serum...disposable pipettes to avoid cross-contamination. Make sure you keep all the required equipment and reagents...Avoid long exposure of the cell culture to air and make sure to tighten the flask cap before placing them...

...this responsibility seriously. Every decision we make, every project we undertake and every service we... that our materials are affordable but enough to make us self-sustaining. With this unique model, we are...opposed to fundraising.   Aside from enabling us to make all decisions with the goal of  benefiting our scientific...ever curious what specifically drives the decision making process at Addgene, just look to our core values...curated, high-quality data and reagents Addgene makes it easy for scientists to deposit and request plasmids...

... science brick-by-brick”. Our mission is to help make science mainstream and to show people the very human...at these struggles will bring people together and make these daily challenges a little easier to overcome... As we started building, we thought – how can we make the connection between LEGOs and science more concrete...and sounds to synchronize with the images thereby making the videos more real. To give you an idea of the...amount of work, see how many pictures we take to make a walk cycle of one step. Challenges in the LEGO...

... an incredibly fast-moving field, and we want to make it easy for you to keep up with new developments...to update our web resources and plasmid pages to make them more useful and easy to navigate. We also updated.... Base Edit: Base editing plasmids allow you to make precise C:G->T:A or A:T->G:C edits in DNA without...Like RNA targeting systems, RNA editing does not make changes to the genome, thus providing an avenue ...CRISPR Genome Editing and The CRISPR Software Matchmaker Day 2: Homology directed repair: CRISPR 101: ...

... and then condensing that and making it accessible to students.” Making science approachable is often ...eScience Labs.  “We work with faculty members to make sure the courses encompass what they want to teach...onsite, Caitlin can visit the operations team to make sure “that everything that’s going into the kit ...non-profit Fred Hutchinson Cancer Research Center. And make sure to subscribe to the Career Channel of the blog...

... programs and it seems to me that most Addgenies make an effort to avoid plastic bags and to conserve ...conservation in the lab One stingy science project was to make my own glass milk for purifying cut plasmid DNA ...amount of wasteful packaging.  Turns out, one could make a lifetime supply for about $10 US dollars worth...accessed blogs on BitesizeBio is the protocol for making your own Taq polymerase by Outreach Scientist, ...founder, Melina Fan, recently shared our protocol for making competent cells to save time and money. My other...

...Resource Identifiers (RRIDs) for each antibody, making it easy for you to determine whether any given ...been, a community resource that supports both toolmakers and tool users. We encourage you to share your...resource. We are excited to work with all of you to make this the best and most accessible recombinant antibody... are some ways that you can participate: If you make recombinant antibodies or other recombinant affinity...get started.  If you have suggestions about how to make the resource better or ideas for new materials to...

...DNA, but they still rely on the host machinery to make additional copies. ori sequences are generally high...replication machinery room to come in and get busy making copies.     Types of origins of replication ...plasmid type in your cells at a time? Do you want to make a lot of your plasmid? Is the gene toxic in high..., so if you need two plasmids for an experiment, make sure they have "compatible" ori's. See the table...more copies with the addition of chloramphenicol – make sure your growth conditions aren't working against...

...Gearing explains how Cas9 homolog, Cpf1, may make it easier to make gene edits at new genomic locations. 6.... Why and Where Do I Find One? If you need help making a transition into a new job or maybe to an entirely...gene delivery, but have limited packaging capacity making them difficult to use with CRISPR as the cargo....Your Genome Edit Once you’ve used CRISPR/Cas9 to make a change to the genome, you need to check whether...

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...within the UV spectrum, while wavelengths over 700 nm make up the infrared (IR) spectrum. When photons are ...advantage of a brighter fluorophore? Strong brightness makes it easier to distinguish a signal from noise; therefore... together in the same color panel. In this case, make sure to compare the excitation/emission spectra ...www.bdbiosciences.com/en-us/resources/bd-spectrum-viewer.   To make life easier, always try to use fluorophores with...worry, there are strategies to resolve this issue. Make sure to check out our blog post on color compensation...and 2) BV421 is a very bright fluorophore, which makes it easier to detect the transgenic protein. We don't...