...transcription to begin. In addition to arabinose, cAMP complexed with cAMP activator protein (CAP) can also stimulate...Supplementing cell growth media with glucose decreases cAMP and represses pBad, decreasing promoter leakiness...

... pii: 11/520/eaah3738. Justin Blau cAMP (cyclic AMP) cAMPFIRE cAMP biosensors with improved sensitivity...Commun. 2013;4:2550. Gary Yellen cAMP (cyclic AMP) Yellow fluorescent cAMP indicator (Flamindo2) Genetically-encoded...e100252. Tetsuya Kitaguchi cAMP (cyclic AMP) Red fluorescent protein-based cAMP indicator (Pink Flamindo...subcellular imaging of cAMP in vivo. Nat Methods. 2022 Nov;19(11):1461-1471. Haining Zhong cAMP (cyclic AMP) RAB-ICUE...-0200-6. Jin Zhang cAMP (cyclic AMP) Yellow-emitting homoFRET/anisotropy ICUE cAMP sensor Single-color...-8674(20)31399-4. Edward Boyden cAMP (cyclic AMP) FRET sensors of cAMP levels for imaging in live cells...e0122513. Kees Jalink cAMP (cyclic AMP) Fluorescent protein exchange sensor for cAMP Ratiometric biosensors...

...in 2010 for visualizing the transcription factor cAMP-responsive element-binding protein (CREB) in living...were then refined by a secondary screen in rat hippocampal neuron cultures. The optimized Twitch sensors...

...previous publications. They also developed calcium, cAMP, and ATP sensors based on Nano-lantern, showing ...

...Decrease cAMP Neuronal inhibition Armbruster et al., 2007 KORD Human κ-opioid G αi SALB Decrease cAMP Neuronal...signaling by inhibiting adenylate cyclase and downstream cAMP production. There are currently two Gi DREADDs based...activate neuronal signaling by increasing intracellular cAMP concentrations. There is currently one Gs-DREADD...muscarinic & turkey β1-adrenergic G αs CNO* Increase cAMP Neuronal burst firing Guettier et al., 2009 hM4Di.... A Gαs DREADD Mouse for Selective Modulation of cAMP Production in Striatopallidal Neurons. Neuropsychopharmacol...

...pathways HDR mechanisms can be divided into two main camps: non-conservative and conservative. The conservative...

.... "Fourth-generation epac-based FRET sensors for cAMP feature exceptional brightness, photostability and...

...adenylyl cyclase from Beggiatoa sp. for light-induced cAMP modulation. Utilizes BLUF (blue light utilizing ...

...There are three GCaMP types based on their kinetics: GCaMPf (fast) GCaMPm (medium), GCaMPs (sensitive but...zebrafish and mammalian systems. The GCaMP6, jGCaMP7, and jGCaMP8 sensors are also suitable for in vivo...slower). GCaMPf may be suitable for very rapid measurements, like action potentials in neurons. jGCaMP7 and...localize at the plasma membrane. GCaMP-X is a nucleus-targeted GCaMP6 with an extra apoCaM-binding motif...axon (GCaMP6 only). The genetic elements required for Drosophila studies have been added for jGCaMP7 and...to have slower, but larger, calcium changes. The GCaMP6 family of sensors are commonly used calcium reporters...and jGCaMP8 are also available with improved kinetic and sensitivity parameters. Sensitivity and Localization...

...requests faster than jGCaMP7 (Figure 5B).   Figure 5: A) GCaMP6, jGCaMP7, and jGCaMP8 viral preps distributed...Figure 5A). We then added jGCaMP7 and eventually jGCaMP8, later generations of GCaMP6, to the service. While...) Inset showing distribution of only GCaMP6, jGCaMP7, and jGCaMP8 per year from 2016–2023. All three...generations: jGCaMP8 allows for the “picture” of a train of action potentials that GCaMP6 and jGCaMP7 do not... for instance, the GCaMP-encoding viral vectors available in the repository. GCaMP, a calcium indicator...they were both requested, GCaMP6 remains the more requested option, and jGCaMP8 appears to be growing in...be a matter of timing, as jGCaMP7 was released relatively soon after GCaMP6. It could be a matter of the...

...-fold brighter than GCaMP6s. jGCaMP7f is the fastest responding variant. jGCaMP7b exhibits the brightest...pGP-AAV-syn-jGCaMP7f-WPRE (104488-AAV8) pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (104492-AAV8) pGP-AAV-syn-jGCaMP7c variant...potential, GCaMP7s is approximately twice as bright, relative to baseline fluorescence, as the GCaMP7f variant...potentials. This is about twice as bright as GCaMP7f and GCaMP7s. See all our biosensors inventory, and check...Calcium Sensors pGP-AAV-syn-jGCaMP7b-WPRE (104489-AAVrg) pGP-AAV-syn-FLEX-jGCaMP7b-WPRE (104493-AAVrg) pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE...pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE (104495-AAVrg) pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE (104496-AAVrg) Optogenetics...sensors The latest calcium sensors from Janelia (jGCaMP7) continue to be useful for monitoring neuronal ...

...pGP-AAV-syn-FLEX-jGCaMP7b-WPRE) exhibits the brightest resting fluorescence of the GCaMP7 family of calcium...inventory! Our new AAVs include: pGP-AAV-syn-FLEX-jGCaMP7b-WPRE pAAV.hSyn-FLEX.iGABASnFR pAAV.hSyn-FLEX.iGABASnFR.F102G...calcium binding. There are a few versions of these jGCaMP7 sensors with different properties (for example,...applications. We recently released a new variant of jGCaMP7 (keep reading) and will be releasing more variants...like dendrites and axons. Find the 7b variant of jGCaMP7 in AAV1 at Addgene! See all calcium sensor AAV ...Serotype Name 104496  AAV1  GP-AAV-CAG-FLEX-jGCaMP7f-WPRE AAV1 with a CAG promoter exhibits high specificity...Purkinje neurons. 105321  AAV1  pGP-AAV-syn-jGCaMP7c variant 1513-WPRE 7c exhibits high contrast between...

...Flp-dependent GCaMP, AAV8-105714. Several new options are also available for the calcium sensor jGCaMP7 from Janelia... Janelia, including three jGCaMP7 plasmids now packaged in AAVrg. jGCaMP7s, the variant with the most ...104489 AAVrg pGP-AAV-syn-jGCaMP7b-WPRE 104493 AAVrg pGP-AAV-syn-FLEX-jGCaMP7b-WPRE 104496 AAVrg pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE...pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE 104495 AAVrg GP-AAV-CAG-FLEX-jGCaMP7s-WPRE 105714 AAV8 pAAV-Ef1a-fDIO-GCaMP6s...pAAV-Ef1a-fDIO-GCaMP6s Neurotransmitter sensors: Dopamine and norepinephrine Neuroscience research relies on accurate...

...axon-GCaMP6s-P2A-mKate2 (AAV9) pAAV-hSynapsin1-GCaMP6s-P2A-mKate2 (AAV9) pAAV-hSynapsin1-GCaMP6s-P2A-mRuby3...AAV9) pAAV-CAG-SomaGCaMP6f2 (AAV9) Plus, additional AAV serotypes of the most recent jGCaMP8 calcium sensors...sensors: pAAV-hSynapsin1-axon-jGCaMP8s-P2A-mRuby3 (AAV1) pGP-AAV-syn-jGCaMP8f-WPRE (AAV Retrograde) pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE...-AAV-CAG-FLEX-jGCaMP8m-WPRE (AAV Retrograde) pGP-AAV-syn-FLEX-jGCaMP8m-WPRE (AAV5)   There are hundreds...dLight1.2 and voltage sensor Ace2N and additional GCaMP6 constructs: pAAV-CAG-dLight1.2 (AAV1) pGP-AAV-...

...  AAV1 pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE 105322  AAV1 pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE 111262...AAV1 pAAV-hSynapsin1-axon-GCaMP6s 112005  AAV9 pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3) 112010  AAV9... pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE 105714  AAV8  pAAV-Ef1a-fDIO-GCaMP6s Optogenetics ...AAVs include: Control AAVs for serotype testing jGCaMP7c for calcium detection PSAM4 GlyR for chemogenetic...Among other new biosensor tools, we now offer the jGCaMP7c variant in AAV1. This variant exhibits high contrast...AAV9 pAAV-hSynapsin1-FLEx-axon-GCaMP6s New voltage sensors Plasmid Serotype Name 119036  AAV1  ...

...repository, you'll find the latest iteration of GCaMP, GCaMP8, available in new serotypes, as well as seven...Notes AAV-CAG-jGCaMP8s-WPRE AAV1 Biosensor Looger New serotype AAV-CamKIIa-jGCaMP8m-WPRE AAV5 Biosensor...pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE AAV PHP.eB Biosensor Kim New serotype AAV-mDlx-jGCaMP8s-WPRE  AAV9 Biosensor...serotype AAV-hSyn-Soma-jGCaMP8s AAV9 Biosensor Fyhn New serotype AAV-hSyn-Soma-jGCaMP8f AAV9 Biosensor Fyhn...

... Axon targeting GCaMP6f from Lin Tian’s lab is available in serotype AAV1. The GCaMP8 viral vectors from...expressing GCaMP6f with this promoter. Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f are ... have arrived! We now offer several versions of GCaMP8 vectors in serotypes AAV1 and AAV5. AAV9 preps ...viral prep in serotype AAV1. Synapse targeting GCaMP6s from the Rylan Larsen lab are now available in ...ready-to-use AAV5 viral vector. Synaptophysin-tagged GCaMP6s is enriched in the pre-synaptic terminal providing...

...logistical stress that accompanies traveling for campus visits. This is definitely an advantage of surviving...everything you need to during the interview. (Virtual) Campus Visits After receiving applications, universities...students to continue the application process through a campus visit, a weekend where applications can get to ...allow you to get important information about the campus even if you can’t visit. By talking with people...aspects that are confusing? During a traditional campus visit, many students will have questions about ... be your perfect fit! Even if you had planned a campus visit and the in-person portion got canceled, taking...And even if you intend to attend an interview or campus visit in person, things like sickness or scheduling...

...regions. For example, communication between the hippocampus and the prefrontal cortex brain regions is involved...specific example of episodic memory, a subset of hippocampal neurons projects axons directly to the prefrontal...Cre recombinase only in pyramidal cells of the hippocampal region CA2 allowed for detailed mapping of the...approach was used by researchers from the Janelia Campus for their MouseLight project but neurons were traced...University of California, Berkeley, the Janelia Research Campus also developed a new AAV variant, rAAV2-retro, ...PMC2293646. Jin, J., and Maren, S. (2015). Prefrontal-Hippocampal Interactions in Memory and Emotion. Front Syst...type-specific genetic and optogenetic tools reveal novel hippocampal CA2 circuits. Nat Neurosci 17(2), 269–279. PubMed...

...protein-based calcium sensors (jYCaMP1), variants of the calcium indicator jGCaMP7, that are optimized for excitation... sensors include syn-jYCaMP1s (slow variant), the Cre-dependent syn-FLEX-jYCaMP1s, and the axon-targeted...axon-targeted syn-FLEX-axon-jYCaMP1s. Find these AAVs at Addgene Cre-dependent EYFP AAV in the new serotype ...mDlx-GFP, hDlx-FLEX-GFP, hDlx-Flex-dTomato, mDlx-GCaMP6f, hDlx-GiDREADD-dTomato and mDlx-ChR2-mCherry. ...

..., Vibrio cholerae, Xanthomonas campestris pv. campestris, X. campestris pv. malvacearum, Zymomonas mobilis...Shewanella spp., Thiobacillus spp., Xanthomonas campestris IncQ RSF1010, R300B, R1162 Gram-negative...salmonicida, Yersinia enterocolitica, Xanthomonas campestris, X. maltophilia IncW pSa, pR388 Gram-...Salmonella typhimurium, Vibrio cholerae, Xanthomonas campestris   In addition to incorporating broad host...

...which is limited to the hippocampus. Thus, only neurons signaling from the hippocampus to Broca’s area would...poor scalability.  Scientists at Janelia Research Campus and the University of California, Berkeley engineered...access all the neurons that signaled from the hippocampus to Broca’s area. This could be done by injecting...of a mouse specifically expressing Cre in the hippocampus (Fig. 3). The Cre-dependent AAV would access ...

...technician in Kaspar Podgorski’s lab at Janelia Research Campus, HHMI where he works on developing and optimizing... anchoring domain), in electroporated in rat hippocampal cultured neurons, imaged 14 days post-transfection...completed, Hubbard will image these neurons using GCaMP7, measuring calcium signaling related fluorescence... the screen to understand mouse cortical and hippocampal neural microcircuits. “In the longer term, this...

...need. For example, as shown in this study [3], hippocampal neurons are activated when the mouse is placed...expression, thus showing successful inhibition of hippocampal neurons. Initial monitoring Second, this may ...associated with a visual or auditory cue. The hippocampus has long been known to be responsible for mediating...the function of specific subregions within the hippocampus were only recently studied in detail thanks to...

...culminated in the current President holding and campaigning on many views that don't line up with the scientific...at the University of Colorado, Anschutz Medical Campus.  He studies viruses including HIV and is interested...And as we rallied on the University of Michigan campus speakers thoughtfully reminded us to use the same... at the University of Colorado Anschutz Medical Campus in Aurora, CO where she studies the development...

...Biosensor Robert Campbell New viral prep with multiple serotypes AAV-hSyn-Ribo-jGCaMP8s AAV1 Biosensor...Biosensor Marianne Fyhn New viral prep AAV-hSyn-Ribo-jGCaMP8m AAV1 Biosensor Marianne Fyhn New viral prep ...

...strange to be using a micropipette and gloves at a campsite. And it was exciting – one night while trying ...picnic table before dark, a bison wandered into our campsite. We had to abandon our samples and move farther... Figure 3: When a bison wanders into the campsite, leave your samples behind! Paul and Anastasia...

...Monkeys and rodents. Image and caption adapted from Campos et al., 2023. AAV.CAP-Mac is a systemic capsid...730825. https://doi.org/10.3389/fimmu.2021.730825 Campos LJ, Arokiaraj CM, Chuapoco MR, Chen X, Goeden N...Wang, T., Wolfe, D. A., Arokiaraj, C. M., Ngo, V., Campos, L. J., Griffiths, J. A., Ichiki, T., Mazmanian...Ravindra Kumar, S., Arokiaraj, C. M., Plattner, V. M., Campos, L. J., Mich, J. K., Monet, D., Ngo, V., Ding, ...Scherrer, J., Winchester, J., Blackburn, R. J., Campos, L. J., Man, K. N. M., Sun, J., Chen, X., Lefevre...

...studies have found reduced BDNF levels within the hippocampus and frontal cortex during depression, yet there...injecting lentiviral vectors expressing BDNF in the hippocampus causes a shift towards antidepressant behavior...-mediated suppression of microRNA124a in the hippocampus evokes antidepressants-like effects in rats."...

...Neisseria meningitidis (Nm or Nme) NNNNGATT Campylobacter jejuni (Cj) NNNNRYAC Streptococcus thermophilus...).  At 984 amino acids in length, Cas9 from Campylobacter jejuni (CjCas9) is even smaller than SaCas9 ...editing with a small Cas9 orthologue derived from Campylobacter jejuni. Nat Commun 8: . https://doi.org/10.1038...

... we’re here to help with our Suggest a Plasmid Campaign. How to suggest a plasmid The scientists at Addgene...reach out to will end up depositing, we hope this campaign will expand the repository in ways that will be...

...optogenetics, and other imaging tools. The GCaMP5 and GCaMP6 series of genetically encoded calcium indicators...

... sparse (e) labeling of the calcium indicator, GCaMP6f, in the Drosophila optic lobe. Image from Isaacman-Beck...of T5 neurons by placing the calcium indicator GCaMP6f in the effector position, as well as to optogenetically...

...Adapted from Fig. 2 Campbell et al, Nature Methods   Find YFP plasmids here!   Campbell, B.C., et al. Chemically...

... at the University of Colorado Anschutz Medical Campus in Aurora, Colorado, USA. The primary focus of ... at the University of Colorado Anschutz Medical Campus in Aurora, Colorado, USA.     Richard Gronostajski...

...calcium transients in PV interneurons with AAV-S5E2-GCaMP6f, now available as a ready-to-use viral prep in ...cytoplasmic-tdTomato and Synaptophysin-GFP. Synapse targeting GCaMP6s from the Rylan Larsen lab, now available in a ready...

...Calcium Sensors GCaMP8 axon-GCaMP8 soma-GCaMP8 ribo-GCaMP8 GCaMP7 GCaMP6 axon-GCaMP6 soma-GCaMP6 Twitch2B Yellow...Yellow Calcium Sensors jYCaMP1 axon-jYCaMP1 Red Calcium Sensors CaMPARI jRCaMP1 jRGECO1 sRGECO Far-Red..., 2, 9 Campbell Calcium Sensor: HaloCaMP1a 138327 pAAV-synapsin-HaloCaMP1a-EGFP Syn HaloCaMP1a EGFP Constitutive...Constitutive 9 Li Calcium Sensor: GCaMP8f 162376 pGP-AAV-syn-jGCaMP8f-WPRE Syn jGCaMP8f none Constitutive 1, 9, ...pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE CAG jGCaMP8f none Cre dependent 1, 5, 9, rg* GENIE 169258 AAV-hSyn-Soma-jGCaMP8f Syn...soma-jGCaMP8f none Constitutive 1, 9, rg* Fyhn 176750 AAV-CamKIIa-jGCaMP8f-WPRE CamKIIa jGCaMP8f none ...AAV-mDlx-jGCaMP8f-WPRE Dlx jGCaMP8f none Constitutive 1, 9, rg* Looger 176756 AAV-EF1a-jGCaMP8f-WPRE EF1a...

... disease for which there is currently no cure (Campbell et al., 2018). Find plasmids from the Fujii lab...see our interview with Dr. Fujii. References Campbell, Amy E., et al. "NuRD and CAF-1-mediated silencing...

...biosensor, GCaMP, and click through to the plasmid search results: More than 200 different GCaMP containing...

...Luke Lavis, a Group Leader at the Janelia Research Campus, Howard Hughes Medical Institute. Chemistry is ...currently a Group Leader at the Janelia Research Campus, Howard Hughes Medical Institute. He would rather...

...and Postdoctoral Programs at the Janelia Research Campus of the Howard Hughes Medical Institute. Are you...and Postdoctoral Programs at the Janelia Research Campus of the Howard Hughes Medical Institute. His interests...

...brain. The Allen Institute and the Janelia Research Campus in the USA have used AAV1 and AAV2 serotypes in...visualize “thorny excrescence” (TE) spines in the Hippocampal CA3 pyramidal neurons which are known to be notoriously...

...AAV. At 984 amino acids in length, Cas9 from Campylobacter jejuni (CjCas9) is the smallest Cas9 ortholog...editing with a small Cas9 orthologue derived from Campylobacter jejuni. Nat Commun 8: . https://doi.org/10.1038...

... editing enzyme (“Editase”), ADAR2, into the hippocampus of a Rett Syndrome mouse model using an adeno-associated...RNA in each of three neuronal subtypes of the hippocampus. This technology has formed the basis of the ...

...Dre Calcium Sensors GCaMP8f GCaMP8s GCaMP8m GCaMP7f GCaMP7s GCaMP7b GCaMP6f GCaMP6s GECO Other Biosensors...pGP-AAV-syn-jGCaMP7s-WPRE Syn GCaMP7s Calcium sensor Kim 104489 pGP-AAV-syn-jGCaMP7b-WPRE Syn GCaMP7b Calcium...-syn-jGCaMP8s-WPRE Syn GCaMP8s Calcium sensor GENIE 162375 pGP-AAV-syn-jGCaMP8m-WPRE Syn GCaMP8m Calcium...AAV-hSyn-Soma-jGCaMP8m Syn Soma-GCaMP8m Calcium sensor Fyhn 169258 AAV-hSyn-Soma-jGCaMP8f Syn Soma-GCaMP8f Calcium...-mDlx-jGCaMP8s-WPRE Dlx GCaMP8s Calcium sensor Looger 176756 AAV-EF1a-jGCaMP8f-WPRE Ef1a GCaMP8f Calcium...AAV-EF1a-jGCaMP8m-WPRE EF1a GCaMP8m Calcium sensor Looger 176758 AAV-EF1a-jGCaMP8s-WPRE EF1a GCaMP8s Calcium...AAV-CAG-jGCaMP8f-WPRE EF1a GCaMP8f Calcium sensor Looger 179255 AAV-CAG-jGCaMP8m-WPRE CAG GCaMP8m Calcium...

...pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 Biosensor Eric Schreiter AV-1-PV4704 101064-AAV1 pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40...gfaABC1D-lck-GCaMP6f Biosensor Baljit Khakh AV-5-PV3435 100834-AAV5 pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 Biosensor...AV-1-50942 50942-AAV1 pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener...AV-1-50943 50943-AAV1 pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener...-68717 68717-AAV1 pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener...-68719 68719-AAV1 pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener...68720 68720-AAV1 pAAV-hSyn1-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener...

...158757-AAV8 pAAV-CAG-SomaGCaMP6f2 Cell body-targeted GCaMP6f, under CAG promoter: GCaMP6f followed by a linker...AAV9 pAAV-CAG-SomaGCaMP6f2 Cell body-targeted GCaMP6f, under CAG promoter: GCaMP6f followed by a linker...element Gordon Fishell 83899-AAV1 pAAV-mDlx-GCaMP6f-Fishell-2 GCaMP6f expression in forebrain GABA-ergic interneurons...element Gordon Fishell 83899-AAV9 pAAV-mDlx-GCaMP6f-Fishell-2 GCaMP6f expression in forebrain GABA-ergic interneurons... Gordon Fishell 83899-AAVrg pAAV-mDlx-GCaMP6f-Fishell-2 GCaMP6f expression in forebrain GABA-ergic interneurons...111261-AAV9 pAAV-hSynapsin1-axon-GCaMP6m For enriched expression of GCaMP6m in axons Lin Tian 111262-AAV1...AAV1 pAAV-hSynapsin1-axon-GCaMP6s For enriched expression of GCaMP6s in axons Lin Tian 111568-AAV5 pZac2.1...

... β1 (white) and anti-GABA-AR β3 (green). (C) Hippocampus labeled with anti-Kv2.1 (magenta), anti-AnkyrinG...that your target protein is expressed in the hippocampus, or counting the number of puncta in each cell...

...and Postdoctoral Programs at theJanelia Research Campus of the Howard Hughes Medical Institute. Today's...and Postdoctoral Programs at the Janelia Research Campus of the Howard Hughes Medical Institute. His interests...

...Serotype Striatum AAV5>AAV9>AAV8>AAV1>AAV6>AAV2 Hippocampus AAV9>AAV5>AAV8>AAV1>AAV6>AAV2 Auditory Cortex...upon which section you’re looking at (striatum, hippocampus and cortex) within the mouse brain (4). An example...