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Showing: 1 - 20 of 1269 results

  1. pBetaActin-TUBB2a-mEos3.2-mEos3.2-mEos3.2

    Plasmid
    #191338
    Purpose
    Expresses triple fuorescently labeled beta tubulin to allow photoconversion of microtubule patches from green to red fluorescent with UV light in slice cultures
    Depositor
    Frank Bradke
    Article
    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:
    Insert
    TUBB2a-mEos3.2-mEos3.2-mEos3.2 (Tubb2a Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    191338-plasmid-map-sequence-id-370944

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  4. pmEos2-N1 GBP (GBP-mEos2)

    Plasmid
    #162876
    Purpose
    Expression in mammalian cells of GFP binding protein (GBP) tagged with photoconvertable protein mEos2 to track GFP proteins of interest to perform Fluorescent intrabody Localization Microscopy
    Depositor
    Frederic Meunier
    Article
    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p
    Insert
    GFP Binding Protein tagged with mEos2
    Use
    Tags
    mEos2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    162876-plasmid-map-sequence-id-321989

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  10. pmEos2-N1 PH-PLCd (wild-type) (PH-PLCd-mEos2)

    Plasmid
    #162877
    Purpose
    Expression in mammalian cells of Plekstrin-homology domain of Phospholipase C delta tagged with mEos2 to perform sptPALM
    Depositor
    Frederic Meunier
    Article
    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p
    Insert
    pleckstrin homology domain of PLCδ1 (phospholipase C-δ1) (PLCD1 Human)
    Use
    Tags
    mEos2
    Expression
    Mammalian
    Mutation
    Insert consists of AA1-170
    Promoter
    Available since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    162877-plasmid-map-sequence-id-321985

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  15. MmEos3.2

    Plasmid
    #203754
    Purpose
    Encodes the membrane anchor of Src including the first 15 resideues of the N terminus, with 1 myristoylation and short polybasic sequence, with mEos3.2 fused to the C terminus. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    Src15 n-term (SRC Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    203754-plasmid-map-sequence-id-403344

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  19. mEos3.2GG

    Plasmid
    #203756
    Purpose
    Encodes a modified version of the membrane anchor of KRas, including the last 16 residues of the C terminus, with mEos3.2 fused to the N terminus. The base pairs encoding the CAAX box for KRas were replaced by the CAAX box from rap1B, resulting in a geranylgeranylation instead of farnesylation. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    KRas c-term (KRAS Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    203756-plasmid-map-sequence-id-404785

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  20. mEos3.2GPI

    Plasmid
    #203759
    Purpose
    Encodes the fluorescent protein mEos3.2 followed by the C-terminal sequence derived from CD58 encoding a GPI-attachment signal. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    CD58 c-term (CD58 Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    203759-plasmid-map-sequence-id-404788

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Showing: 1 - 20 of 1269 results