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Showing: 1 - 9 of 9 results

  1. AAV Production in HEK293 Cells

    Type
    Protocol
    ...maximize titer . DMEM high glucose, Corning 10-013-CV DMEM, low glucose (1 g/L) glucose, sodium pyruvate, Corning...DMEM low glucose + 1% v/v HI-FBS + 0.1 M sorbitol In our hands, we have found that a lower glucose, lower...such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat-inactivated FBS and 5 mL of ...Bicarbonate, 7.5 mL 1 M HEPES to 750 mL DMEM + 1 g/L glucose. 1 mg/mL polyethylenimine (PEI) solution: Dissolve...

  2. Fluorescence Titering Assay

    Type
    Protocol
    ...Inverted fluorescence microscope Reagents DMEM high glucose, Corning 10-013-CV L-alanyl-L-glutamine (or alternative...such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat-inactivated FBS and 5 mL of ...

  3. General Transfection

    Type
    Protocol
    ...controller Hazardous waste container Reagents DMEM high glucose, Corning 10-013-CV L-alanyl-L-glutamine (or alternative...such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat-inactivated FBS and 5 mL of ...

  4. Lentivirus Production

    Type
    Protocol
    ...Stir plate Magnetic Stir Bar Reagents DMEM high glucose, Corning 10-013-CV L-alanyl-L-glutamine (or alternative...such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat-inactivated FBS and 5 mL of...

  5. Colony Formation Titering Assay

    Type
    Protocol
    ...controller Hazardous waste container Reagents DMEM high glucose, Corning 10-013-CV L-alanyl-L-glutamine (or alternative...such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat inactivated FBS and 5 mL of ...

  6. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...controller Hazardous waste container Reagents DMEM high glucose, Corning 10-013-CV L-alanyl-L-glutamine (or alternative...such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat-inactivated FBS and 5mL of 100X...

  7. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...Reagents Polyclonal stable cell pool DMEM high glucose, Corning 10-013-CV L-alanyl-L-glutamine (or alternative...such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat-inactivated FBS and 5 mL of ...

  8. Lentivirus ddPCR Titration

    Type
    Protocol
    ...culture treated dish, VWR, 29442-042 DMEM, high glucose, pyruvate, Corning, 10-013-CV Heat-inactivated ...Reagent Preparation DMEM Complete: 500 mL DMEM, high glucose, pyruvate 55 mL heat inactivated premium grade,...
Showing: 1 - 9 of 9 results