...readout. A common method of labeling proteins is to fuse them with a fluorescent reporter, such as GFP or... a transduction reporter. Note that eGFP is not fused to CD45, but is separately expressed. (The reason...

...must wait for the opsin to express and passively diffuse down the length of the fiber before stimulation...are not actively transported and must passively diffuse so, depending on the length of your projection,...

...published and documented by OMICtools. It must be confusing for a newcomer to decide on the right software...of ideas is what makes choosing the right tool confusing and it means that there is no one-size-fits-all...

...genetically-encoded nuclear Cry2 fusion can thus direct a CIB1-protein fusion to the nucleus upon light activiation... an optogenetics procedure. A channelrhodopsin, fused to mCherry, is expressed in neurons (red dots). ...point mutations, codon optimization, and chimeric fusion of domains from two different ChRs. These ChR variants... given protein, such as the Cre recombinase, is fused to Cry2 and the other half to CIB1, light-stimulated...-part transcription factors - Cry2 and CIB1 are fused to a transcriptional activation domain and DNA binding..., both LOV and Dronpa can control activity of a fused protein through allosteric interactions. Researchers...cryptochrome 2 (Cry2) from Arabidopsis thaliana is fused to truncated forms of cytosolic STIM1 to control...

...overhangs, or which are both blunt-ended, can then be fused together during a ligation reaction with T4 DNA ...different molecular biology techniques (such as fusing it with different tags, putting it under a variety...ready TOPO® vectors that has a DNA topoisomerase I fused to the 3’ end. The topoisomerase acts as a ligase...molecules using the 4 nucleotides and lastly DNA ligase fuses DNA strands together. In Gibson assembly DNA fragments.... Similar to Gibson, this method can efficiently fuse two (or more) fragments of dsDNA that have 30 or... of DNA that share no end homology can still be fused together in a seamless manner. To accomplish this... (or more) fragments of DNA that you would like fused along with custom ordered DNA oligos of 60-80 base...

...EF1alpha-GFPcre Cre-GFP fusion EF-1 alpha Mammalian Sauer 11923 pBS598 EF1alpha-EGFPcre Cre-EGFP fusion EF-1 alpha ...Cre-EGFP fusion EF-1 alpha Mammalian Sauer 11956 pBS594 promoterless EGFPcre Cre-EGFP fusion in a promoterless...CRE-GFP fusion none Mammalian Sauer 11960 pBS537 tet-hCMV-GFPcre tet inducible Cre-GFP fusion tet-hCMV... CAG-GFP/cre Cre-GFP fusion CAG Retroviral Gage 49056 AAV-GFP/Cre Cre-GFP fusion CMV AAV Gage 49111 pEMS1980...Cre fused to the human Estrogen Binding Domain (EBD) TDH3 Yeast van Leeuwen 64770 pSS146 Cre fused to ... mCherry-Cre fusion and Venus Elastase Zebrafish Stainier 84033 pHD157 mCherry-Cre fusion and Venus fabp10...pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 EGFP-Cre fusion hSyn AAV Wilson 105545 pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 EGFP-Cre fusion CMV AAV Wilson...

...nEF CAG E2 regulatory element Tag Fusion tags mCherry HA Non-fusion tags mCitrine EGFP dTomato Activity...Inhibition HA fusion none 2, 5 Richie 121539 pAAV SYN1 HA-hM3D(Gq) hM3D(Gq) - Activation HA fusion none 2, ...mCherry fusion none 5 Roth 50479 pAAV-GFAP-hM4D(Gi)-mCherry hM4D(Gi) - Inhibition mCherry fusion none 5...-hM3D(Gq)-mCherry hM3D(Gq) - Activation mCherry fusion Cre-dependent 1, 2, 5, 8, 9, rg*, PHPeB Roth 44362...-hM4D(Gi)-mCherry hM4D(Gi) - Inhibition mCherry fusion Cre-dependent 1, 2, 5, 8, 9, rg*, PHPeB Roth 65417...pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine hM3D(Gq) - Activation HA fusion and IRES mCitrine Cre-dependent 8 Roth 50455 pAAV-hSyn-DIO-HA-hM4D...pAAV-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine hM4D(Gi) - Inhibition HA fusion and IRES mCitrine Cre-dependent 8 Roth 50458 pAAV-hSyn-DIO-rM3D...

...VNT : An intracellular calcium sensor created by fusing NanoLuc® and Venus fluorophore to Troponin C Ca...Nano-lanterns , and Antares : Fluorescent proteins are fused to Renilla luciferase (Nano-lanterns) or NanoLuc...pHlash , pRSETb-pHlash : A pH sensor created by fusing a bright mutant of Renilla luciferase (Rluc8) through...Firefly/Renilla Creating ORF-Firefly luciferase gene fusions. Renilla luciferase under the control of a CMV ...ccdB-Nanoluc NanoLuc® Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko Taipale 87075 pLenti6.2...ccdB-Nanoluc NanoLuc® Creation of C-terminal Nanoluc fusions using Gateway cloning. Lentival. Mikko Taipale ...Nanoluc-ccdB NanoLuc® Creation of N-terminal Nanoluc fusions using Gateway cloning. Lentiviral. Mikko Taipale...

...interest fused to the C-terminus of SCFP3A Amber N1 Yellow Mammalian Express a gene of interest fused to the...interest fused to the C-terminus of Amber mVenus N1 Yellow Mammalian Express a gene of interest fused to the...protein-protein interactions where each protein is separately fused to a donor or acceptor molecule (also referred to...a protein where the donor and acceptor are both fused to the same protein (also referred to as intramolecular...following plasmids can be used to create a fluorescent fusion protein with your gene of interest and the listed...mCerulean N1 Cyan Mammalian Express a gene of interest fused to the N-terminus of monomeric Cerulean mCerulean...mCerulean C1 Cyan Mammalian Express a gene of interest fused to the C-terminus of monomeric Cerulean mTurquoise2...

...recombination Back to Top Epitope Tag or Fusion Protein Tags and fusion proteins are excellent tools for further...the sequence of your gene and of the fusion protein. Tag or Fusion Protein Common uses Representative...vector backbones curated by species, epitope tags/fusion protein, selectable marker, viral expression, and... by: Species-specific expression Epitope tag or fusion protein Selectable markers Viral expression and... vectors - Inducible expression, fusion proteins, and more Sandmeyer Lab...mt-GFP - Create myc-tag, GFP-tag fusions for transcription pCSDest - Easy cloning of ...the function of your favorite gene. For example, fusing your protein to an epitope tag, such as Flag...

...between the RNAP subunit α fusion protein in pBRα-β flap and the λCI fusion protein in pACλCI-β-flap results...interest are fused to AraC or a dominant negative AraC. Preferential association of AraC-fusion homodimers... expression of an N-terminally His-tagged TALEN fused to an E. coli codon optimized FokI endonuclease ...and expression of a C-terminally His-tagged TALEN fused to an E. coli codon optimized FokI endonuclease ...fluorescent proteins Various PIs Fluorescent protein fusion: contains empty vectors with N and C-terminal fluorescent...that can be used to create fluorescent protein fusions with your protein of interest. See an overview ... of interest once its appropriate cDNA has been fused to the MS2 binding sites within this plasmid. pZE31...

...CRISPR Blog Posts Base Edit Catalytically dead dCas9 fused to a cytidine deaminase protein becomes a specific...Marker Publication Prime Edit Cas9 H840A nickase fused to a reverse transcriptase (RT) is capable of installing...PI Publication Activate Catalytically dead dCas9 fused to a transcriptional activator peptide can increase...Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide like KRAB...targeted epigenetic modifications, researchers have fused catalytically dead dCas9 to epigenetic modifiers...Cas13a/C2c2 and Cas13b, target RNA rather than DNA. Fusing the catalytic domain of ADAR2(E488Q) adenosine ...Visualize A catalytically inactive Cas9 (dCas9) fused to a fluorescent protein (FP) can help visualize...

... is fused to GFP, you may want to fuse your protein to a red fluorescent protein (see this fusion protein...examining the overlap between your fusion protein and the marker fusion protein using fluorescence microscopy...Addgene has assembled a collection of fluorescent fusion proteins that are targeted to various organelles...organelles or subcellular structures. You should fuse your protein of interest with a fluorescent protein that...Membrane CARMIL2 BH domain tdTomato John Cooper *Fusions to other FPs can be found by searching the complete...Microtubules ensconsin mCherry William Bement *Fusions to other FPs can be found by searching the complete...Junctions Zonula Occludens-1 GFP Alan Fanning *Fusions to other FPs can be found by searching the complete...

...libraries. Hot Plasmid 2: Transcription factor fusions modulate iPSC reprogramming efficiency. Listen ...

...catalytically inactive, human codon-optimized Cas9-BFP fusion expression plasmid 44248 pgRNA-humanized A customizable... vector containing SFFV promoter, dCas9 that is fused to 2x NLS and tagBFP 46911 pHR-SFFV-dCas9-BFP-KRAB... vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domain 46912 pMSCV-...vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, VP64 and tagBFP 51023 pSLQ1658-dCas9-...Human expression vector containing dCas9 that is fused to 2x NLS and EGFP for CRISPR imaging 51024 pSLQ1651...vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, p65 activation domain and tagBFP 46914...Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS controlled by TDH3 promoter 46921 pTDH3-dCas9...

...Kit Description Depositing PI FusX TALEN Assembly System Contains 336 pFusX clones allowing for Golden Gate...for expression as TALE-LSD1 fusions. It was recently shown that TALE fusions with the histone demethylase...are important new tools for genome engineering. Fusions of transcription activator-like (TAL) effectors...Takashi Yamamoto This accessory pack contains modified pFUS array vectors, destination vectors and a reporter... Gate TALEN and TAL Effector Kit. These modified pFUS vectors can reduce the number of module plasmids...Visualization [TGV]). pTALYM3 and pTALYM4 contain TALE fused with mClover and mRuby2, respectively, and can be...

...you have a question about culturing iPS cells? Confused by which system to use for genome editing? Know...

...nucleases that cut or modify RNA as opposed to DNA. Fusing the nuclease to an adenosine deaminase can convert...

...monoclonal antibody cell line, myeloma cells are fused with activated B cells from the spleen of mice injected...

... dCas9 fused to an activator peptide activates gene expression. Interfere dCas9 alone, or fused to a repressor...single-strand DNA breaks. Prime Edit Cas nickase enzymes fused to reverse transcriptase install new genetic information...peptide, inhibits transcription. Epigenetics dCas9 fused to an epigenetic modifier alters chromatin structure...across multiple systems. RNA Editing Cas13 enzymes fused to deaminases can edit bases in RNA. Other Applications...powerful tool for genome-wide screening. Purify dCas9 fused to an epitope tag can be used to isolate a given...