...primary cells. Although cell entry of a Cas9 ribonucleoprotein (RNP) was limited (likely due to its long,...

...eSpCas9 and SpCas9-HF1. Both AsCpf1 and LbCpf1 ribonucleoproteins (RNPs) failed to induce off-target editing...

...Schnütgen F, Doerflinger N, Calléja C, Wendling O, Chambon P, and Ghyselinck NB. A directional strategy for...

...before the event... Choose a moderator with a backbone. It is nice to have a subject area expert, but...

...digested vector backbone. This is accomplished by covalently connecting the sugar backbone of the two DNA..., the insert DNA is physically attached to the backbone and the complete plasmid can be transformed into..., it is important to treat the digested vector backbone with a phosphatase before performing the ligation...

... include ribosomes, RNA polymerase, tRNAs, ribonucleotides and amino acids. Cell-free expression systems...

...the dsDNA template to a point where the hydrogen bonds break between the base pairs. This results in the...

...Protein Timers Browse All Fluorescent Protein Empty Backbones Check Out Our Fluorescent Protein Guide ...

...diluted from 10X and containing both Hepes and bicarbonate) is brought to room temperature and placed in...

...what do you want to make?   References: Jerome Bonnet et al. "Amplifying genetic logic gates." Science...

...The plasmids listed here are provided as empty backbones that utilize Golden Gate cloning to insert the...desired guide RNA sequence by digestion of the backbone with BbsI followed by a quick ligation of annealed...and Sequence Derivative Sources Original pX458 backbone ( Addgene #48138 ) dCas9 + KRAB-MeCP2 ( Addgene...

...enCh IP technology using recombinant CRISPR ribonucleoproteins." Genes to Cells 21.4 (2016): 370-377. PubMed...

...immediate co-transformation with linearized vector backbone. DpnI digestion - 1 μl DpnI is added to the...

...Maleimide-labeling occurs when a maleimide group forms a covalent bond with a sulfhydryl group (–SH), such as that found...

...is irreversible — FP fragments become covalently bonded by associating with one another — which means it...

...extraction protocol) disrupts the DNA’s hydrogen bonds with water molecules and coaxes it into sticking...

.... S. cerevisiae) Plasmid Type: Choose an empty backbone if you intend to clone your own insert, or use...

...periplasmic space to be oxidized and form disulfide bonds (Frenzel et. al., 2013). If you’re using a mammalian...

...eSpCas9 and SpCas9-HF1. Both AsCpf1 and LbCpf1 ribonucleoproteins (RNPs) failed to induce off-target editing...

...such as Thioredoxin A (TrxA) that improve disulfide bond formation in order to help keep your protein soluble...