...co-founder of BGI-China presenting, it’s no wonder it was attended by over 300 Chinese scientists eager...Having never been to China myself, I was pleased to be able to participate when the organizers of the...Dmitry Samarsky, and a very welcoming team from China’s Ribobio, the illustrious speaker list attracted...were potential Addgene requestors and depositors. China is in one of the top 10 countries in number of plasmid...where one can shop for components of traditional Chinese medicine. There were hundreds of stalls selling...replication of RNA in the absence of evolved enzymatic machinery (i.e. RNA replication with no proteins). The chemistry... china...

...successful ChIP experiment! Image courtesy of https://www.wannapik.com/   Even though ChIP requires ...Chromatin immunoprecipitation (ChIP) is an extremely useful technique that provides insight into protein...protein:DNA interactions. ChIP works by using antibodies to capture protein:DNA complexes with antibodies...downstream methods, such as qPCR or next-gen sequencing. ChIP is an incredibly useful application for exploring...an approach for the faint of heart!  The Process ChIP can be done for either tissues or cells. The process...step. For this blog, we're going to focus on the ChIP process and not the upsteam sample collection process...process or the downstream analysis process. And ChIP itself begins at...  Crosslinking Once you have your...

...Fc region may be changed.   Chimeric antibodies can help A chimeric antibody is made by changing an... are subtle, chimeric antibodies should be revalidated before use. In addition, chimeric antibodies will... a similar problem, then isotype converted, or chimeric, antibodies may be the right solution for you.... B antibody to a mouse IgG2a Fc. The resulting chimeric antibody would have the original variable regions...isotype (Liddell 2013). In most cases, the resulting chimeric antibody retains the binding specificity to the...utility of recombinant antibody tools, the resulting chimera may have different binding properties (Morelock...isotype-specific secondary antibodies when multiplexing with chimeric antibodies. In the case above you should use an...

...on to work in patent law, business development, teaching, etc. Church also encourages his mentees to follow...

...plasmids from this publication at Addgene. 3. Zhang, Chi, Xiuhua Meng, Xiaolei Wei, and Ling Lu. (2016). “...

...knockout in immune systems.   CHIME We previously developed CHIME (CHimeric IMmune Editing system) to enable...have not been widely employed in vivo. The CHIME and new X-CHIME systems, developed and deposited at Addgene...hindering studies post embryogenesis. Using C-CHIME and S-CHIME, we discovered that double knockout of PTPN1...Schematic showing the X-CHIME approach. Figure from LaFleur, et al. 2024.   The X-CHIME deposit also includes...preparations than the first generation CHIME (pXPR_053). Find X-CHIME plasmids here!   Marty LaFleur is a...LaFleur et al., 2019a and LaFleur et al., 2019b). CHIME works through transduction of Cas9-expressing (Rosa26...quite useful for interrogating these scenarios. X-CHIME We recently developed and deposited four new systems...

...unrelated worm (C. elegans) [2]. Delivering dsRNA matching a specific mRNA sequence triggers a cascade of... through physical blocking of the translation machinery. In the Sánchez lab protocol, dsRNAs are delivered...

... a scientist, it is useful to make literature searching and reading a regular part of your routine. Set...scientific literature a little bit at a time. Searching for literature My go-to search engines for finding...a network with an institutional subscription), archived on PubMed Central, or in preprint onBioRXIV. One...Mendeley – these programs offer more options for searching and sorting your own digital library as well as...some integration with internet-wide literature searching. Update 5/6/2015:Here's another option that may... of their F1000Workspace.  Give your favorite searching, reading and organization tips in the comments...

...and have coaching plans that are financially accessible. Sometimes only a few hours of coaching time can...Some have online coaching tools that can be very effective and also help keep the coaching costs low. Most...also launched an online career coaching program that might make her coaching more affordable for you. There...making this transition). Another coaching group is Still Point Coaching. One nice thing about a group is...Professional Career Coaching can be an excellent tactic for scientists making a career shift or who wish...exception, that this helps people do a better job at reaching their goals.  Check out Joanne's Reddit AMA I...that few scientists are aware of the benefits of coaching relationships and do not know where to look for...

...engineered DNA-binding molecule-mediated ChIP (enChIP). By using enChIP, specific genomic regions can be easily...technologies. The locus-specific ChIP technologies consisting of iChIP and enChIP are recent examples. We also...locus-specific ChIP). These methods consist of insertional chromatin immunoprecipitation (iChIP) and engineered...the insertional chromatin immunoprecipitation (ChIP) (iChIP) technology, which uses locus-tagging and affinity...requested plasmids for enChIP through Addgene. Retroviral Expression System for enChIP using CRISPR Addgene...molecule-mediated chromatin immunoprecipitation (enChIP), both developed in the lab. In June 2014, Dr. ...  Addgene: Your lab has worked extensively with enChIP systems. Can you describe this technology and its...

...the interview with Joachim Goedhart Podcast breakdown Intro Chapter 1 - How Joachim ended up at the University...art. Outro Many thanks to our guest, Joachim Goεdhart! Joachim Goedhart is an assistant professor at...Scientist Benoit Giquel. Ben recently spoke with Joachim Goεdhart, a professor at the University of Amsterdam...University of Amsterdam Chapter 2 - Joachim’s Academic and career goals. Career advice for people going... microscopy. Chpater 4 - Research projects in Joachim’s lab. New microscopy probes and more. Chapter 5...fluorescent probes. You can follow him on twitter: @joachimgoedhart.   Special thanks to our sound engineer/producer...Fluorescent Proteins featured topic page Read Joachim's Series on Choosing the B(right)est Fluorescent...

... our paper, “Synthetic recombinase-based state machines in living cells” (1), into a more straight-forward...our engineered devices, recombinase-based state machines for your own experimental needs. For more context...the paper. Why might one be interested in state machine technology? Biological research has produced a ...timing of these factors. Recombinase-based state machines (RSMs), which take on a particular DNA-sequence... set of possible downstream states that can be achieved by expression of other recombinases. Multiple ...terminators, etc) are composed. Fear not – the state machine design space is highly degenerate and often times...Roquet et al., Synthetic recombinase-based state machines in living cells. Science. 353, aad8559 (2016)....

...naïve questions. During the panel, an artist was sketching her interpretation of the discussion in markers...

...gene. CHIME can also be used for high throughput screens using pooled libraries. Find the CHIME plasmids...successfully visualized in vivo using ultrasound in Escherichia coli and Salmonella Typhimurium. This work demonstrates...cell lineages in vivo using the CRISPR-Cas9 system CHIME Article contributed by Aliyah Weinstein  Listen ...the Harvard Medical School developed a solution: CHIME, a CRISPR-Cas9 system that can edit genes in any...

... (2002): 682-688. PubMed PMID:12404098.  4. Lin, Chi-Wang, Cindy Y. Jao, and Alice Y. Ting. "Genetically... which genes should be ‘read’ by the cellular machinery. This is how most cells in the body contain the...compacted it becomes. This prevents the cellular machinery from physically interacting with the genes in ...to have occurred that was passed down to the grandchildren; in this study,epigenetic changes were statistically...influenced cardiovascular mortality of the female grandchildren." BMC genetics15.1 (2014): 12. PubMed PMID: ...

...219444 Anti-KChIP1 [K55/9R] KChIP1 Rat Mouse IgG2a 219445 Anti-KChIP1 K+ channel [K55/59R] KChIP1 K+ channel...190284 Anti-KChIP3 K+ channel [K66/27] KChIP3 K+ channel Rat Mouse IgG2a 190285 Anti-KChIP3 K+ channel ... 206760 KChIP3 K+ channel scFv [K66/27] K66/27 scFv KChIP3 K+ channel Rat Mouse 206761 KChIP3 K+ channel...38 scFv KChIP3 K+ channel Rat Mouse 206762 KChIP3 K+ channel scFv [K66/59] K66/59 scFv KChIP3 K+ channel...Human Mouse IgG2a 177442 Anti-Pan-KChIP K+ channel [K55/82R] Pan-KChIP K+ channel Mouse IgG2a 177443 Anti-CASK...CASK Human Mouse IgG2a 177444 Anti-KChIP2b K+ channel [K60/73R] KChIP2b K+ channel Rat Mouse IgG2a 177445...external) Human Mouse IgG2a 188175 Anti-KChIP2b K+ channel [K60/87R] KChIP2b K+ channel Rat Mouse IgG2a 188176...

...Locus-specific ChIP technologies consist of insertional chromatin immunoprecipitation (iChIP) and engineered...chromatin immunoprecipitation (enChIP; Fujita & Fujii, 2013). In iChIP, specific genomic regions tagged... Fujii Lab web page . Figure 1: iChIP general scheme Figure 2: enChIP general scheme Fujii Lab Plasmids...3xFLAG-dCas9/pCMV-7.1 enChIP plasmid, expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify...8xLexA-binding elements/pMD20 iChIP plasmid, LexA-binding elements 48874 3xFNLDD/pCMV-7.1 iChIP plasmid, expresses...3xFNLDD/pMXs-I2 iChIP plasmid, expresses 3xFNLDD in mammalian cells 48972 3xFNLDD/pMXs-IG iChIP plasmid, expresses...protein for enChIP analysis. Fujita T, Kitaura F, Oji A, Tanigawa N, Yuno M, Ikawa M, Taniuchi I, Fujii ...

...Elsaesser 140020 pAS_4xMma PylT_FLAG-AbKRS-chIPYE chimeric PylRS Chimeric photo-crosslinking-lysine Mammalian...translation machinery. To expand the genetic code, 4 major changes to the standard translation machinery are ...Bacterial Jason W Chin 174078 pDule-3-nitroTyrosine (A7) 3NY (A7) synthetase M. jannaschii 3-nitroTyrosine...Acd-A9 M. jannaschii acridone Bacterial TAG Ryan Mehl 197652 pDule2 - Mj Acd A9 Acd-A9 M. jannaschii acridone... sets. Many of these sets are derived from M. jannaschii , M. barkeri , or E.coli and can be mutated ...PI 31186 pEVOL-pAzF p-azidohenylalanine RS M. jannaschii p-azido-l-phenylalanine Bacterial TAG Peter Schultz...31190 pEVOL-pBpF p-benzoylphenylalanine RS M. jannaschii p-benzoyl-l-phenylalanine Bacterial TAG Peter...

...iCre CAG Mammalian Capecchi 113849 pCAGGS-pac-T2A-iCre iCre CAG Mammalian Capecchi 116879 CAG-Cremyc-2A-GFP...Mammalian Capecchi 125749 pCAG-sfGFP-iCre-ERT2 iCre-ERT2 - Tamoxifen inducible CAG Mammalian Capecchi 125821...Mammalian Capecchi 125822 pCAGGS-mTagBFP2-T2A-iCre (v2) TagBFP, iCre CAG Mammalian Capecchi 126006 pAAV2...Mammalian Cepko 69916 pAAV.cTNT.iCre iCre and tdtomato Chicken cardiac troponin T AAV Pu 70120 pEMS2159 iCre with...unskippable linker) - Tamoxifen inducible CAG Mammalian Capecchi 128167 pCAGGS-mTagBFP2-T2A-sfGFP-GSAx9-iCre-ERT2...GSAx9 linker) - Tamoxifen inducible CAG Mammalian Capecchi 128168 pCAGGS-mTagBFP2-T2A-iCre-ERT2 iCre-ERT2...-terminus) - Tamoxifen inducible CAG Mammalian Capecchi 128171 pCAGGS-mTagBFP2-T2A-iCreERT2 (PV) iCre-...