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Showing: 1 - 20 of 151 results
  1. Meet Aavery, Blugene's New Virus Friend!

    Type
    Blog Post
    ...months of input and polling, Blugene's new virus friend finally has a name! We thank you for your participation...December, we introduced you to Blugene’s new virus friend (they/them/theirs). But, they didn’t have a name...
  2. Help Us Name Blugene’s New Virus Friend

    Type
    Blog Post
    ...hashtag #BlugeneVirusFriend on Twitter. As with Blugene’s name, we’d like to give their virus friend a gender...supported us over the past eight years now has a new friend to join them. Blugene would like you to meet......help in finding a good name for Blugene’s virus friend.  During the month of January, we’ll be taking...Have fun! Suggest a name for Blugene's new virus friend here Additional resources on the Addgene blog ...
  3. Science Career Options

    Type
    Blog Post
    ...aspects to determine are working hours (9am-5pm Mon-Fri or overtime and weekends, part time or full time)...
  4. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ...before they had even established their own labs.  Fried chicken with a side of inspiration  I look back ...had subsequently changed fields and my cohort of friends and colleagues had moved on as well, so I knew ...great dinner at Pittypat’s Porch with old and new friends while attending ASM Microbe in Atlanta, GA, in ... time at the #BinningSingletons mixer with new friends @iprophage @robincagle5 pic.twitter.com/52KQDMeSjO...
  5. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Frataxin FXN Friedreich ataxia Ronald Kahn 11443 pBS human Frataxin (myc tag) FXN Myc T7 Friedreich ataxia ...Frataxin FXN Friedreich ataxia Ronald Kahn 11504 pBabe hygro human Frataxin FXN Friedreich ataxia Ronald...AU1 CMV Friedreich ataxia Michael Ristow 14977 pCINeo-hFX(I154F)-HA FXN HA, AU1 CMV Friedreich ataxia ... hFXN Friedreich ataxia Michael Ristow 14979 pGL3-basic-hFX-promotor(1,3kb) FXN hFXN Friedreich ataxia...distal) FXN hFXN Friedreich ataxia Michael Ristow 14987 pBlue-hFX-HA FXN HA, AU1 T7 Friedreich ataxia Michael..., AU1 T7 Friedreich ataxia Michael Ristow 14989 pBlue-hFX(T133A)-HA FXN HA, AU1 T7 Friedreich ataxia Michael... AU1 T7 Friedreich ataxia Michael Ristow 14991 pET28(a)-hFX-HA FXN HA, HIS, AU1 T7 Friedreich ataxia Michael...
  6. AAVED

    Type
    Collection
    ...adoption and scientific outcomes. Event Details Date: Friday September 21, 2018, 9 AM - 5 PM Location: Addgene... and Connect with Fellow Speakers and Attendees Friday, September 21 9:00 AM Breakfast and Welcome Melina...
  7. Luciferase Plasmids

    Type
    Collection
    ...hPGK Lentiviral expression of Venus-Akaluc Roland Friedel 120798 pRRL Luciferase Firefly hPGK Lentiviral ... expression of Venus-firefly luciferase Roland Friedel 170575 pCMV-FLuc Firefly CMV Retroviral expression...
  8. KLF Research Plasmids

    Type
    Collection
    ... George Daley Jin-Tang Dong Steven Dowdy Scott Friedman Konrad Hochedlinger Rudolf Jaenisch Keisuke Kaji...
  9. CRISPR Plasmids - Parasites

    Type
    Collection
    ...malaria), Toxoplasma (toxoplasmosis), Trypanosoma (African sleeping sickness), Cryptosporidium (cryptosporidiosis...
  10. TALEN Engineering

    Type
    Collection
    ...target sites and then generates individual user-friendly graphical roadmaps for each target that guide ...
  11. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TCGGGGACAGAGGCGGCAaacggaacggcccatcAGAACGGCGCTCCGCCCA Frizzled 5 TAL3262 & TAL3263 TCGGATTTTGGCTGCATGtcctgctgctgtttcaACTGTCTGGGCTCGGAGA...
  12. Chemogenetics Guide

    Type
    Guide
    ...Crespo EL, Lambert GG, Prakash M, Ikefuama EC, Friedman N, Brown TM, Lipscombe D, Moore CI, Hochgeschwender...
  13. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    ...wanted to make sure our friend matched our core branding. Blugene needs to be friendly, personable, fun, and...science-inspired friends. As you can see on the homepage, Blugene is now accompanied by an AAV friend.  We encourage...big update on the homepage. Blugene, our trusted friend and mascot, has undergone a make-over as well. ...now has a full expression. You’ll mostly see a friendly smile, but occasionally you might notice Blugene...
  14. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...lines (European, East Asian, Indigenous American, Africa, or South Asian) used in the screens based on the...CRISPR libraries. Samples are divided into those of African ancestry(orange) and those of other ancestry groups...work revealed that cell lines from individuals of African descent have approximately 20% more single nucleotide...found more ancestry-associated dependencies in African cell lines than in any other ancestry group. With...the existing methodology, they estimate that the African ancestry-associated cancer dependencies are undercounted...cancers that are more common in individuals of African descent.Correcting the bias  While their thorough...
  15. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ... some tips and tactics that have worked for my friends, my colleagues and me. To that end, here are my...working and come home happy in the evening, your friends and family will truly sense and appreciate this... more often than not. You want your family and friends to know you care, but they need to know that you...your career and life as well as your family and friends. If there is any guilt, you should be sharing it...easier and to have more time to relax and be with friends and family. #7 Thou shalt create community to help...massage – no guilt. Have recreational time with your friends – no spouses or kids. The bottom line We don’t ...
  16. Our New Antibody Mascot is....

    Type
    Blog Post
    ...we introduced you to Blugene and Aavery's newest friend, our Addgene antibody mascot, here to help us with...Blottie? Which name best fits our new immunoglobulin friend? Please help us decide by voting for your favorite... attention, please, as they introduce their new friend…  Fig. 1: Meet...well, frankly, we're not...not sure yet!   This as-yet–unnamed friend is a huge fan of scavenger hunts. In fact, they’ve been...distributing affinity reagents. Name Our New Antibody Friend We need your help naming our latest mascot, an...
  17. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...some food with friends and come back to the lab to split my cells later. Eating with friends has a somewhat...mammalian cell culture, I would often respond to friends asking me to dinner saying something like, “I’ll...prioritized splitting cells over meeting up with friends, but didn’t realize it until it was too late. I...somewhat immediate deadline - even the best of friends get hangry at some point. My cells, on the other hand...but I lost all the utility (fun) of eating with friends by splitting my cells first. Time management through...
  18. Kit Free RNA Extraction

    Type
    Protocol
    ...10, 2020 Equipment Refrigerated microcentrifuge If you only have a non-refrigerated microcentrifuge, see...RNA degradation. If you don’t have access to a refrigerated centrifuge, you can carefully bring a centrifuge...
Showing: 1 - 20 of 151 results