...gene dosage of glucose dehydrogenase and generated a strain that could achieve glucose dehydrogenase expression...our metabolic engineering case study of N-acetylglucosamine biosynthesis, we constructed a strain library...rounds of transformation to complete the N-acetylglucosamine strain library construction in 8 days. To ...numbers. (D) The effect of gene dosage on N-acetylglucosamine concentration. Image from the Yang lab. ...

... 3 4 Preferred carbon source Glucose Glucose Glucose, methanol, glycerol Heterothallic...its sole source of carbon and energy (as well as glucose or glycerol). K. phaffii is only distantly related...

...chrysanthemi, Escherichia coli, Gluconacetobacter xylinus, Gluconobacter spp., Hyphomicrobium spp., Klebsiella...spp., Caulobacter spp., Enterobacteriaceae, Gluconacetobacter xylinus, Haemophilus influenzae, Hypomycrobium...Caulobacter crescentus, Escherichia coli, Gluconacetobacter xylinus, Paracoccous denitrificans, Pseudomonas...

...repression in the presence of glucose or by competitive binding of the anti-inducer fucose Weaker. Commonly found...promoters Inducible with galactose; repressible with glucose  Can be used independently or together. Regulated...

...I2 sites. Supplementing cell growth media with glucose decreases cAMP and represses pBad, decreasing promoter...better option since expression can be repressed by glucose. Temperature inducible promoters are also known...

...algae host for reproduction. Snail parasite Leucochloridium is a parasitic worm that infects a snail’s ...

...microtubule-like structures are bacteria related to Verrucomicrobia. PNAS 97, 1813–1817 (2000). PubMed PMID: 10660683...

... (e.g. tears, saliva, mucus) where it protects mucosal membranes. This isotype is most commonly a dimer...

...complementation should only occur when galactose replaces glucose as the carbon source. Student results have shown...

...containing physiological concentrations of salts and glucose. If we circled well-separated clones containing...

... two guides per gene. The library was grown in glucose minimal media with 100-fold excess of wild-type...

...lineage-control network that differentiates human IPSCs into glucose-sensitive insulin-secreting beta-like cells." Nature...

...like protein in the ER: Identity with the 78 kd glucose-regulated protein and immunoglobulin heavy chain...

... additional carbon sources such as glycerol or glucose (in moderation) may also serve to increase cell...

...and 5 g/L NaCl) or on LB-agar plates containing glucose and L-arabinose at 37oC for at least 12 hours, ...

...enzymes are essential for processes such as gluconeogenesis, lipogenesis, amino acid metabolism and energy...

... yeast. In the first 24 hours of culture, when glucose is abundant and ethanol concentrations are low,...

...20. Gary Yellen Glucose iGlucoSnFR biosensors for cytosolic glucose Looger Lab Glucose Sensor Plasmids...00301-9. Takashi Tsuboi Glucose iGlucoSnFR-TS biosensor for intracellular glucose Quantitative in vivo imaging...6(8):1441-1450. Robert Campbell Glucose FRET sensor to monitor glucose levels and transport (FLIPglu) ...Lab FLIPglu Plasmids Wolf Frommer Glucose FRET sensor to monitor glucose levels and transport (FLIIglu) ...Lab FLIIglu Plasmids Wolf Frommer Glucose Green fluorescent glucose indicators for living cells (Glifons...) Green Fluorescent Protein-Based Glucose Indicators Report Glucose Dynamics in Living Cells. Anal Chem...91(7):4821-4830. Tetsuya Kitaguchi Glucose Red fluorescent glucose indicators for living cells (Glifons...

.... This will include a discussion of the Human Leucocyte Differentiation Antigens (HCDM) workshop approach...

...truncated rat homologue of mouse prominin, is blood glucose-regulated and can up-regulate the expression of...