...the Halo-tagged protein in question, it is targeted to the proteasome for degradation. The HaloTag is ...visualization of proteins of interest. A fluorescent Halo ligand can be added to media, buffer, etc., which...Nabet et al., Chemical Biology, 2018.    HaloTag The HaloTag was initially developed as an affinity tag...straightforward system – HaloTag and its PROTAC are all that is needed to facilitate degradation. HaloTag is also the...proteolysis-targeting chimera (PROTAC) was developed for HaloTag. PROTACs are engineered molecules which target ...involves imaging at some stage, then choosing a HaloTag could pay dividends down the road. It is difficult...

... 204223 CMV-Halo-10-PFN1-Y6D PFN1 Halo, Tev CMV ALS Jessica Henty-Ridilla 204224 CMV-Halo-10-PFN1-R88E...R88E PFN1 Halo, Tev CMV ALS Jessica Henty-Ridilla 204225 CMV-Halo-10-PFN1-G118V PFN1 Halo, Tev CMV ALS Jessica... 161767 FUS_Halo_N_allele_400 FUS Halo ALS Michael Ward 161768 FUS_Halo_C_allele_400 FUS Halo ALS Michael...Melcher 178115 ANG_Halo_C_allele ANG Halo ALS Michael Ward 178116 PRNP_Halo_C_allele PRNP Halo Dementia Michael...178117 PFN1_Halo_C_allele PFN1 Halo ALS Michael Ward 178118 CHCHD10_Halo_N_allele CHCHD10 Halo ALS Michael...178119 UBQLN2_Halo_C_allele UBQLN2 Halo ALS Michael Ward 178120 SIGMAR1_Halo_C_allele SIGMAR1 Halo ALS Michael...178121 APOE_Halo_C_allele APOE Halo Alzheimer's Michael Ward 178122 TREM2_Halo_N_allele TREM2 Halo Alzheimer's...

...with containing chemical tags such as the SNAP-tag, Halo-tag, and CLIP-tag (Kohl et al., 2014) (Addgene #104106...

...fluorescent (EGFP, tagRFP, mCherry) and nonfluorescent (Halo, SNAP) tags, a selectable marker (floxed Cbr-unc...small epitope tags (HA, Myc, Strep tag II, AviTag, HaloTag, SpyTag). The Foerstemann lab developed a polymerase...

...) Labeling scheme. b) COS-7 cell ER labeled with Halo-Sec61β with SiR-CA (magenta) and outer mitochondrial...known as HaloTag is actually HaloTag7, and recent specialized variants run up to HaloTag11. HaloTag is now... (Gallagher et al., 2009). HaloTag: Scientists at Promega developed HaloTag in 2008 (Los et al., 2008)...2008), based on a bacterial haloalkane dehalogenase that binds a synthetic chloralkane (CA) ligand. The current...159 Trimpethoprim  (TMP-tag) Few Easy HaloTag 295 Chloralkane (CA) Many Easy CLIP-tag...proteins (often up to 10-fold more). So, labeling a HaloTag or SNAP-tag construct can help you better detect...protein of interest. For example, the Cohen lab used HaloTag for their “ticker tape” method for intracellular...

...enhancements such as: Human codon optimization Example: Halo Increased photocurrent amplitude Examples: eNpHR...strain Shark), used to silence neuronal activity 632 Halo/NpHR Light-driven inward chloride pump from Natronomonas...plasmids . Halorhodopsins Halorhodopsins are light-gated inward chloride pumps isolated from halobacteria. Wild-type...Jaws Browse Halorhodopsin plasmids . Archaerhodopsins Archaerhodopsin-3 (Arch) from Halorubrum sodomense...variant 517 Halorhodopsins Jaws Red-shifted, light-driven inward chloride pump from Haloarcula (Halobacterium...Wild-type halorhodopsin, known as NpHR (from Natronomonas pharaoni ), causes hyperpolarization (inhibition...Arch Light-activated outward proton pump from Halorubrum sodomense 566 eArch 3.0 Arch with addition of...

...biosensor that utilizes Renilla luciferase and a Halo tag to assay mRNA decay in real time. pKC-4.04, ...wavelengths : NanoLuc® with either SNAP‐tag or HaloTag7 tag in which labeling of the self‐labeling tag...

... (including His, S-tag, OneStrep, CBP, Trx, GST, Halo, MBP, NusA and SUMO) for optimizing expression, ...identified a family of GFP proteins in the cephalochordate Branchiostoma floridae, named bfloGFPs. They...

...pylRS nitroY/haloY-F5 PylRS M. alvus 3-nitrotyrosine (3-nitro-Y), 3-halotyrosine (3-halo-Y) Bacterial ... 160377 pDule-Mb haloTyrRS C6 C6 HaloTyrosine tRNA synthatase M. barkeri Halotyrosine Amino Acids Bacterial...160378 pDule2-Mb haloTyrRS C6 C6 HaloTyrosine tRNA synthatase M. barkeri Halotyrosine Amino Acids Bacterial...barkeri Mammalian Ryan Mehl 141174 pAcBac1-haloTyrRS C6 C6 HaloTyrosine tRNA synthatase E. coli Mammalian Ryan...Kensaku Sakamoto 197099 pIYN3 TyrRS M. jannaschii halogenated tyrosines Bacterial TAG Kensaku Sakamoto 197100...

...bacteriorhodopsin; a comparative study of strains of Halobacterium halobium differing in pigmentation." Biochemical...Rhodopsin-like protein from the purple membrane of Halobacterium halobium." Nature new biology 233.39 (1971): 149...opsin genes, and include bacteriorhodopsins, halorhodopsins, and channelrhodopsins, the first two found... excitation, whereas yellow light activates halorhodopsins to silence neuronal activity (Figure 1) (Wiegert...of the DA neurons produce behavioral and electroencephalography evidences of arousal in mice previously...

...externally-timed bands of color with HaloTag-iPAK4 by applying different HaloTag dyes and showed they could record.... B) HEK cell with an iPAK4 fiber labeled with HaloTag-iPAK4. Scale bar 10 µm. C) Images of iPAK4 fibers...fibers in cultured rat hippocampal neurons. HaloTag dyes are switched from yellow to red at 0 h and back...

...interchangeable labeling using the HaloTag® system, the new Janelia Fluor HaloTag Ligands expand the self-labeling... self-labeling proteins would be SNAP-tag® and HaloTag®. These consist of a genetically encoded enzyme...2017). These dyes were adapted into ligands for HaloTag® and are currently offered at Promega. Due to the...

...SNAP-tag, and HaloTag fusions, while the anti-HA frankenbodies include mEGFP, mCherry, and HaloTag. ...

..., Gautier A. Nat Commun. 2019 Arnaud Gautier HaloTag TagBiFC allows exogenous labeling for single-molecule...and reversible complementation. TagBiFC (split HaloTag) provides maximum flexibility in color labeling...

...earlier Voltron indicators (a fusion of Ace2N and HaloTag, Figure 4), they determined that an A122D mutation...changing the dye that becomes conjugated with its HaloTag domain.  Find Voltron2 plasmids here! Abdelfattah...

..."Optical control of zebrafish behavior with halorhodopsin." Proceedings of the National Academy of Sciences...

...Depositor  Notes pAAV.CAGFLEX.(cyto).iATPSnFR2.S29W.A95K.HaloTag AAV5 Biosensor Tim Brown New viral prep ...

...fluorescent proteins and tags including mCherry, HaloTag and SNAP-tag for multi-color imaging. Find the ...

...guide sgRNA-Sirius-8XMS2 which will recruit the HaloTag with red fluorescent ligands to the loci of interest...

...isolated from Oleispira antarctica, and the P. haloplanktis Trigger Factor), that would allow the stable...