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  1. Structural Genomics Consortium Plasmids

    Type
    Collection
    ...Hexahistidine tag with TEV cleavage, AmpR 26093 pET15-MHL EF456738 Hexahistidine tag with TEV cleavage, AmpR ...Hexahistidine tag with TEV cleavage, KanR 26105 pNIC-CTHF EF199844 Hexahistidine tag, FLAG tag with TEV cleavage, ...thioredoxin with TEV cleavage, KanR 26107 pNIC-ZB GU452710 Hexahistidine tag , Z-basic, TEV cleavage, AmpR... 26096 pET28-MHL EF456735 Hexahistidine tag with TEV cleavage, KanR 26098 pCW-LIC EF460848 No tag, double...26108 pFB-LIC-Bse EF199842 Hexahistidine tag with TEV cleavage, AmpR 26095 pFHMSP-LIC-N N/A Expression ...secreted proteins with N-terminal hexahistidine tag and TEV cleavage, Bac-to-Bac Expression System, AmpR 26100...secreted proteins with C-terminal hexahistidine tag and TEV cleavage, Bac-to-Bac Expression System, AmpR Related...

  2. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Gitler 29629 GST-TEV-FUS FUS TEV, GST T7 ALS Aaron Gitler 29630 GST-TEV-FUS-267-526aa FUS TEV, GST T7 ALS ...29631 GST-TEV-FUS-1-373aa FUS TEV, GST T7 ALS Aaron Gitler 29632 GST-TEV-FUS-1-413aa FUS TEV, GST T7 ALS...pGST2-GST-TEV-OPTN S177D S473D OPTN GST-TEV ALS Sascha Martens 187830 pFastBac_Dual_GST-TEV-EGFP-TBK1 ...pET-Duet1-His-TEV-mCherry-OPTN WT OPTN His, TEV, mCherry ALS Sascha Martens 190192 pET-Duet1-6xHis-TEV-OPTN OPTN...-GST-TEV-mCherry-TBK1 TBK1 mCherry, GST, Tev P10 ALS Sascha Martens 198037 pFastBac_Dual_GST-TEV-EGFP-...666) TBK1 GFP, GST, Tev P10 ALS Sascha Martens 198428 pGST2-GST-TEV-OPTN OPTN TEV, GST tac ALS Sascha ...PRKCG PRKCG His, TEV T7 Spinocerebellar ataxia 18 Nicola Burgess-Brown 39042 GAK GAK TEV, His T7 Parkinson's...

  3. Hot Plasmids - August 2020

    Type
    Blog Post
    ...of the TEV cleavage and improve utility of the system for use with low affinity variants of TEV. The new...evolution platform to generate TEV protease variants. Catalysis by TEV protease allows a transcription...catalytic efficiency of tobacco etch virus protease (TEV), a highly sequence-specific protease used to cleave...

  4. Plasmids 101: Protein tags

    Type
    Blog Post
    ...best optimized is the tobacco etch virus (TEV) protease. A TEV protease cleavage site is frequently placed...comprised of a calmodulin binding peptide (CBP), a TEV cleavage site (more on that in a moment), and 2 ProtA...cleavage reaction taking place between column runs. The TEV protease itself, with various mutations used to increase...with tags Protease Recognition site Notes TEV ENLYFQS Cleaves between the Gln and Ser residues ...

  5. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... TEV-His6-Flag for bacterial expression pET Flag TEV - N-terminal Flag-TEV for bacterial...bacterial expression vectors pET His6 TEV - N-terminal His6-TEV tag for bacterial expression pET...mammalian expression pET His6 GST TEV - N-terminal His6-GST-TEV for bacterial ...

  6. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...few of our proteins that lacked TEV. Although we were unable to cleave TEV within the protein we tested,...the AviTag. As a side note, we found that adding a TEV linker dramatically increased accessibility of the...

  7. Bacterial Expression Systems

    Type
    Collection
    ...(lactose/IPTG inducible) N terminal GST tag with TEV cleavage site to remove tag Nicola Burgess Brown ...pGEX expression vector with N-terminal GST tag and TEV cleavage site. Includes sites for LIC cloning, and...26106 T7 LacO (lactose/IPTG inducible) N terminal, TEV cleavable 6x-His tag Ophear Gileadi pET-28-a based...based expression vector containing N-terminal, TEV cleavable, 6x-His and thioredoxin tags. Other empty vectors... His, Strep, mOCR, MBP, with and without SUMO or TEV cleavage sites to remove tags Scott Gradia Arabinose... Strep, mOCR, MBP, GFP, with and without SUMO or TEV cleavage sites to remove tags. Scott Gradia Lactose...26092 T7-lacO (lactose/PTG inducible) C-terminal TEV cleavable 6x His Cheryl Arrowsmith Empty lactose/...

  11. Open Enzyme Collection

    Type
    Collection
    ...Addgene ID Plasmid Gene/insert 165509 pOpen-TEV protease TEV protease 165565 pOpen-RNAse A RNAse A 165575...

  17. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ... knock-out in your experiments? AAV-flex-taCasp3-TEVp from Nirao Shah's and Jim Well's lab induces cre-dependent...viral prep in serotype AAV5. Find AAV-flex-taCasp3-TEVp here The red-shifted channelrhodopsin (C1V1) targeting...

  18. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...are then washed, followed by antibody elution. Whatever method you choose, you may not be able to elute...time, reagents, and equipment available to you. Whatever you choose, you’ll want an accurate concentration...

  19. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...the transgenic genome will simply express from whatever promoters were included in the vector (or are ...instead get expression of the sensor, marker, or whatever other tool you chose to express. In the case ...

  20. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...and Chemistry activities from Quatr.us Foldscopes Steve Spangler Science The Fab Lab Middle school: supplementing...exciting, tumultuous time in scientific history. Whatever your reasons, let us know how it goes! Acknowledgements...
Showing: 1 - 20 of 80 results