We narrowed to 1,836 results for: nicola

1. pUAP1

   Plasmid

   #63674

   Purpose

   Accepts DNA sequences via BpiI cloning sites, resulting in Level 0 standard parts for Golden Gate cloning. Parts can be released with a user-defined 4-base-pair, 5 prime overhangs using BsaI.

   Depositor

   Nicola Patron

   Article

   Patron et al New Phytol. 2015 Jul 14. doi: 10.1111/np

   Insert

   RFP cloning selection cassette
   

   Use

   Synthetic Biology

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pICSL90003

   Plasmid

   #68262

   Purpose

   Level 0 Golden Gate Part: Promoter U6 (Triticum aestivum)

   Depositor

   Nicola Patron

   Article

   Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

   Insert

   Promoter: U6 (Triticum aestivum)
   

   Use

   Synthetic Biology

   Available Since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEPQD1CB0112

   Plasmid

   #177044

   Purpose

   MoClo Level 1, position 3, transcriptional unit for transient expression of geraniol synthase (CrGES) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   geraniol synthase (CrGES) from Catharanthus roseus
   

   Use

   Synthetic Biology

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEPQD0CM0818

   Plasmid

   #177020

   Purpose

   Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   geranyl pyrophosphate synthase; geranyl diphosphate synthase
   

   Use

   Synthetic Biology

   Mutation

   transit peptide removed

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEPCTαKN0005

   Plasmid

   #187569

   Purpose

   Transcriptional unit for constitutive expression of nanoluc luciferase driven by the 35s promoter

   Depositor

   Nicola Patron

   Article

   Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

   Insert

   Luciferase
   

   Expression

   Plant

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCk3

   Plasmid

   #136697

   Purpose

   Acceptor plasmid for odd level position 3, lacZ blue-white screening. Part of the OpenPlant toolkit.

   Depositor

   Nicola Patron

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   lacZ
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCk2

   Plasmid

   #136696

   Purpose

   Acceptor plasmid for odd level position 2, lacZ blue-white screening. Part of the OpenPlant toolkit.

   Depositor

   Nicola Patron

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   lacZ
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCk1

   Plasmid

   #136695

   Purpose

   Acceptor plasmid for odd level position 1, lacZ blue-white screening. Part of the OpenPlant toolkit.

   Depositor

   Nicola Patron

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   lacZ
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCk4

   Plasmid

   #136698

   Purpose

   Acceptor plasmid for odd level position 4, lacZ blue-white screening. Part of the OpenPlant toolkit.

   Depositor

   Nicola Patron

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   lacZ
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pICSL90002

    Plasmid

    #68261

    Purpose

    Level 0 Golden Gate Part: Promoter U6-26 (Arabidopsis thaliana)

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter: U6-26 (Arabidopsis thaliana)
    

    Use

    Synthetic Biology

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pICSL11059

    Plasmid

    #68263

    Purpose

    Level 1 Golden Gate Cassette: Plant hygromycin (with intron) resistance cassette

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter:35s + 5'UTR:Omega + CDS:hptIIwintron + 3'UTR/terminator:35s
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pICSL11056

    Plasmid

    #68258

    Purpose

    Level 1 Golden Gate Cassette: Cas9 expression cassette for monocotyledonous plants

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter/5UTR: ZmUbi + CDS:SpCas9 + 3UTR/terminator:35s
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEPYC0CM0416

    Plasmid

    #154573

    Purpose

    Phytobricks Level 0 part

    Depositor

    Nicola Patron

    Article

    Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

    Insert

    MinSyn_110
    

    Use

    Synthetic Biology

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEPYC0CM0411

    Plasmid

    #154568

    Purpose

    Phytobricks Level 0 part

    Depositor

    Nicola Patron

    Article

    Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

    Insert

    MinSyn_105
    

    Use

    Synthetic Biology

    Available Since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEPQD0CM0296

    Plasmid

    #162305

    Purpose

    Level 0 CDS part for MoClo assembly, AATG-TTCG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    CDS, superfolder GFP, no stop codon
    

    Use

    Synthetic Biology

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEPQD0KN0750

    Plasmid

    #185627

    Purpose

    Modified tobacco rattle virus RNA2 acceptor vector that could be used for expression of sgRNAs, contains restriction enzyme sites for golden gate assembly using AarI

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    lacZ
    

    Use

    CRISPR and Synthetic Biology

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pICSL11060

    Plasmid

    #68264

    Purpose

    Level 1 Golden Gate Cassette: Cas9 expression cassette for plants (exemplified in Brassica oleracea)

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter/5UTR: CsVMV+ CDS:SpCas9 + 3UTR/terminator:35s
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pICSL12009

    Plasmid

    #68257

    Purpose

    Level 0 Golden Gate Part: Promoter and 5UTR Ubiquitin (Zea mays)

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter and 5UTR Ubiquitin (Zea mays)
    

    Use

    Synthetic Biology

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEPQD2KN0908

    Plasmid

    #177088

    Purpose

    MoClo Level 2 plasmid containing multiple transcriptional units for transient expression of P19 (cytosol, driven by nosP), along with CrDXS2, PaGPPS1, and CrGES (plastid, driven by 4x opaatB)

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    P19, CrDXS2, PaGPPS1, and CrGES
    

    Use

    Synthetic Biology

    Available Since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEPQDKN0248

    Plasmid

    #162313

    Purpose

    Reporter plasmid for T7-driven E. coli cell-free protein synthesis with detection via HiBiT

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    T7prom-RBS-sfGFP-HiBiT
    

    Use

    Synthetic Biology

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge