Cloning Mammalian Cells with the Agarose Method
Type
Blog Post
...such as HEK and CHO do not seem to require this, while nearly all endocrine cells do. While not all of ...different densities, then dispose of the plates that do not appear to be yielding well-spaced clones. For...replicates of each clone in case of contamination. We do not normally use antibiotics.
Lastly, and perhaps...experience, cell clones picked using the agarose method do not differ grossly from those isolated using cloning...