...of the TEV cleavage and improve utility of the system for use with low affinity variants of TEV. The new...evolution platform to generate TEV protease variants. Catalysis by TEV protease allows a transcription...catalytic efficiency of tobacco etch virus protease (TEV), a highly sequence-specific protease used to cleave...

...best optimized is the tobacco etch virus (TEV) protease. A TEV protease cleavage site is frequently placed...comprised of a calmodulin binding peptide (CBP), a TEV cleavage site (more on that in a moment), and 2 ProtA...cleavage reaction taking place between column runs. The TEV protease itself, with various mutations used to increase...with tags Protease Recognition site Notes TEV ENLYFQS Cleaves between the Gln and Ser residues ...

...few of our proteins that lacked TEV. Although we were unable to cleave TEV within the protein we tested,...the AviTag. As a side note, we found that adding a TEV linker dramatically increased accessibility of the...

...disulfide bonds fold properly. If a TEV protease site is present, TEV can remove these tags from the purified...

...K5 linkers that are connected to a TEV protease cleavage site. TEV protease is a highly specific protease...

...recruits a beta-arrestin-TEV protease fusion to a receptor-transcription factor fusion. TEV protease cleave the...

...with each possible combination of protease site (TEV or PreScission), epitope tag (12xHis, 2xStrep, 3xFlag...

...optimized, untagged, cleavage sites (thrombin and TEV), and C-terminal tags (StrepII, 3xFLAG, 6xHIS, MBP...

...additional useful tags, including 3xFLAG epitopes, TEV and BioTag, which can be used in protein affinity...

...N-terminal tagging vectors (eg GFP, Flag, YFP, Strep, TEV-V5) with either blasticidin or puromycin selection...

... knock-out in your experiments? AAV-flex-taCasp3-TEVp from Nirao Shah's and Jim Well's lab induces cre-dependent...viral prep in serotype AAV5. Find AAV-flex-taCasp3-TEVp here The red-shifted channelrhodopsin (C1V1) targeting...

...are then washed, followed by antibody elution. Whatever method you choose, you may not be able to elute...time, reagents, and equipment available to you. Whatever you choose, you’ll want an accurate concentration...

...the transgenic genome will simply express from whatever promoters were included in the vector (or are ...instead get expression of the sensor, marker, or whatever other tool you chose to express. In the case ...

...and Chemistry activities from Quatr.us Foldscopes Steve Spangler Science The Fab Lab Middle school: supplementing...exciting, tumultuous time in scientific history. Whatever your reasons, let us know how it goes! Acknowledgements...

..., you can easily convert the citation style to whatever the journal requires. Using the (Last name, Year...woods for months at a time, so take advantage of whatever time you have. If you’re like me, you might be...

...repository 49:45 0 1:18:45 - Reagent Development - Steven C. Almo, Institute for Protein Innovation  1:18...aliquot pulled from a freezer. As you’ll see in Steve Almo’s talk about work at the Institute for Protein...

...contributed by guest blogger Pooran Dewari, a postdoc in Steve Pollard’s lab at the MRC Centre for Regenerative... genome editing! Pooran Dewari is a postdoc in Steve Pollard’s lab at the MRC Centre for Regenerative...

...Meltzer, J. C., Buzhdygan, T., Cheng, M. J., Sena-Esteves, M., Bennett, R. E., Sullivan, T. P., Razmpour,..., Allaway, K., Sakopoulos, S., Schneider, G., Stevenson, O., Vergara, J., Sharma, J., Zhang, Q., Franken...

...electronically or choose to auto-approve MTAs. Whatever your preference, we are here to make it fast and...

...manager may appear to be too busy to be receptive.   Whatever the reason, it is a fortunate and successful manager...