...recognition by MMR machinery and improved editing efficiency. The lab also optimized the architecture and amino...Enhanced prime editing systems by manipulating MMR machinery In their Cell paper, the Liu Lab found that mismatch... developed a system in which the cellular MMR machinery is disrupted via a dominant negative mutation ...

...thanks to our guest bloggers Joachim Goedhart and Marieke Mastop! Joachim Goedhart is an assistant professor...This post was contributed by guest bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular...fluorescent probes. You can follow him on twitter: @joachimgoedhart.   Marieke Mastop is a PhD candidate at the...

...wrong, ask yourself, “What next step will help me achieve my purpose? Should I go over this slide again? ...the presentation, at which point you will have achieved your goal. Yay!   You’re only human When it comes... in front of a very confused group of visiting Chinese undergrads. You get nervous and freeze, or forget...your slide deck gets uploaded. Your mic emits screeching feedback. You’re muted on Zoom. Your video conferencing...

... Arnold’s lab and Viviana Gradinaru’s lab used machine learning to design new channelrhodopsins that are...channelrhodopsin that’s successful in vivo. Using machine-learning to overcome channelrhodopsin design challenges...challenges, the Arnold and Gradinaru Labs developed a machine-learning model to help them construct “designer...Robinson JE, Mackey ED, Gradinaru V, Arnold FH (2019) Machine learning-guided channelrhodopsin engineering enables...

...CRISPR-Cas9-assisted genome editing in Escherichia coli. Acta Biochimica et Biophysica Sinica. https://doi....a technique that utilizes phage recombination machinery to promote homologous recombination of linear ...resistance E. coli carrying the phage recombineering machinery are first electroporated with pCas9. Then, pCRISPR.../10.1038/nbt.3043 Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA (2013) Programmable...

...which uses a much shorter crRNA. Cas12-RNP-PAGE achieved high editing frequency (nearly 100% in some cases...method is fast! These editing frequencies can be achieved with a 30-minute incubation, even with multiplexed...best-performing variant, CAP-Mac, was effective at breaching the blood-brain barrier and delivering a transgene...as well? Our collection includes a mouse and a chimeric rabbit (original mouse Fab region with rabbit ...

...Relations Board to allow graduate students who work as teaching or research assistants to join unions. The topic...provide a better deal for students in areas such as teaching salaries, healthcare access, and benefits; however... Genetics Education Project Lisa Coleman, PhD - Chief Diversity Officer Harvard University  The symposium...research: recommendations from the Future of Research Chicago Symposium F1000Research, 2016. Kyle T. Dolan, Joseph...

... more than 10 modules often rely on two-step hierarchical approaches using different Type IIS restriction...Our work demonstrates that it is now possible to achieve 20+ fragment assemblies with both robust efficiency...Additional confirmation of accurate assemblies was achieved by the sequencing of plasmids isolated from blue...fragment lacI/lacZ cassette assembly was easily achievable with high levels of transformants and low backgrounds...

... efficiency and building prediction tools with machine learning (PRIDICT, PRIDICT2.0).   How to apply...chromatin-based prediction tool (ePRIDICT). In summary, machine learning-based prime editing prediction models ...computational biology. His expertise includes applying machine learning techniques to make sense of biology, with...B. van, Krauthammer, M., & Schwank, G. (2024). Machine learning prediction of prime editing efficiency...

... tag, and the endogenous cellular degradation machinery to function. The dTAG is the smallest tag reviewed... and widespread usage, a proteolysis-targeting chimera (PROTAC) was developed for HaloTag. PROTACs are...functions – there are antibodies to it and ways to achieve fluorescence with it. AID The auxin-inducible degron...441. doi: 10.1038/s41589-018-0021-8 Holland AJ, Fachinetti D, Han JS, Cleveland DW. Inducible, reversible...

...level in vitro. In vivo monitoring has also been achieved with these sensors, but further optimization is...cell level as they are in vitro. Factors like photobleaching, lack of red-shifted indicators, and brightness...electrochromic FRET capable GEVIs, with rhodopsin quenching the added fluor for this two-part fluorescent ...voltage-dependent changes in rhodopsin modulate the degree of quenching of the fluor via FRET. This system is particularly...

...enabling microscopes to visualize the cellular architecture of whole tissues in 3D with unprecedented detail... through the specimen, which often leads to photobleaching.  Furthermore, point-by-point scanning along...focus light. Therefore, there is much less photo-bleaching or photo-damage than in conventional laser scanning...1007-1009. Pubmed. H.-U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C.P. Mauch,  K. Deininger, et al...

... E., Nguyen, J., … Chiu, C. Y. (2023). Adeno-associated virus type 2 in US children with acute severe ...three (Servellita et al., 2023). Normally when children have acute viral hepatitis it is caused by the...noting that it is possible to design a vector that achieves persistent transgene expression in certain cell...

...antibody production is Komagataella phaffii (a.k.a  Pichia pastoris) because it secretes fewer of its own ...include Human Embryonic Kidney (HEK) cells and Chinese Hamster Ovary (CHO) cells. (L'Abbé et al., 2018...-8730-6_1. PMID: 30242676. Frenzel A, Hust M, Schirrmann T. Expression of recombinant antibodies. Front....org/10.1016/j.cocis.2014.10.002. Randolph TW, Schiltz E, Sederstrom D, Steinmann D, Mozziconacci O, Schöneich...

...the term ‘grant writer’, but make no mistake, researching, winning, and effectively managing grants involves...development, certification, degrees, foundations, coaching and even Twitter Chats dedicated to the Grant ..., e.g. education, poverty, environment, early childhood development, or community development? Do you ...consultants who offer grant training, mentoring and coaching services, including Smartegrants, to help those...

...transfer vectors based on whether or not they have a chimeric 5'LTR. Second-generation transfer plasmids have... while 3rd-generation transfer plasmids have a chimeric 5'LTR that includes a CMV or RSV promoter as well... well as a portion of the 5'LTR. Including a chimeric 5'LTR removes the requirement for the HIV Tat protein...SIN is shorthand for self-inactivating, which is achieved by deleting a large portion of the 3'LTR in the...

...general, or anxiety associated with psychiatric diseases such as schizophrenia or bipolar disorder. In both ...found that downregulating expression of miR124a achieved a similar improvement in the forced swim test....the BDNF hypothesis of depression?." Molecular psychiatry 12.12 (2007): 1079. PubMed PMID: 17700574. Hare...

...create unmarked genetic modifications in the yeast Pichia pastoris. By combining both positive and counter...first described by studies of EcoRI and Bsp6I in Escherichia coli in 1995 when introduction of restriction-modification..., Hanna Engelberg-Kulka, and Gad Glaser. "An Escherichia coli chromosomal" addiction module" regulated...counter-selectable marker for unmarked genetic modification of Pichia pastoris." FEMS yeast research 9.4 (2009): 600-...

...recombinase have been developed. A common means to achieve ligand control is to fuse a mutant estrogen receptor...be transfected by in utero electroporation. To achieve sparse labeling of individual neurons one could...Central PMCID: PMC41617. 7. Metzger, D, J Clifford, H Chiba, and P Chambon. 1995. “Conditional Site-Specific...Recombination in Mammalian Cells Using a Ligand-Dependent Chimeric Cre Recombinase.” Proc Natl Acad Sci U S A 92(...

... cell lines that do not express these sugars. (Takeuchi et al., 1997). The alphaGal sugars end up in the...withstand ultracentrifugation which helps scientists achieve a high titer (reviewed in Joglekar and Sandoval... can bind and transduce many cell types before reaching the target cells. VSV-G is also toxic at high ...832 . https://doi.org/10.1182/blood-2001-11-0042 Takeuchi Y, Liong SH, Bieniasz PD, Jäger U, Porter CD, ...