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Considering purifying a protein to study it? Learn all about the polyhistidine tag, or HIS, in this blog post!

GPCRs are receptors that respond to stimuli and elicit cellular responses. Learn about the parts of GPCRs, how they work, and tools to study them.

Find tips for working with RNA and simple protocols for extracting RNA without using a kit

Learn how you can use RaPID to detect RNA-protein interactions and its applications in infectious and human genetic diseases.

This post describes CRISPR-derived base editors that catalyze base transversions, such as purines like A or G to pyrimidines like T or C.

Optogenetics aren't just for neuroscientists! In this post, we review optogenetic tools for cytoskeleton and membrane control in cells.

Dr. Michael Koeris is cofounder Biotech startup, Sample 6, and a member of the Addgene Board. Listen to his journey from grad student to entrepreneur.

Browse Addgene's COVID-19 collection of SARS-CoV-2 spike plasmids, packaging plasmids, and reporter plasmids for viral pseudotyping with SARS-CoV-2 spike protein.

This protocol describes how to generate a monoclonal cell line from a polyclonal pool of stable cells.

This protocol describes how to affinity purify recombinant antibodies from cell culture supernatant using Protein A or Protein G columns.

Learn about the csRNP epitope tagging method - a new and efficient way to tag endogenous genes using CRISPR.

Plasmid-encoded antibodies offers many advantages over other types of antibodies. Learn about how recombinant antibodies are made.

Discover the tools you need to perform plant genome engineering with CRISPR.

Summary of degron tags along with their applications, advantages, and several commonly used tags.

An overview of split fluorescent proteins and a highlight of some of the most popular and newest ones available on Addgene.

an overview of qPCR and ddPCR copy number variant assays.

Recent highlights from Addgene's collection include tools to study SARS-CoV-2 antibody escape, antibodies for aminoacyl tRNA synthetases, and plasmids for generating nanocompartments for yeast bioproduction.

Learn some best practices to keep your mammalian cell lines free of contaminants and your experiments running smoothly.

Ready-to-use retrograde AAV available from Addgene's viral service. Retrograde construct from Alla Karpova at Janelia Research Institute.

Check out the latest Hot Plasmids, viral vectors, and antibodies in the Addgene repository!