...Assembly standards define standard prefix and suffix sequences that make it easy to combine components... Assembly Standards Guide SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog...use of assembly standards – combinations of restriction sites which preserve a standard prefix and suffix.... BioBrick BioBrick-2 BglBrick Silver Standard Freiburg Standard BioBrick (RFC 10) Prefix: GAATTC GCGGCCGC...visit iGEM: BglBrick – Berkeley Standard (RFC 21) Back to Top Silver Standard (RFC 23) Prefix: GAATTC GCGGCCGC...more info, visit iGEM: Silver Standard (RFC 23) Back to Top Freiberg Standard (RFC 25) Prefix: GAATTC GCGGCCGC...following table provides information for the assembly standards most commonly used by part registries, including...

...interactions or conformation changes, as well as FRET standards....pair, the FRET efficiency strongly depends on the distance between the donor and acceptor molecules and can... can therefore be used to calculate the distances between the donor and acceptor. FRET is often used ...protein-protein interactions and a series of FRET standards . Pre-constructed fluorescent biosensors targeting...between ECFP and Venus Back to Top FRET Reference Standards The following plasmids have well-characterized... values and were developed to be used as FRET standards to calibrate the numerous and disparate methods...linker-Venus-6aa linker-Venus V5V Anisotropy/brightness standard consisting of two Venus fluorescent proteins connected...

... the constant region, and is shown in dark blue and dark orange. The constant region is a stable part ...antibody’s constant region - specifically, it is determined by a region called the “heavy chain constant region...techniques use a nuclear stain, such as DAPI, to identify individual cells. These stains also emit fluorescent...sequencing, and enables the variable region and constant regions of the antibody to be independently manipulated...Structure Figure 1: Structure of an antibody A standard antibody is made up of four protein chains: two...responsible for producing antibodies, can change the constant region, and therefore change the isotype of antibodies...capable of recognizing and binding epitopes on constant regions of other antibodies. This has useful research...

...-iCAP-PHP.eB PHP.eB AAV packaging plasmid (non-standard), expressing Rep2 and PHP.eB Cap controlled by...pUCmini-iCAP-PHP.S PHP.S AAV packaging plasmid (non-standard), expressing Rep2 and PHP.S Cap controlled by ...-iCAP-PHP.V1 PHP.V1 AAV packaging plasmid (non-standard), expressing Rep2 and PHP.V1 Cap controlled by...pUCmini-iCAP-AAV.CAP-B10 B10 AAV packaging plasmid (non-standard), expressing Rep2 and B10 Cap controlled by tTA-TRE...pUCmini-iCAP-AAV.CAP-B22 B22 AAV packaging plasmid (non-standard), expressing Rep2 and B22 Cap controlled by tTA-TRE...pUCmini-iCAP-AAV.MaCPNS1 MaCPNS1 AAV packaging plasmid (non-standard), expressing Rep2 and MaCPNS1 Cap controlled by...pUCmini-iCAP-AAV.MaCPNS2 MaCPNS2 AAV packaging plasmid (non-standard), expressing Rep2 and MaCPNS2 Cap controlled by...

...MGC110940, OPN SST somatostatin SMST SSTR1 somatostatin receptor 1 SRIF-2 SSTR2 somatostatin receptor 2 - SSTR5...immunoglobulin heavy constant alpha 2 (A2m marker) - IGHD immunoglobulin heavy constant delta FLJ00382, FLJ46727...IGHE immunoglobulin heavy constant epsilon IgE IGHG1 immunoglobulin heavy constant gamma 1 (G1m marker) -...immunoglobulin heavy constant gamma 2 (G2m marker) DKFZp686I04196 IGHG3 immunoglobulin heavy constant gamma 3 (G3m...immunoglobulin lambda constant 1 (Mcg marker) IGLC IGLC2 immunoglobulin lambda constant 2 (Kern-Oz- marker...immunoglobulin lambda constant 3 (Kern-Oz+ marker) IGLC IGLC6 immunoglobulin lambda constant 6 (Kern+Oz- marker...early stage tumors ARP, MANF, MGC142148, MGC142150 ARMETL1 arginine-rich, mutated in early stage tumors-like...

...is provided to the starter cell in trans , via delivery by an AAV. Since this starter cell now has all ...TVA and G-protein is delivered to the starter cell. (2) The starter cell now expresses TVA and G-protein... RAVB is delivered to the starter cell, and specifically targets the starter cell via the EnvA receptor...). Thus, G-deleted rabies can infect a single "starter" cell, but new virus cannot spread to any neighboring...can spread to cells that are projecting to the starter cell). Once in the secondarily infected cells that... the targeting of G-deleted rabies to specific starter cells, the tropism of this virus was modified so...receptor. RABV is able to replicate in the starter cell using the G-protein, which is supplied in trans . (...

...used to install the LSR Bxb1’s landing site into the genome at a desired locus, then Bxb1 installs the donor...including resistances to chemotherapy drugs and toxins, cell viability, and tumor metastasis. One of the...genome-wide screens. This guide will provide a basic understanding of CRISPR biology, introduce the various applications...applications of CRISPR, and help you get started using CRISPR in your own research. CRISPR Basics In this...target cleavage, while mismatches toward the 5′ end distal to the PAM often permit target cleavage. Generating... Cas9 orthologs are available. SaCas9 (from Staphylococcus aureus ) has a coding sequence ~1 kb shorter...addition of a structured motif to enhance pegRNA stability PEmax - optimized PE architecture; increased synergy...

...concentration is confirmed by Coomassie staining. For Coomassie staining, the sample is separated via a denaturing...alongside serial dilutions of a standard antibody of known concentration and stained with Coomassie blue. Protein...and a standard curve is generated. The final antibody concentration is calculated from the standard curve...by Addgene scientists. They are generated using standard methods individually optimized for each specific...opens in a new window) page. Storage and Shipping Stability Addgene does not recommend repeated freeze thaw... antibody catalog item is tested for shipping stability by incubating at 37 °C for 2 weeks, freezing at...recommended assays. If an antibody has passed stability testing, it may ship at room temperature. However...

...Once you have a clear understanding of your experimental goal, you are ready to start navigating the different...generate stable cell lines (for details, see Plasmids 101: Mammalian Vectors ) Transient or stable expression... gRNA & Cas9 Validate Results Publications Get Started CRISPR is a powerful system that enables researchers...section will provide a general framework to get you started using CRISPR in your research. Although we will...cell types (e.g. HEK293 cells), transfection with standard transfection reagents may be sufficient to express...details about lentivirus, see our Lentivirus Guide ) Stable, tunable expression of Cas9 and/or gRNA in a wide...compatible with smaller Cas enzymes) Transient or stable expression of SaCas9 and/or gRNA Infects dividing...

...Mouse IgG2a 114557 Anti-TRIP8b (constant) [N269/73R] TRIP8b (constant) Rat Mouse IgG2a 114558 Anti-Synaptotagmin...Mouse IgG2a 128635 Anti-Ankyrin-G (staining) [N106/43R] Ankyrin-G (staining) Human Mouse IgG2a 128636 Anti-LRRTM4...Rat Mouse IgG2a 140065 Anti-SCG10/Stathmin-2 [L5/1R] SCG10/Stathmin-2 Rat Mouse IgG2a 140066 Anti-Co-Rest...-Pan-Gamma-protocadherin-Constant [N159/5R] Pan-Gamma-protocadherin-Constant Mouse IgG2a 177504 Anti-LAR... Mouse IgG2a 177514 Anti-TRIP8b (constant) [N212/7R] TRIP8b (constant) Rat Mouse IgG2a 177515 Anti-RBM17...Mouse IgG2a 177521 Anti-TARPGamma2/Stargazin [N245/1R] TARPGamma2/Stargazin Rat Mouse IgG2a 177522 Anti-TARPGamma2...Mouse IgG2b 206664 Anti-TRIP8b (constant) [N212/17R-2b] TRIP8b (constant) Rat Mouse IgG2b 206665 Anti-SynCAM4...

...serves as a convenient toolkit to assess the proteostasis status in a wide range of experimental systems. LumiLuc...false positives with the original STARR-seq method. Learn more about STARR-seq in our 27 Hot Plasmids from... signal, sensitivity, wide dynamic range, and resistance to photobleaching has made luciferase a common...luminescence. Gaussia luciferase is secreted and more stable, making time course experiments possible, but its...to that of the fluorophore. Conformationally destabilized Firefly luciferase mutants : A set of three ...three sensors that are increasingly structurally destabilized versions of Firefly luciferase which serves ...luciferase. Transcriptional Element Screening Optimized STARR-seq ( S elf- T ranscribing A ctive R egulatory R...

...transduction of the eye and other tissues. As part of our standard viral production , these viral vectors undergo...The following serotypes were developed in Arun Srivastava’s or Shannon Boye’s lab at the University of Florida...derived from AAV2 capsid and developed by Arun Srivastava’s lab. AAV2(trpYF) The AAV2(trpYF) serotype shows...The AAV2(trpYF) serotype was developed by Arun Srivastava’s lab. It is derived from the AAV2 capsid and ...AAV6(dbY-F+T-V) serotype was developed by Arun Srivastava’s lab. It is derived from the AAV6 capsid and ... future publications, please acknowledge Arun Srivastava and cite Petrs-Silva, et al. 2009. High-efficiency... future publications, please acknowledge Arun Srivastava and cite Petrs-Silva, et al. 2011. Novel properties...

...electrophoresis (PAGE) followed by silver staining or SYPRO Ruby staining and the molecular weight and relative...after negative staining. Empty vector particles can be identified after negative staining and appear darker...of Viral Production Viruses are generated using standard methods that have been optimized for each specific... 2–3 fold higher than those achieved using the standard qPCR method. Titering by the University of Pennsylvania...high quality viral vectors by optimizing and standardizing production protocols and performing rigorous...purity of the AAV preparation. Figure 1: Silver staining of purified and non-purified AAV subjected to ...production are propagated in the endA -mutated NEB Stable strain of E. coli . In addition, plasmids are typically...

...vectors with various resistances. Comprehensive multi-cloning site compatible with standard cloning using many...plasmids, see here . pwtCas9-bacteria 44250 CRISPR Stanley Qi Anhydrotetracycline inducible expression of ...plasmids, see here . pgRNA-bacteria 44251 CRISPR Stanley Qi Expression of customizable guide RNA (gRNA) ...epitope tags like His for easy western blotting or staining, and RNA transcript tags to monitor RNA localization...these plasmids you’ll hopefully get a better understanding of what your gene of interest does when expressed...want to determine its structure using X-Ray crystallography, you’ll likely need to purify it. Luckily, ...Requires a strain with T7 RNA polymerase. Amp resistant. More expression vectors for protein purification...

... cytosolic NADH/NAD+ redox state Imaging Cytosolic NADH-NAD(+) Redox State with a Genetically Encoded ...FRET sensors to monitor intracellular Zn(2+) homeostasis. Nat Methods. 2009 Oct;6(10):737-40. Maarten ...and ZapCY2, Golgi or ER targeted Measuring steady-state and dynamic endoplasmic reticulum and Golgi Zn2+...localized zinc sensor ZIBG2 Genetically Encoded, Photostable Indicators to Image Dynamic Zn(2+) Secretion ...sensor of ATP:ADP ratio (PercevalHR) Imaging energy status in live cells with a fluorescent biosensor of the...sensors for cAMP feature exceptional brightness, photostability and dynamic range: characterization of dedicated... sensor with enhanced chloride sensitivity, photostability and reduced ph interference demonstrates augmented...

...shRNA empty plasmid with puro resistance. See plasmid 24150 for hygro resistance. Weinberg 10878 pLKO.1 – ...significant immune response. These viruses also integrate stably into the host genome, enabling long term transgene...Wiederschain 11578 pSico 3rd Conditional (Cre-lox), stable expression of shRNAs; addition of Cre turns on ...fusion; can be used for cDNA expression; puro resistance Sabatini 14883 FUGW 3rd hUbC-driven EGFP; can...expression, gateway plasmid, constitutive Hygromycin resistance gene coexpression. See article for other versions...neomycin selection. See plasmid 21915 for Puro resistance. Wiederschain 36084 pLV-mCherry 3rd CMV driven...

... pHD066 mCherry-Cre fusion and Venus Elastase Zebrafish Stainier 84033 pHD157 mCherry-Cre fusion and Venus...Mombaerts 17408 Puro.Cre empty vector Cre, Puro resistance and miRNA expression PGK Lentiviral Jacks 17736...ins-cre_cryaa-Venus Cre-Venus fusion insulin Zebrafish Stainier 24593 AAV-pgk-Cre codon optimized Cre PGK AAV ... 61573 Rasgrf2-2A-dCre targeting vector DHFR-destabilized Cre; targeting vector for Rasgrf2 Mammalian ...Cre-ERT2 Tamoxifen-inducible Cre None Zebrafish Stankunas 82696 pCRE-iRFP670 Cre and iRFP670 PGK Mammalian... Venus fabp10 Zebrafish Stainier 85040 pK170.AAV-TRE-Cre-WPRE (Supernova) TRE-Cre based Supernova ...Tc-hsp68:Cre) Cre Tribolium casteneum hsp68 T. castaneum (red flour beetle) Averof 119868 pGEM-NLS-Cre-...

...Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering SynBio Depositing...Michel Maharbiz Thorsten Mascher Kathleen Matthews Anastasios Melis Aindrila Mukhopadhyay Richard Murray Erin...Sauro Claudia Schmidt-Dannert Pamela Silver Lei Stanley Qi Jeff Tabor Jan-Willem Veening Christopher Voigt...community. To deposit, view our SynBio Deposit Guide or start our online deposit process . Browse SynBio Plasmids...Genetically Engineered Machine Foundation Registry of Standard Biological Parts - Repository of community-created...WetWare - Open forum for synthetic biologists SEVA - Standard European Vector Architecture Open Plant - Plant...

... Lentivirus Stable RNA <8 Kb 80-130 Dividing/Non-dividing Yes Low AAV Transient or Stable* single stranded...-dividing cells. These viruses enable long-term stable gene expression and are permanently integrated ...viral genomes of choice (Fig. 1B) . Thus, through standard plasmid cloning, viruses can be engineered to ... can be easily modified and amplified (through standard cloning methods). There are different types of... 105 Dividing/Non-dividing No High γ-Retrovirus Stable RNA <8 Kb 80-130 Dividing Yes Moderate * Recombinant...

...system that allows sustained cell loss and ablation of previously resistant cell types. CreLite... fish in the minnow family. It has long been established as a powerful vertebrate model organism for the...high-throughput screening and non-invasive live imaging. A standard toolbox of genetic methods, such as Tol2 transposons...CRISPR/Cas9-based gene editing make the zebrafish a stalwart research model. With more than 70% of human disease...Fusion Construct Set - Amro Hamdoun Lab. A useful “starter kit” for screening fluorescent protein fusion expression...