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Showing: 181 - 200 of 440 results

  1. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...Renaud J-B, Schneider-Maunoury S, Shkumatava A, Teboul L, Kent J, Joly J-S, Concordet J-P. Evaluation of...e58. https://doi.org/10.1093/nar/gky164 Cancellieri S, Canver MC, Bombieri N, Giugno R, Pinello L. CRISPRitz...

  2. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...this. 2) When your gene of interest has a BbvCI site(s). If so, the nicking mutagenesis protocol will still...still work if the orientation of the BbvCI site(s) of the gene are in the same orientation as those in ...site directed mutagenesis to remove the BbvCI site(s). Overall, the key features of the one-pot saturation...

  5. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...that can be imaged with the optics on the microscope(s) you intend to use. An accurate determination of whether...protein that is supposed to be extremely bright. In S. cerevisiae, we've tested a number of green and red...

  6. Technical Design of a Western Blot

    Type
    Blog Post
    ... incubation temperature for your specific protein(s) of interest.  Determining linear detection range ...assay or BCA assay. Be sure to aliquot your sample(s) before adding in your denaturing and/or loading buffers...that covers a size range appropriate for the target(s) and transfer method. Membranes The two membrane ...your standard curve, your standard, and your sample(s), as you can only compare samples to a standard curve...using a protein stain like Coomassie Blue or Ponceau S. It is far less prone to error than normalizing to...

  7. Genetic Code Expansion

    Type
    Collection
    ...209744 pAB228v TrpRS S. cerevisiae Bacterial Ahmed Badran 209745 pAB228v6 TrpRS S. cerevisiae Bacterial...)RS Methanogenic archaeon S-(4-cyanopyridin-2-yl)-L-cysteine (CysCNP) and S-(4-cyanopyrimidin-2-yl)-L-...Chimeric p-iodo-L-phenylalanine Bacterial TAG Dieter S√∂ll 104069 pTECH-chAcK3RS(IPYE) AcK3RS Chimeric Nε-acetyl-L-lysine...Badran 209746 pAMC070n1a12 ScTrpRS, Lum1PylRS, MmPylRS S. cerevisiae, Lum1, M. mazei Bacterial Ahmed Badran...

  8. CRISPR Guide Update

    Type
    Blog Post
    ...blog PRIME Editing CRISPR 101: Cas9 vs the Other Cas(s) Progress Towards a PAM-free CRISPR...

  9. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...190500 Homer1L/S scFv [L113/130] L113/130 scFv 2a Homer1L/S Mouse Mouse 190501 Homer1L/S scFv [L113/130...Gamma2L/S scFv [N452/73] N452/73 scFv 2T GABA(A)R, Gamma2L/S Human Mouse 190556 GABA(A)R, Gamma2L/S scFv ... Human Mouse IgG2a 177456 Anti-Homer1L/S [L113/130R] Homer1L/S Mouse Mouse IgG2a 177457 Anti-Homer1L [...IgG2a 177553 Anti-GABA(A)R, Gamma2L/S [N452/73R] GABA(A)R, Gamma2L/S Human Mouse IgG2a 177554 Anti-GABA...N476/9R] ZIP3 Mouse Mouse IgG2a 206600 Anti-S-tag [N56/9R] S-tag Bovine Mouse IgG2a 206601 Anti-TrpC4 [...Human Mouse IgG2b 206636 Anti-Homer1L/S [L113/130R-2b] Homer1L/S Mouse Mouse IgG2b 206637 Anti-Homer1L... Human Mouse IgG1 206687 Anti-Homer1L/S [L113/130R-1] Homer1L/S Mouse Mouse IgG2a 206688 Anti-Homer1L ...

  10. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ...Trinklein ND, Thie H, Wilkinson IC, Tandon AK, Anderson S, Bladen CL, Jones B, Aldred SF, Bestagno M, Burrone... XY, Eng H-Y, Lane D, Lefranc M-P, Clark M, Dübel S (2018) When monoclonal antibodies are not monospecific...

  11. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...Proteins Find AAV Tools References Marvin, Jonathan S., et al. “An optimized fluorescent probe for visualizing...PubMed Central PMCID: PMC4469972 Marvin, Jonathan S., et al. “Stability, affinity and chromatic variants...

  13. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...controls as you get your assays up and running.  Ed.'s note: More questions on antibody validation? Check...availability References Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, Rodriguez...

  16. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ... Addgene!) pX330-U6-Chimeric_BB-CBh-hSpCas9 - The S. pyogenes Cas9 (SpCas9) has been widely popular in...Tet-pLKO-puro. lentiGuide-Puro - This plasmid expresses the S. pyogenes CRISPR chimeric RNA element with customizable...

  18. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ...within 24 hours. References 1. Murphy BM, Swarts S, Mueller BM, van der Geer P, Manning MC, Fitchmun ...Agbandje-McKenna M, Cleaver BD, Clément N, Byrne BJ, Zolotukhin S. A simplified purification protocol for recombinant...

  19. Summer SciComm: Preprints

    Type
    Blog Post
    ...https://doi.org/10.1101/2021.09.07.459259 Sarabipour S, Debat HJ, Emmott E, Burgess SJ, Schwessinger B, Hensel...10.1371/journal.pbio.3000151 Sever R, Roeder T, Hindle S, Sussman L, Black K-J, Argentine J, Manos W, Inglis...

  20. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    ...polymerase, which has 3′→5′ exonuclease activity. T4’s polymerase activity can be thwarted if no dNTPs are...doi: 10.1007/978-1-61779-564-0_5. PubMed. JBEI-J5’s guide to SLIC Resources at Addgene Ligation-Independent...
Showing: 181 - 200 of 440 results