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  1. Kit Free RNA Extraction

    Type
    Protocol
    ...10, 2020 Equipment Refrigerated microcentrifuge If you only have a non-refrigerated microcentrifuge, see...RNA degradation. If you don’t have access to a refrigerated centrifuge, you can carefully bring a centrifuge...

  2. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...Harvest antibody Equipment Biosafety Cabinet 4 °C Refrigerator Micropipettes and tips Pipettor and pipettes...Example feeding schedule: Thursday: Transfect cells. Friday (24 h post-transfection): Add 4% BCD Feed. Monday...

  3. Water Bath Protocol

    Type
    Protocol
    .... Water baths can also be placed in walk-in refrigerators to achieve a temperature between 4°C and room...

  4. General Transfection

    Type
    Protocol
    ...1x10 6 cells in a T75 flask in a volume of 15 mL. Friday: Plate 8x10 5 cells in a T75 flask in a volume ...

  5. Lentivirus Production

    Type
    Protocol
    ... 6 cells in a T75 flask in 15 mL DMEM Complete. Friday: Plate 8×10 5 cells in a T75 flask in 15 mL DMEM...
Showing: 1 - 7 of 7 results