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  1. Evolution of Lab Techniques

    Type
    Blog Post
    ...papers or are brainstorming new technological innovations. Let’s take a look at three historical techniques...past. In the sequencing world, one up and coming innovation is nanopore-based DNA sequencing which reads ...
  2. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...19 testing methods.   Rapid detection of 2019 novel coronavirus SARS-CoV-2 using a CRISPR based DETECTR...for rapid, ultrasensitive and visual detection of novel coronavirus SARS-CoV-2 Sensitivity: 4.6-11 copies...
  3. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...are not easily reversible. Late last year, the Akhmanova Lab published a new optogenetic tool for localized...Grigoriev, I., Nijenhuis, W., Kapitein, L. C., & Akhmanova, A. (2022). Opto-katanin, an optogenetic tool ...
  4. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...capable of inducing the brightest fluorescence (Filonov et al., 2014). Broccoli is twice as bright as Spinach2...9.10 (2014): 2412-2420. PubMed PMID: 25101481. Filonov, Grigory S., et al. "Broccoli: rapid selection ...
  5. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...e.g. mScarlet) or infrared fluorescent proteins (Chernov et al., 2017) may improve detection. Since longer...24360272. PubMed Central PMCID: PMC3918502.  14. Chernov, Konstantin G., et al. "Near-infrared fluorescent...
  6. Viral Production

    Type
    Collection
    ...serotype-specific Cap genes, and a plasmid encoding adenoviral helper sequences. AAV preparations are purified...
  7. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...strategies for combating cancers. To facilitate innovative cancer research, the Reference Reagents Group...robust CRISPR activators We previously reported on a novel Cas9-based activator developed by the Church lab...-X identified both previously characterized and novel mutants. Hess et al. envision CRISPR-X as not only...
  8. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...substrate-linked PEG, biotin, or magnetic beads. In one innovative example, the Zhang lab used SNAP-tag to decorate...Bulleit, R. F., & Wood, K. V. (2008). HaloTag: A Novel Protein Labeling Technology for Cell Imaging and...
  9. Plan Your Experiment

    Type
    Collection
    ...DSBs): For small nucleotide edits that introduce a novel restriction site. The region of interest is PCR ...
  10. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...Delivering genes across the blood-brain barrier: LY6A, a novel cellular receptor for AAV-PHP.B capsids. PloS One...Sabeti, P. C. (2023). Systemic administration of novel engineered AAV capsids facilitates enhanced transgene...
  11. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    ...characteristics of retroviruses, lentiviruses, AAVs, and adenoviruses in this easy-to-use guide. Print out the guide...
Showing: 181 - 200 of 351 results