...Page Learn How We Use NGS Sequencing to Validate AAV Preps Learn More about Pooled CRISPR Libraries Resources...

...crowdsourced Data Hub (built off of the concept of our AAV Data Hub) to share further validation data with you...

...manipulations such as injection of precise titrations of AAV or heat-shocking the organism. The SPARC system uses...

... of a live animal. They started by delivering an AAV construct expressing channelrhodopsin 2 (ChR2), a...

...and to compare different Cas enzymes in all-in-one AAV delivery. While Fragmid developers were specifically...Fragmid toolkit to facilitate other lentivirus- and AAV-based studies. Unified MoClo Systems While many MoClo...

...plasmids used to produce AAV in the lab. Addgene’s AAV Guide will teach you about AAV components, serotypes...Serotypes Pseudotyping AAV Variants References AAV Components The small (4.8 kb) ssDNA AAV genome consists of... discussed below. Overview of AAV Plasmid System Common Uses of AAV AAV is commonly used in optogenetics... The mutant AAV-DJ8 displays the properties of AAV-DJ, but with enhanced brain uptake. AAV Variants As...Epithelium) AAV1, AAV2, AAV4, AAV5, AAV8 Skeletal Muscle AAV1, AAV6, AAV7, AAV8, AAV9 Return to Top AAV Pseudotyping...cells and is a key advantage of rAAV. AAV Serotypes Eleven serotypes of AAV have thus far been identified... CNS AAV1, AAV2, AAV4, AAV5, AAV8, AAV9 Heart AAV1, AAV8, AAV9 Kidney AAV2 Liver AAV7, AAV8, AAV9 Lung...

...elements for deliveries via adeno-associated virus (AAV) and lentivirus, demonstrating simultaneous editing...

...to control the CRISPR system activity.  Using an AAV vector may help with size limitations. In addition...

...plasmids alone. (And we expect that resources like our AAV Data Hub will help scientists save even more!) If...

...off-target effects, yet small enough to be packaged in AAV. A dCasMini-mediated adenine base editor allows for...

...Carrier Proteins (SLCs) from the RESOLUTE collection AAV Packaging from Addgene Parkinson's Research from ...

...Adenovirus Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook Adenoviruses are...

...been deposited to Addgene, including lentiviral and AAV expression vectors for certain variants. Klapoetke... infeasible. Finally, an adeno-associated viral (AAV) system enables greater spatio-temporal control over... either the Thy1 or CAG promoter, while Brainbow AAV is under control of the EF1a promoter. Livet et...

... 74307 pAAV-CMV-DIO-Magneto2.0-sNRPpA AAV 74306 pAAV-CMV-DIO-TRPV4-p2A-ferritin-sNRPpA AAV 74302 ...plasmids, which already includes dSaCas9-VPR in an AAV transfer vector for in-vivo expression and plasmids...Magneto2.0 in the mammalian brain after delivering it via AAV injection, finding that it could increase the activity...lines by inserting OsTIR1 into the "safe harbor" AAVS1 locus (a tet-inducible OsTIR1 plasmid is also available...

...it easier to package for therapeutic delivery by AAV, which has strict capacity limitations. New efforts...Science, 362(6416): 839-842 (2018). 10.1126/science.aav4294 Ozcan, Ahsen, Krajeski, Rohan, Ioannidi, Eleonara...

...effects on neuron activity (firing or inhibition). AAV preps from DREADD plasmids allow you to easily express...

...lentivirus, Adenovirus, and Adeno-associated virus (AAV). If you are wondering which virus fits you experiments...

...γ-Retrovirus Guide Biosafety Guide Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook γ-Retrovirus (gamma-retrovirus...

...they drive expression. Different AAV serotypes can be used to target AAV vectors to specific cells of interest...considered. For tissue specificity of AAV serotypes, see our AAV guide . FLEx Vectors are also used to...models. For viral injection, Adeno-associated virus (AAV) is a widely-used tool for achieving in vivo expression...of chemogenetic receptors, and a wide variety of AAV-encoding chemogenetics plasmids are available. Additionally...available. Browse our collection of Ready-to-use AAV Preparations of Chemogenetic Plasmids ! Table 3. ...expression Localization. Depending on the experiment, an AAV-encoded chemogenetic plasmid may need to be targeted...regions of a neuron. Cell-specific expression of AAV-delivered constructs can be controlled with cell-...

...plasmids were developed to replace helper viruses in AAV production systems. We’re just skimming the surface...